A5086784077- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Acute Myeloid Leukemia Research
- Blood transfusion and management
- Mesenchymal stem cell research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Blood groups and transfusion
- Transplantation: Methods and Outcomes
- Chronic Lymphocytic Leukemia Research
- Viral Infectious Diseases and Gene Expression in Insects
- Blood donation and transfusion practices
- Neutropenia and Cancer Infections
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Bacterial Identification and Susceptibility Testing
- Blood properties and coagulation
- Acute Lymphoblastic Leukemia research
- Organ and Tissue Transplantation Research
- Infective Endocarditis Diagnosis and Management
- Polyomavirus and related diseases
- Pluripotent Stem Cells Research
- Trauma and Emergency Care Studies
UPMC Hillman Cancer Center
2022-2024
University of Pittsburgh
2023-2024
University of Pittsburgh Medical Center
2022-2023
Cornell University
2015-2022
Weill Cornell Medicine
2017-2022
New York Hospital Queens
2015-2020
NewYork–Presbyterian Hospital
2015-2020
Presbyterian Hospital
2015-2020
Washington University in St. Louis
2012-2015
The University of Texas MD Anderson Cancer Center
2007
Adoptive transfer of high-affinity chimeric antigen receptor (CAR) T cells targeting hematological cancers has yielded impressive clinical results. However, safety concerns regarding target expression on healthy tissue and poor efficacy have hampered application to solid tumors. Here, a panel affinity-variant CARs were constructed overexpressed ICAM-1, broad tumor biomarker, using its physiological ligand, LFA-1. Anti-tumor cell potency in vitro was directly proportional CAR affinity ICAM-1...
Abstract Immune checkpoint inhibition has shown success in treating metastatic cutaneous melanoma but limited efficacy against uveal melanoma, a rare variant arising from the immune privileged eye. To better understand this resistance, we comprehensively profile 100 human metastases using clinicogenomics, transcriptomics, and tumor infiltrating lymphocyte potency assessment. We find that over half of these harbor lymphocytes with potent autologous specificity, despite low mutational burden...
While the majority of thyroid cancer patients are easily treatable, those with anaplastic or poorly differentiated recurrent carcinomas have a very poor prognosis median survival less than year. Previously, we shown significant correlation between ICAM-1 overexpression and malignancy in cancer, pioneered use targeted CAR T cells as novel treatment modality. For clinical translation this modality, designed possessing micromolar rather nanomolar affinity to avoid cytotoxicity normal basal...
Abstract We administered severe acute respiratory syndrome coronavirus-2 viral-specific T cells (VSTs) under emergency investigational new drug applications to 6 immunocompromised patients with persistent coronavirus disease 2019 (COVID-19) and characterized clinical virologic responses. Three had partial responses after failing other therapies but then died. Two completely recovered, the role of VSTs in recovery was unclear due concomitant use antivirals. One patient not responded 2 courses...
Abstract: Massive transfusion protocols (MTPs) are established to provide rapid blood replacement in a setting of severe hemorrhage. Early optimal is essential sustain organ perfusion and oxygenation. There many variables consider when establishing an MTP, studies have prospectively evaluated different scenarios patient populations establish the best practices attain improved outcomes. The establishment utilization MTP challenging given ever-changing status during resuscitation efforts. Much...
BACKGROUND Current regulations do not require blood collection facilities to ask donors about cigarette smoking, and the prevalence of nicotine its metabolites in products is well established. Although smokers have higher hemoglobin (Hb) levels, smoking may adversely affect quality donated red cells through carboxyhemoglobin (COHb) content premature hemolysis. STUDY DESIGN AND METHODS Red cell (RBC) unit segments from 100 unique were tested for metabolite cotinine by mass spectrometry COHb...
Haplo-cord stem cell transplantation combines the infusion of CD34 selected hematopoietic progenitors from a haplo-identical donor with an umbilical cord blood (UCB) graft unrelated and allows faster count recovery, low rates disease recurrence chronic graft-versus-host (GVHD). But contribution to long-term transplant outcome remains unclear. We analyzed 39 recipients haplo-cord transplants acute myeloid leukemia (AML) myelodysplastic syndrome (MDS), engrafted in remission at 2 months....
Reduced-intensity conditioning (RIC) regimens, improved HLA matching, and better supportive care allow allogeneic stem cell transplant (alloSCT) to be offered older patients. Only a small percentage of eligible patients between ages 65 74 years actually undergo alloSCT, comprehensive outcome data from the aging population are still lacking. We examined who underwent alloSCT using melphalan-based RIC for hematologic malignancies at our institution. identified 125 than (median, 69; range, 66...
Rationale Methicillin‐resistant Staphylococcus aureus (MRSA) is one of major clinical pathogens responsible for both hospital‐ and community‐acquired infections worldwide. A delay in targeted antibiotic treatment contributes to longer hospitalization stay, higher costs, increasing in‐hospital mortality. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) has been integrated into the routine workflow microbial identification over past decade, it also...
Immune effector cells (IECs) include a broad range of immune capable modulating several disease states, including malignant and nonmalignant conditions. The growth in the use IECs as both investigational commercially available products requires medical institutions to develop workflows/processes safely implement deliver transformative therapy. Adding complexity this therapy are variety targets, diseases, sources, unique toxicities that patient experiences following IEC For over 25 years,...
BACKGROUND Daratumumab (DARA) is a human IgG1κ monoclonal antibody directed against CD38, approved for the treatment of multiple myeloma. As CD38 expressed on RBCs, DARA can interfere with pretransfusion testing. interference be negated by denaturation RBCs dithiothreitol (DTT) reagents. Because this in testing, our hospital implemented notification and testing/transfusion algorithm (NATTA) testing RBC product provision patients. This standardized approach combines DTT‐based selective...
Abstract Background Thawed Plasma (TP), plasma thawed and refrigerated for up to 5 days, is a commonly transfused product. This pilot study was conducted determine whether Solvent/Detergent‐treated stored 5‐days post‐thaw (T‐S/D) as efficacious TP. Study Design Methods single institution retrospective cohort analysis evaluated the efficacy of T‐S/D in reversing coagulopathies comparison Utilizing institution's electronic medical records, transfusion data were collected adult patients who...
PGF implies persistent cytopenia in the presence of predominant donor chimerism. We examined contributors to 104 HCT recipients who survived ≥100 days without relapse or major complications. Surrogate parameters for were: Hg <10 g/dl, RBC transfusion dependence, platelet count <20 × 109/L ANC < 0.5 109/L. All patients received T cell depletion with alemtuzumab ATG. The 2-year OS and PFS probabilities were 66%, 95%CI (56 − 75%) 51%, (41–60%) respectively. Fifty-four (52%) met one more...
6093 Background: ICAM-1 is a cell surface glycoprotein that typically expressed on endothelial cells and immune ligand for LFA-1 integrin. It also overexpressed in several malignancies, particular anaplastic advanced thyroid cancer. We designed an affinity tuned ICAM-1-directed CAR T (micromolar affinity) preferentially binds tumor spares normal cells. The express somatostatin receptor 2 (SSTR2), which allows tracking of vivo via DOTATATE PET/CT scan. Murine studies showed excellent...