A5015244514- Immune cells in cancer
- Immune Response and Inflammation
- Cancer Cells and Metastasis
- Pneumonia and Respiratory Infections
- Reproductive System and Pregnancy
- Inflammation biomarkers and pathways
- Bacterial Infections and Vaccines
- Cancer Immunotherapy and Biomarkers
- Advanced Breast Cancer Therapies
- Chemokine receptors and signaling
- Streptococcal Infections and Treatments
- Immunotherapy and Immune Responses
- S100 Proteins and Annexins
- Infant Nutrition and Health
- Cancer Genomics and Diagnostics
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Breast Cancer Treatment Studies
- Fatty Acid Research and Health
- Cancer, Stress, Anesthesia, and Immune Response
- Inflammasome and immune disorders
- Wnt/β-catenin signaling in development and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Biomarkers in Disease Prognosis
Lund University
2014-2025
Skåne University Hospital
2012-2015
Abstract Background Wnt proteins are important for developmental processes and certain diseases. WNT5A is a non-canonical protein that previously has been shown to play role in the progression of malignant melanoma. High expression melanoma tumors correlates formation distant metastasis poor prognosis. This partly described by findings cell lines increases migration invasion. Methods Malignant were treated with rWNT5A or siRNA, mRNA versus levels soluble mediators measured using RT-PCR,...
Abstract Triple-negative (TN) breast cancers (ER − PR HER2 ) are highly metastatic and associated with poor prognosis. Within this subtype, invasive, stroma-rich tumours infiltration of inflammatory cells even more aggressive. The effect myeloid on reactive stroma formation in TN cancer is largely unknown. Here, we show that primary human monocytes have a survival advantage, proliferate vivo develop into immunosuppressive expressing the myeloid-derived suppressor cell marker S100A9 only...
Myeloid-derived suppressor cells (MDSCs) are highly immunosuppressive myeloid cells, which increase in cancer patients. The molecular mechanism behind their generation and function is unclear. Whereas granulocytic-MDSCs correlate with poor overall survival breast cancer, the presence relevance of monocytic-MDSCs (Mo-MDSCs) unknown. Here we report for first time an enrichment functional blood Mo-MDSCs patients before they acquire a typical Mo-MDSC surface phenotype. A clear population cell...
ABSTRACT The causative microorganisms dictate the type of MDSC generated in sepsis patients, and a large proportion PMN-MDSCs gram-positive includes immunosuppressive myeloid blasts. MDSCs constitute heterogeneous population immature cells that potently suppress immune responses. They were identified originally cancer patients have since been reported to occur also chronic inflammation, autoimmunity, even bacterial infections. Human are commonly divided into Mo-MDSCs granulocytic (PMN-MDSCs)...
A well-orchestrated inflammatory reaction involves the induction of effector functions and, at a later stage, an active downregulation this potentially harmful process. In study we show that under proinflammatory conditions noncanonical Wnt protein, Wnt5a, induces immunosuppressive macrophages. The suppressive phenotype induced by Wnt5a is associated with IL-10 and inhibition classical TLR4-NF-κB signaling. Interestingly, closely resembles observed in reprogrammed monocytes sepsis patients....
Abstract The overall aim of this prospective study was to delineate the role monocytic myeloid-derived suppressor cells (Mo-MDSCs) in patients with metastatic breast cancer (MBC). MDSCs are a heterogeneous group immunosuppressive often enriched different malignancies which hold prognostic and predictive value for clinical outcomes. Here, we assessed significance Mo-MDSCs 54 de novo or distant recurrent MBC. We show that high levels significantly correlated MBC (metastatic disease at initial...
Toll-like receptors (TLRs) are a family of pattern recognition that expressed on cells the innate immune system. The ligands can be pathogen derived (pathogen associated molecular patterns; PAMPs) or endogenous (damage patters; DAMPs) when bound induces activation nuclear factor kappa B (NF-κB) and transcription pro-inflammatory genes. TLRs have also been discovered in various malignant cell types, but with unknown function. In this study we performed detailed analysis TLR co-receptor...
Abstract Background Metastatic breast cancer (MBC) is a challenging disease, and despite new therapies, prognosis still poor for majority of patients. There clinical need improved prognostication where immuno-oncology markers can provide important information. The aim this study was to evaluate serum in MBC patients their respective relevance prediction survival. Patients methods We investigated broad panel 92 proteins from 136 included prospective observational (NCT01322893) with long-term...
Background: Metastatic breast cancer (MBC) is the main cause of cancer-related death. The outcome MBC varies, and there a lack biomarkers to aid in prognostication. primary aim this study was evaluate prognostic value gene expression (GEX) signatures tumor (PT) distant metastasis (DM) for progression-free survival (PFS) overall (OS). secondary describe GEX changes through evolution identify subtypes. Methods: RNA extracted from PT, lymph node (LNM), DM patients prospective observational ( n...
Metastatic breast cancer (MBC) is generally considered an incurable disease and even though new treatments are available, the median survival approximately three years. The introduction of immune therapies for MBC highlights importance system in progression treatment. CD163
Background Certain species from the normal respiratory tract microbiota have recently been proposed to positively influence human health. Corynebacterium propinquum and C. pseudodiphtheriticum (Corynebacteria) are two Gram-positive that frequently colonize upper strongly associate with a reduced incidence of infections. The specific role Corynebacteria during health disease is, however, largely uncharacterized. Method Respiratory epithelial cells NCI-H292 BALB/cByJ mice were inoculated (...
Abstract Innate immune responses are rapid, dynamic and highly regulated to avoid overt reactions. This regulation is executed by innate tolerance mechanisms that remain obscure. Wnt5a a signalling protein mainly involved in developmental processes cancer. The effect of on inflammatory myeloid cells controversial. Here, we combine primary cell cultures, vitro binding studies, mass spectrometry Drosophila modelling show direct ligand toll-like receptor (TLR) 2 4. promotes MyD88-non-canonical...
Tumors affect the immune system, locally and systemically. The frequencies of specific circulating cell populations correlate with disease progression as well prognosis patients. Although largely neglected, conventional antitumoral therapies often possess immunomodulatory properties levels populations. Most information, however, derive from animal or in vitro studies. As this could impact response to therapy, further studies effects treatment on cells patients are warranted. In pilot study,...
Local tumor-associated immune cells hold prognostic and predictive value in various forms of malignancy. The role systemic, circulating leukocytes is, however, not well-characterized. In this prospective explorative study, we aim to delineate the clinical relevance a broad panel 32 patients with newly diagnosed metastatic breast cancer (MBC) before start systemic treatment. Freshly isolated peripheral blood mononuclear (PBMCs) were analyzed by flow cytometry evaluated for potential...
Wnt5a is a non-canonical Wnt protein that expressed at elevated levels in inflammatory conditions. Its role inflammation remains unclear, although it known higher level monocyte-derived myeloid dendritic cells (Mo-mDCs) than monocytes and macrophages. The function of (DCs) relatively unexplored. Here, we found under Mo-mDC culture conditions, inhibited the generation CD14(⁺/low) Mo-mDCs while promoting CD14⁺/⁺⁺ CD16⁺ monocytes. We could further show stimulation with rWnt5a induced rapid IL-6...
Myeloid-derived suppressor cells (MDSCs) are known to contribute immune evasion in cancer. However, the function of human granulocytic (G)-MDSC subset during tumor progression is largely unknown, and there no established markers for their identification specimens. Using gene expression profiling, mass cytometry, microarrays, we here demonstrate that G-MDSCs occur as neutrophils at distinct maturation stages, with a disease-specific profile. derived from patients metastatic breast cancer...
Otitis media with effusion (OME) is a common inflammatory disease that primarily affects children. OME defined as chronic low-grade inflammation of the middle ear (ME), without any signs infection and persisting in ME for more than 3 months. The precise pathogenesis is, however, not fully understood. Here, we comprehensively characterized compared host immune responses (inflammatory cells mediators) overall microbial community composition (microbiota) present matched (MEE) samples, external...
Immune activation is a regular feature of sepsis, but the incidence and nature ensuing inflammation-resolving immunosuppressive component less well understood. In this study, we compared immunoregulatory markers on blood leukocytes from patients with Gram-negative or Gram-positive sepsis septic shock, to severe virosis healthy controls. To end, 32 including ten cases 12 were analysed by flow cytometry for expression levels monocyte HLA-DR, CD11c, CD14 CD40, frequencies CD163+-suppressive...
Patients with estrogen receptor α positive (ERα+) breast cancer can respond to endocrine therapy, but treatment resistance is common and associated downregulation of ERα expression in the dormant residual cells. Here we show, using long-term NSG xenograft models human primary monocytes, vitro cell cultures tumors from patients, that macrophage derived tumor necrosis factor alpha (TNFα) downregulates cells via inactivation transcription Forkhead box O 3a (FOXO3a). Moreover, presence...