A5062917293- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Immune Cell Function and Interaction
- Blood groups and transfusion
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Hematopoietic Stem Cell Transplantation
- Organ Transplantation Techniques and Outcomes
- Blood disorders and treatments
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- Blood transfusion and management
- Systemic Lupus Erythematosus Research
- Fetal and Pediatric Neurological Disorders
- Erythrocyte Function and Pathophysiology
- Infectious Aortic and Vascular Conditions
- Organ and Tissue Transplantation Research
- Vascular Procedures and Complications
- Spondyloarthritis Studies and Treatments
- Immunodeficiency and Autoimmune Disorders
- Psoriasis: Treatment and Pathogenesis
- Prenatal Screening and Diagnostics
- Blood donation and transfusion practices
- Single-cell and spatial transcriptomics
Aix-Marseille Université
2016-2025
Établissement Français du Sang
2018-2025
Centre National de la Recherche Scientifique
2016-2024
Hôpital Nord
2024
Délégation Régionale Provence-Alpes-Côte d’Azur et Corse
2022-2023
Hôpital de la Timone
2023
Centre de Recherche en Neurobiologie - Neurophysiologie de Marseille
2016
Objectives: The aim of this study was to assess the HLA alloreactivity cold-stored saphenous vein allografts (CSVAs) by identifying production donor-specific antibodies (DSAs) in recipient. secondary objective evaluate CSVA rejection-related complications, such as thrombosis and/or aneurysmal degeneration Methods: This a single-center, prospective, experimental before-and-after which included participants undergoing placement, either create vascular access (VA) for hemodialysis or lower limb...
Fc gamma receptors (FcγRs) play a major role in the regulation of humoral immune responses. Single-nucleotide polymorphisms (SNPs) FCGR2A and FCGR3A can impact expression level, IgG affinity function CD32 CD16 FcγRs response to their engagement by fragment IgG. The isoform encoded [158V/V] controls intensity antibody-dependent cytotoxic alloimmune responses natural killer cells (NK) has been identified as susceptibility marker predisposing patients cardiac allograft vasculopathy after heart...
Hematopoietic stem cell transplantation (HSCT) is a curative treatment for most hematologic diseases. To evaluate the level of donor engraftment, chimerism must be carefully monitored after HSCT. Short tandem repeats, quantitative PCR (qPCR), and, more recently, digital (dPCR) are widely used to determine proportions and recipient cells The screening quantification have been evaluated by 2 new methods: ready-to-use next-generation sequencing (NGS)-based method using Devyser ChimerismNGS kit...
Quantification of chimerism showing the proportion donor in a recipient is essential for follow-up hematopoietic stem cell transplantation but can also be useful to document an immune tolerance situation after solid organ transplantation. Historically, has been quantified from genomic DNA, with technological advances, donor-derived cell-free DNA seems particularly relevant transplantation.The reference method was until recently short tandem repeat technique, new innovative techniques as...
Background Many studies have reported the relevance of donor-derived cfDNA (dd-cfDNA) after lung transplantation (LTx) to diagnose and monitor acute rejection (AR) or chronic infection (INF). However, analysis fragment size has not been studied. The aim this study was determine clinical dd-cfDNA profiles in events (AR INF) during first month LTx. Methods This prospective, single-center includes 62 LTx recipients at Marseille Nord Hospital, France. Total quantification performed by...
ABSTRACT HLA‐A*23:147 differs from HLA‐A*23:13 allele by one nucleotide substitution in codon 283 exon 5.
Abstract Background Long-term outcomes of lung transplantation (LTx) remain hampered by chronic allograft dysfunction (CLAD). Matrix metalloproteinase 9 (MMP-9) is a secretory endopeptidase identified as key mediator in fibrosis processes associated with CLAD. The objective this study was to investigate whether plasma MMP9 levels may be prognostic CLAD development. Methods Participants were selected from the Cohort Lung Transplantation (COLT) for which biocollection associated. We considered...
Non-invasive molecular analysis of cell-free DNA (cfDNA) became a sensitive biomarker for monitoring organ transplantation or detection fetal (cffDNA) in noninvasive prenatal test. In this study, we compared the efficiencies four (semi)-automated cfDNA isolation instruments using their respective kit: MagNA Pure 24 (Roche®), IDEAL (IDSolution®), LABTurbo (Taigen®) and Chemagic 360 (Perkin Elmer®). The was isolated from 5 plasma samples Rhesus D (RhD)-cffDNA maternal plasmas. were quantified...
Few data exist on the role of genetic factors involving HLA system response to Covid‐19 vaccines. Moving from suggestions a previous study investigating association some alleles with humoral BNT162b2, we here compared allele frequencies among weak ( n = 111) and strong 123) responders, defined as those healthcare workers lowest highest anti‐Spike antibody levels after vaccination. Individuals clinical history or positive anti‐nucleocapside antibodies were excluded. We found common...
Fifty percent of all patients with cancer worldwide require radiotherapy. In the case brain tumors, despite improvement in precision radiation delivery proton therapy, studies have shown structural and functional changes brains treated protons. The molecular pathways involved generating these effects are not completely understood. this context, we analyzed impact exposure central nervous system area Caenorhabditis elegans a focus on mitochondrial function, which is potentially implicated...
HLA-DQA1*03:01:12 differs from HLA-DQA1*03:01:01:01 by one nucleotide substitution in codon 21 exon 2.
HLA-B*07:482 differs from HLA-B*07:02:01:01 allele by one nucleotide substitution in codon 285 exon 5.
HLA-A*33:246 differs from HLA-A*33:01:01:01 allele by one nucleotide substitution in codon 135 exon 3.
Forty‐three novel HLA‐H alleles, including 32 identified in cohorts of French donors for Hematopoietic Stem Cell Transplantation, have been characterized by Next‐Generation Sequencing (NGS) using a long range PCR approach. A phylogenetic study confirms three main clades.
Allogeneic hematopoietic stem cell transplantation (allo-SCT) is today an established cure for many malignant and non-malignant hematological disorders. The post-allo-SCT monitoring very important in the engraftment phase to detect graft failure, but also following of post-SCT risk disease relapse disease. quantification chimerism determination proportion donor cells recipient blood after allo-SCT. Currently, two complementary molecular biology techniques, STR PCR qPCR methods, are routinely...
The INTERCEPTTM Blood System (Intercept System, Cerus Europe BV, Amersfoort, the Netherlands) has been used to reduce or inactivate pathogen load in platelet concentrates France for three years.After comparing transfusion efficiency between pathogen-reduced platelets (PR_PLT) and untreated products (U_PLT), our single-center observational study assessed effectiveness of PR_PLT prevention bleeding therapeutic treatment WHO grade 2 176 patients undergoing chemotherapy with curative intent...
Red blood cell (RBC) transfusion remains a critical component in caring for the acute and chronic complications of sickle disease (SCD). Patient alloimmunisation is main limitation transfusion, which can worsen anaemia lead to delayed haemolytic reaction or deadlock. Although biological risk factors have been identified immunisation, patient difficult predict. We aimed characterise genetic optimise management products compatible with extended antigen matching ensure self-sufficiency labile...