A5058723944- Advanced Glycation End Products research
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Biochemical effects in animals
- Alcohol Consumption and Health Effects
- Diet, Metabolism, and Disease
- Natural Antidiabetic Agents Studies
- Renin-Angiotensin System Studies
- Fatty Acid Research and Health
- Neuroscience of respiration and sleep
- Pneumocystis jirovecii pneumonia detection and treatment
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Sulfur Compounds in Biology
- Prostate Cancer Treatment and Research
- Amino Acid Enzymes and Metabolism
- Diet and metabolism studies
- Plant and fungal interactions
- Biochemical Acid Research Studies
- Pancreatic function and diabetes
- Muscle metabolism and nutrition
- Systemic Sclerosis and Related Diseases
- Inflammatory Myopathies and Dermatomyositis
- Antioxidant Activity and Oxidative Stress
- Regulation of Appetite and Obesity
University of Saskatchewan
2011-2024
Biotech Park
2023
Emory University
2021-2022
Wake Forest University
2021
Forest Institute
2021
Institute of Cancer Research
2019-2021
Emory University Hospital
2021
University of Pennsylvania
2018-2020
Philadelphia VA Medical Center
2019
St.John's Medical College Hospital
2017
Nitric oxide produced in endothelial cells affects vascular tone. To investigate the role of nitric synthase (eNOS) blood pressure regulation, we have generated mice heterozygous (+/−) or homozygous (−/−) for disruption eNOS gene. Immunohistochemical staining with anti-eNOS antibodies showed reduced amounts protein +/− and absence −/− mutant mice. Male female all three genotypes were indistinguishable general appearance histology, except that had lower body weights than +/+ Blood pressures...
Patients with type 2 diabetes mellitus (T2DM) have reduced platelet inhibition compared non-diabetics following P2Y12 receptor blockade. Whether of signalling can be enhanced by adjunctive treatment cilostazol in T2DM patients is unknown. The aim this pilot study was to assess the functional impact on standard aspirin and clopidogrel treatment. This a prospective, double-blind, double-dummy, placebo-controlled, randomized, cross-over function study. dual antiplatelet therapy were assigned...
OBJECTIVE The incidence of high dietary carbohydrate-induced type 2 diabetes is increasing worldwide. Methylglyoxal (MG) a reactive glucose metabolite and major precursor advanced glycation end products (AGEs). MG levels are elevated in diabetic patients. We investigated the effects chronic administration on tolerance β-cell insulin secreting mechanism 12-week-old male Sprague-Dawley rats. RESEARCH DESIGN AND METHODS (60 mg/kg/day) or 0.9% saline was administered by continuous infusion with...
The pathogenic process of diabetes mellitus is associated with increased methylglyoxal (MG). MG causes non-enzymic glycation proteins to form irreversible advanced endproducts (AGEs). However, the correlation between and essential hypertension unknown. aim present study was investigate whether MG, MG-induced AGEs, oxidative stress were in aorta spontaneously hypertensive rats (SHR) an formation related AGEs correlated development high blood pressure these rats.High-performance liquid...
Endothelial dysfunction is a feature of hypertension and diabetes. Methylglyoxal (MG) reactive dicarbonyl metabolite glucose its levels are elevated in spontaneously hypertensive rats diabetic patients. We investigated if MG induces endothelial whether scavengers can prevent induced by high concentrations.Endothelium-dependent relaxation was studied aortic rings from Sprague-Dawley rats. also used cultured rat human umbilical vein cells. The measured HPLC Western blotting assay kits were...
Alagebrium is a breaker of cross-links in advanced glycation endproducts. However, the acute effects alagebrium on methylglyoxal (MG), major precursor endproducts have not been reported. MG highly reactive endogenous metabolite, and its levels are elevated diabetic patients. We investigated whether attenuated exogenous plasma levels, glucose tolerance distribution administered different organs Sprague-Dawley rats.We measured (by HPLC), tolerance, adipose tissue uptake, GLUT4, insulin...
Here we show that the relaxation induced by stimulation of vagus nerve in presence cholinergic (muscarinic) and adrenergic blockade isolated stomach guinea pig is mediated nitric oxide (NO). This substantiated inhibition vagal NG-monomethyl-L-arginine, an inhibitor NO synthesis. The effect NG-monomethyl-L-arginine was partially reversed coincubation with L-arginine but not D-arginine. activates soluble guanylate cyclase, prevented methylene blue, further supporting our conclusions. relaxant...
Methylglyoxal is a reactive dicarbonyl intermediate of metabolism produced in the body. It reacts with certain proteins and forms damaging advanced glycation endproducts (AGEs) such as N epsilon-carboxyethyl-lysine (CEL) epsilon-carboxymethyl-lysine (CML). Increased methylglyoxal levels are found diabetes mellitus associated hypertension development spontaneously hypertensive rats (SHR). The purpose this study was to investigate whether increased endogenous formation methylglyoxal-induced...
Methylglyoxal (MG) overproduction has been reported in metabolic syndrome with hyperglycaemia (diabetes) or without (hypertension), and the underlying mechanism was investigated. Contributions of different pathways enzymes to MG formation were evaluated aorta cultured vascular smooth muscle cells (VSMCs). In all four animal models syndrome, i.e. chronically fructose-fed hypertensive Sprague–Dawley rats, spontaneously obese non-diabetic Zucker diabetic serum aortic fructose levels increased,...
The current epidemic of obesity and type 2 diabetes is attributed to a high carbohydrate diet, containing mainly fructose corn syrup sucrose. More than two thirds diabetic patients have hypertension. Methylglyoxal highly reactive dicarbonyl generated during glucose metabolism, major precursor advanced glycation end products (AGEs). Plasma methylglyoxal levels are increased in hypertensive rats patients. Our aim was examine the methylglyoxal, mediators renin angiotensin system blood pressure...
Methylglyoxal (MG), a metabolite of glucose, and MG-induced advanced glycation endproducts (AGEs) are causatively associated with vascular complications diabetes mellitus. We have previously reported elevated levels MG AGEs in spontaneously hypertensive rats (SHR). The purpose this study was to investigate the causative role pathogenesis hypertension SHR. Young SHR were treated an AGE inhibitor, aminoguanidine, for 9 weeks. HPLC used determine plasma aortic reduced glutathione levels. AGEs,...
Methylglyoxal (MG), a reactive metabolite of glucose, has high affinity for arginine and is precursor advanced glycation endproducts (AGEs). We tested the hypothesis that l-arginine, its inactive isomer d-arginine, can efficiently scavenge MG, administered exogenously or produced endogenously from attenuate harmful effects including endothelial dysfunction oxidative stress by an nitric-oxide synthase (eNOS)-independent mechanism. used isolated aortic rings 12-week-old male Sprague-Dawley...
To investigate the mechanism underlying observation that infusion of growth hormone secretagogue peptide, ghrelin, produces a decrease in mean arterial pressure (MAP) with no change heart rate.The effect single bolus ghrelin (12 nmol/kg intravenously) on changes MAP and rate was determined 12-week-old male anaesthetized Sprague-Dawley rats subjected to pretreatment either nitric oxide synthase (NOS) inhibitor, N-nitro-L-arginine methyl ester (L-NAME; 0.7 mg/ml by mouth for 5 days), or...