A5062689011- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Anaerobic Digestion and Biogas Production
- Bioenergy crop production and management
- Eicosanoids and Hypertension Pharmacology
- Fatty Acid Research and Health
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Carcinogens and Genotoxicity Assessment
- Metabolomics and Mass Spectrometry Studies
- Potato Plant Research
- Glutathione Transferases and Polymorphisms
- Mass Spectrometry Techniques and Applications
- ATP Synthase and ATPases Research
- Advanced Chemical Sensor Technologies
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Community Ecology and Physiology
- Metabolism and Genetic Disorders
- Pharmaceutical and Antibiotic Environmental Impacts
- Bioinformatics and Genomic Networks
- Adipose Tissue and Metabolism
- Enzyme Production and Characterization
- biodegradable polymer synthesis and properties
- Cancer, Hypoxia, and Metabolism
University of Wisconsin–Madison
2002-2020
Great Lakes Bioenergy Research Center
2010-2020
Energy Center of Wisconsin
2012-2020
Michigan State University
2015
Biotechnology Research Center
2010
Roswell Park Comprehensive Cancer Center
2002-2003
ABSTRACT Fermentation of cellulosic and hemicellulosic sugars from biomass could resolve food-versus-fuel conflicts inherent in the bioconversion grains. However, inability to coferment glucose xylose is a major challenge economical use lignocellulose as feedstock. Simultaneous cofermentation glucose, xylose, cellobiose problematic for most microbes because represses utilization other saccharides. Surprisingly, ascomycetous, beetle-associated yeast Spathaspora passalidarum , which ferments...
The inability of native Saccharomyces cerevisiae to convert xylose from plant biomass into biofuels remains a major challenge for the production renewable bioenergy. Despite extensive knowledge regulatory networks controlling carbon metabolism in yeast, little is known about how reprogram S. ferment at rates comparable glucose. Here we combined genome sequencing, proteomic profiling, and metabolomic analyses identify characterize responsible mutations series evolved strains capable...
Significance The search for new antifungal compounds is struggling to keep pace with emerging fungicide resistance. Through chemoprospecting of an untapped reservoir inhibitory compounds, lignocellulosic hydrolysates, we have identified a previously undescribed agent, poacic acid. Using both chemical genomics and morphological analysis together the first time, our knowledge, cellular target acid as β-1,3-glucan. its action on glucan layer fungal cell walls, natural agent against economically...
The inability of the yeast Saccharomyces cerevisiae to ferment xylose effectively under anaerobic conditions is a major barrier economical production lignocellulosic biofuels. Although genetic approaches have enabled engineering S. convert efficiently into ethanol in defined lab medium, few strains are able from hydrolysates absence oxygen. This limited conversion believed result small molecules generated during biomass pretreatment and hydrolysis, which induce cellular stress impair...
Abstract Ethanol production from lignocellulosic biomass holds promise as an alternative fuel. However, industrial stresses, including ethanol stress, limit microbial fermentation and thus prevent cost competitiveness with fossil fuels. To identify novel engineering targets for increased tolerance, we took advantage of natural diversity in wild Saccharomyces cerevisiae strains. We previously showed that S288c-derived lab strain cannot acquire higher tolerance after a mild pretreatment, which...
The physiology of ethanologenic Escherichia coli grown anaerobically in alkali-pretreated plant hydrolysates is complex and not well studied. To gain insight into how E. responds to such hydrolysates, we studied an K-12 ethanologen fermenting a hydrolysate prepared from corn stover pretreated by ammonia fiber expansion. Despite the high sugar content (∼6% glucose, 3% xylose) relatively low toxicity this hydrolysate, ceased growth long before glucose was depleted. Nevertheless, cells remained...
Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are suite aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed microbial-mediated strategy removing these compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris...
Highlights•Ptc7p is an active, conserved PP2C-type mitochondrial matrix phosphatase•Loss of Ptc7p causes respiratory defects and enhanced select phosphorylation•Cit1p a target whose activity diminished by phosphorylation•Dephosphorylation reactivates Cit1p likely enabling its requisite dimerizationSummaryProper maintenance essential for metabolic homeostasis. Widespread phosphorylation proteins may be important element this process; yet, little known about which enzymes control or...
This study investigated the capacity of conjugated linoleic acids (CLA) to reduce ex vivo antigen-induced release eicosanoids in a type I hypersensitivity model. Guinea pigs were fed diet containing 0.25% safflower oil (control) or CLA [43% trans( t)10, cis ( c)12; 41% c9, t11/ t9, c11 18:2] for 2 wk before and during sensitization ovalbumin (OVA). Lungs, tracheas, bladders incubated physiological saline solution (PSS) 1 h (basal mediator release) challenged with OVA (0.01 g/l PSS) (mediator...
Microbial conversion of lignocellulosic feedstocks into biofuels remains an attractive means to produce sustainable energy. It is essential hydrolysates in a consistent manner order study microbial performance different feedstock hydrolysates. Because the potential introduce contamination from untreated biomass or at various points during process, it can be difficult control sterility hydrolysate production. In this study, we compared produced AFEX-pretreated corn stover and switchgrass...
Efficient microbial conversion of lignocellulosic hydrolysates to biofuels is a key barrier the economically viable deployment biofuels. A chief contributor this impact on processes and energy metabolism lignocellulose-derived inhibitors, including phenolic carboxylates, amides (for ammonia-pretreated biomass), aldehydes, furfurals. To understand bacterial pathways induced by inhibitors present in biomass hydrolysates, which are less well studied than acid-pretreated we developed exploited...
Interannual variability in precipitation, particularly drought, can affect lignocellulosic crop biomass yields and composition, is expected to increase biofuel yield variability. However, the effect of precipitation on downstream fermentation processes has never been directly characterized. In order investigate impact interannual climate production, corn stover switchgrass were collected during 3 years with significantly different profiles, representing a major drought year (2012) 2 average...
Abstract Increasing the diversity of lignocellulosic feedstocks accepted by a regional biorefinery has potential to improve environmental footprint facility; harvest, storage, and transportation logistics; economics. However, can vary widely in terms their biomass yields quality characteristics (chemical composition, moisture content, etc.). To investigate how biofuel cropping systems feedstock supply might affect process field‐scale ethanol yields, we processed experimentally quantified...
Fatty acids play many important roles in cells and also industrial processes. Furan fatty (FuFAs) are present the lipids of some plant, fish, microbial species appear to function as second messengers pathways that protect from membrane-damaging agents. We report here results chemical, genetic, synthetic biology experiments decipher biosynthesis monomethylated FuFA, methyl 9-(3-methyl-5-pentylfuran-2-yl) nonanoate (9M5-FuFA), its dimethyl counterpart, 9-(3,4-dimethyl-5-pentylfuran-2-yl)...
Gamma valerolactone (GVL) treatment of lignocellulosic bomass is a promising technology for degradation biomass biofuel production; however, GVL toxic to fermentative microbes. Using combination chemical genomics with the yeast (Saccharomyces cerevisiae) deletion collection identify sensitive and resistant mutants, proteomics monitor protein abundance in presence GVL, we sought understand mechanism toxicity resistance goal engineering GVL-tolerant, xylose-fermenting yeast.Chemical genomic...
Box, H. C., Patrzyc, B., Dawidzik, J. Iijima, H., Freund, G., Higbee, A. and Budzinski, E. Detection Characterization of Formamido Lesions in DNA by Liquid Chromatography-Mass Spectrometry. Radiat. Res. 158, 538–542 (2002).DNA X-irradiated oxygenated aqueous solution produces the formamido lesion from breakdown pyrimidine nucleosides. This product inhibits hydrolysis nuclease P1 phosphoester bond 3′ to damaged nucleoside. Consequently, can be obtained an enzymatic digest as a modified...
Abstract Background Interannual variability in precipitation, particularly drought, can affect lignocellulosic crop biomass yields and composition, is expected to increase biofuel yield variability. However, the effect of precipitation on downstream fermentation processes has never been directly characterized. In order investigate impact interannual climate production, corn stover switchgrass were collected during three years with significantly different profiles, representing a major...