A5015513859- DNA Repair Mechanisms
- Herpesvirus Infections and Treatments
- Cell Adhesion Molecules Research
- DNA and Nucleic Acid Chemistry
- Proteoglycans and glycosaminoglycans research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- RNA and protein synthesis mechanisms
- Vector-Borne Animal Diseases
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Estrogen and related hormone effects
- Transgenic Plants and Applications
- Prostate Cancer Treatment and Research
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Polyomavirus and related diseases
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Hepatitis C virus research
- Toxoplasma gondii Research Studies
National Institutes of Health
2021-2024
National Institute of Environmental Health Sciences
2021-2024
University of North Carolina at Chapel Hill
2013-2023
Triangle
2021
UNC Lineberger Comprehensive Cancer Center
2013-2015
Yale University
2010-2013
Université Paris-Est Créteil
2007-2011
Centre National de la Recherche Scientifique
2007-2011
Laboratoire de Recherche sur la Croissance Cellulaire, la Réparation et la Régénération Tissulaires
2007-2011
Université Paris Cité
2010-2011
The fidelity of DNA polymerases depends on conformational changes that promote the rejection incorrect nucleotides before phosphoryl transfer. Here, we combine single-molecule FRET with use polymerase I and various mutants to highlight mechanisms by which active-site side chains influence transitions free-energy landscape underlie decisions in synthesis. Ternary complexes high derivatives complementary dNTPs adopt mainly a fully closed conformation, whereas conformation value between those...
DNA polymerases catalyze the incorporation of deoxynucleoside triphosphates into a growing chain using pair Mg2+ ions, coordinated at active site by two invariant aspartates, whose removal mutation typically reduces polymerase activity to barely detectable levels. Using stopped-flow fluorescence assays that we developed previously, have investigated role carboxylate ligands, Asp705 and Asp882, I (Klenow fragment) in early prechemistry steps prepare for catalysis. We find neither is required...
Abstract Background Heparin affin regulatory peptide (HARP), also called pleiotrophin, is a heparin-binding, secreted factor that overexpressed in several tumours and associated to tumour growth, angiogenesis metastasis. The C-terminus part of HARP composed amino acids 111 136 particularly involved its biological activities we previously established synthetic the same (P111-136) was capable inhibiting HARP. Here evaluate ability P111-136 inhibit vitro vivo growth human cell line PC-3 which...
The accuracy of high-fidelity DNA polymerases such as polymerase I (Klenow fragment) is governed by conformational changes early in the reaction pathway that serve fidelity checkpoints, identifying inappropriate template–nucleotide pairings. fingers-closing transition (detected a fluorescence resonance energy transfer-based assay) unique outcome binding correct incoming nucleotide, both complementary to templating base and with deoxyribose (rather than ribose) sugar structure. Complexes...
Hepatitis C virus (HCV) requires two cellular factors, microRNA-122 (miR-122) and poly(C) binding protein 2 (PCBP2), for optimal replication. These host factors compete to the 5' end of single-stranded RNA genome regulate viral replication cycle. To understand how they interact with RNA, we measured affinities both an probe representing 45 nucleotides HCV (HCV45). Isothermal titration calorimetry revealed two, unequal miR-122 sites in HCV45, high-affinity (S1) low-affinity (S2), differing...
Abstract Background Pleiotrophin, also known as HARP (Heparin Affin Regulatory Peptide) is a growth factor expressed in various tissues and cell lines. Pleiotrophin participates multiple biological actions including the induction of cellular proliferation, migration angiogenesis, involved carcinogenesis. Recently, we identified characterized several pleiotrophin proteolytic fragments with activities similar or opposite to that pleiotrophin. Here, investigated P(122-131), synthetic peptide...
During lytic infection, herpes simplex virus (HSV) DNA is replicated by a mechanism involving recombination. For instance, replication of the HSV-1 genome produces X- and Y-branched structures, reminiscent recombination intermediates. HSV-1‘s machinery includes trimeric helicase–primase composed helicase (UL5) primase (UL52) subunits third subunit, UL8. UL8 has been reported to stimulate activities complex in presence ICP8, an protein that functions as annealase, binds complementary...