A5029000869- Biochemical Analysis and Sensing Techniques
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Olfactory and Sensory Function Studies
- Gut microbiota and health
- Advanced Chemical Sensor Technologies
- Bat Biology and Ecology Studies
- RNA and protein synthesis mechanisms
- Receptor Mechanisms and Signaling
- Cancer, Hypoxia, and Metabolism
- Sirtuins and Resveratrol in Medicine
- Neuroendocrine regulation and behavior
- Ovarian function and disorders
- Tea Polyphenols and Effects
- Pancreatic function and diabetes
- Diet and metabolism studies
- Food Chemistry and Fat Analysis
- Metabolism, Diabetes, and Cancer
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Fatty Acid Research and Health
- Vitamin D Research Studies
- Peroxisome Proliferator-Activated Receptors
- Biotin and Related Studies
- Diet, Metabolism, and Disease
University of Wisconsin–Madison
2013-2023
Resonance Research (United States)
2007-2018
Fitchburg State University
2017
Texas Tech University
2014
Isomark (United States)
2013
Icahn School of Medicine at Mount Sinai
2008
G protein-coupled receptors mediate responses to a myriad of ligands, some which regulate adipocyte differentiation and metabolism. The sweet taste T1R2 T1R3 are that function as carbohydrate sensors in buds, gut, pancreas. Here we report expressed throughout adipogenesis adipose tissues. Treatment mouse human precursor cells with artificial sweeteners, saccharin acesulfame potassium, enhanced adipogenesis. Saccharin treatment 3T3-L1 primary mesenchymal stem rapidly stimulated...
Hibernation is a mammalian strategy that uses metabolic plasticity to reduce energy demands and enable long-term fasting. Fasting mitigates winter food scarcity but eliminates dietary nitrogen, jeopardizing body protein balance. Here, we reveal gut microbiome–mediated urea nitrogen recycling in hibernating thirteen-lined ground squirrels ( Ictidomys tridecemlineatus ). Ureolytic microbes incorporate into metabolites are absorbed by the host, with reincorporated squirrel’s pool. Urea greatest...
Polycystic ovary syndrome (PCOS), a common female endocrinopathy, is complex metabolic of enhanced weight gain. The goal this pilot study was to evaluate differences between normal (n=10) and PCOS women via breath carbon isotope ratio, urinary nitrogen nuclear magnetic resonance (NMR)-determined serum metabolites. Breath stable isotopes measured by cavity ring down spectroscopy (CRDS) indicated diminished (p<0.030) lipid use as substrate during overnight fasting in compared women....
Hibernation is a wintertime survival strategy employed by certain mammals that, through torpor use, reduces overall energy expenditure and permits prolonged fasting. Although fasting resolves the challenge of winter food scarcity, it also removes dietary carbon, critical biomolecular building block. Here, we demonstrate process urea carbon salvage (UCS) in 13 lined ground squirrel, whereby reclaimed gut microbial ureolysis used reductive acetogenesis, producing acetate, short-chain fatty...
We present a robust method for monitoring the binding of ligands to heterodimeric (T1R2+T1R3) human sweet receptor (a family 3 GPCR receptor). The approach utilizes saturation transfer difference (STD) NMR spectroscopy with proteins expressed on surface epithelial kidney cells. preparation investigated by can contain either live cells or membranes isolated from these containing receptor. have used this confirm noncompetitive alitame and cyclamate determine that greatly reduced affinity...
Vitamin D receptor (VDR) is a member of the nuclear hormone superfamily. When bound to variety vitamin analogues, VDR manifests wide diversity physiological actions. The molecular mechanism by which different analogues cause specific responses not understood. published crystallographic structures ligand binding domain (VDR-LBD) complexed with ligands that have differential biological activities exhibited identical protein conformations. Here we report rat VDR-LBD (rVDR-LBD) in solution...
3-Iodothyronamine (T1 AM), an analog of thyroid hormone, is a recently discovered fast-acting endogenous metabolite. Single high-dose treatments T1 AM have produced rapid short-term effects, including reduction body temperature, bradycardia, and hyperglycemia in mice.The effect daily low doses (10 mg/kg) for 8 days on weight loss metabolism spontaneously overweight mice was monitored. The experiments were repeated twice (n = 4). Nuclear magnetic resonance (NMR) spectroscopy plasma real-time...
Brazzein is a small, heat‐stable, intensely sweet protein consisting of 54 amino acid residues. Based on the wild‐type brazzein, 25 brazzein mutants have been produced to identify critical regions important for sweetness. To assess their sweetness, psychophysical experiments were carried out with 14 human subjects. First, results suggest that residues 29–33 and 39–43, plus residue 36 between these stretches, as well C‐terminus are involved in sweetness brazzein. Second, charge plays an role...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProton-Translocating Carboxyl of Subunit c F1Fo H+-ATP Synthase: The Unique Environment Suggested by the pKa Determined 1H NMRFariba M. Assadi-Porter and Robert H. FillingameCite this: Biochemistry 1995, 34, 49, 16186–16193Publication Date (Print):December 12, 1995Publication History Published online1 May 2002Published inissue 12 December 1995https://pubs.acs.org/doi/10.1021/bi00049a034https://doi.org/10.1021/bi00049a034research-articleACS...
Obesity is a complex disease associated with environmental and genetic factors. 3-Iodothyronamine (T1AM) has revealed great potential as an effective weight loss drug. We used metabolomics transcriptional gene protein expression analysis to investigate the tissue specific metabolic reprogramming effects of subchronic T1AM treatment at two pharmacological daily doses (10 25 mg/kg) on targeted pathways. Multi-analytical results indicated that mg/kg can act novel master regulator both glucose...
Complex diseases such as polycystic ovary syndrome (PCOS) are associated with intricate pathophysiological, hormonal, and metabolic feedbacks that make their early diagnosis challenging, thus increasing the prevalence risks for obesity, cardiovascular, fatty liver diseases. To explore crosstalk between endocrine lipid pathways, we administered 3-iodothyronamine (T1AM), a natural analog of thyroid hormone, in mouse model PCOS analyzed plasma tissue extracts using multidisciplinary omics...
Polycystic ovary syndrome (PCOS) is associated with metabolic and endocrine disorders in women of reproductive age. The etiology PCOS still unknown. Mice prenatally treated glucocorticoids exhibit disturbances that are similar to those seen PCOS. We used an untargeted nuclear magnetic resonance (NMR)-based metabolomics approach understand the changes occurring plasma kidney over time female glucocorticoid-treated (GC-treated) mice. There significant amino acid levels (valine, tyrosine,...
We assessed adverse effects of external sublethal exposure Deepwater Horizon, Mississippi Canyon 252 oil on plasma and liver metabolome profiles the double-crested cormorant (Phalacrocorax auritus), a large (1.5 to 3.0 kg) diving waterbird common in Gulf Mexico. Metabolomics analysis avian showed significant negative metabolic profiles, some cases after only two exposures (26 g cumulative) oil. observed (p < 0.05) changes intermediate metabolites energy metabolism fatty acid amino pathways...
Brazzein (Brz) is a small (54 amino acid residue) sweet tasting protein with physical and taste properties superior to other non-carbohydrate sweeteners. In an investigation of sequence-dependent functional the protein, we used NMR spectroscopy determine three-dimensional structures dynamic two Brz variants: one single-site substitution (D40K), which three-fold sweeter than wild-type Brz, two-residue insertion between residues 18 19 (ins18 RI19 ), devoid sweetness. Although folds variants...
In sequence-function investigations, approaches are needed for rapidly screening protein variants possible changes in conformation. Recent NMR methods permit direct detection of hydrogen bonds through measurements scalar couplings that traverse (transhydrogen bond couplings). We have applied this approach to screen a series five single site mutants the sweet brazzein with altered sweetness backbone bonding respect wild-type. Long range, three-dimensional data correlating connectivities among...