A5086158740- Nitric Oxide and Endothelin Effects
- Antimicrobial Resistance in Staphylococcus
- Phosphodiesterase function and regulation
- Bacterial biofilms and quorum sensing
- Metalloenzymes and iron-sulfur proteins
- Wastewater Treatment and Nitrogen Removal
- Adenosine and Purinergic Signaling
- Cardiac Ischemia and Reperfusion
- Anaerobic Digestion and Biogas Production
- Enzyme Structure and Function
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Erythrocyte Function and Pathophysiology
- Chronic Kidney Disease and Diabetes
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- Diabetes Treatment and Management
- Hemoglobinopathies and Related Disorders
- Microbial Community Ecology and Physiology
- Blood Pressure and Hypertension Studies
- Microbial bioremediation and biosurfactants
- Complement system in diseases
- Esophageal and GI Pathology
Cyclerion (United States)
2020
Ironwood Pharmaceuticals (United States)
2014-2018
Cornell University
1994-2000
Rockefeller University
1998-2000
Tetrachloroethene is a prominent groundwater pollutant that can be reductively dechlorinated by mixed anaerobic microbial populations to the nontoxic product ethene. Strain 195, coccoid bacterium dechlorinates tetrachloroethene ethene, was isolated and characterized. Growth of strain 195 with H 2 as electron donor acceptor pair required extracts from cultures. resistant ampicillin vancomycin; its cell wall did not react peptidoglycan-specific lectin ultrastructure resembled S-layers Archaea....
The expression of many virulence determinants in<i>Staphylococcus aureus</i> including α-hemolysin-, protein A-, and fibronectin-binding proteins is controlled by global regulatory loci such as <i>sar</i> <i>agr</i>. In addition to controlling target gene via <i>agr</i>(<i>e.g.</i> α-hemolysin), the locus can also regulate transcription <i>agr</i>-independent mechanisms. particular, we have found that SarA, major encoded within <i>sar</i>, binds a conserved sequence, homologous SarA-binding...
The expression of many virulence determinants inStaphylococcus aureus is controlled by regulatory loci such as agr and sar. We have previously shown that the SarA protein required for optimal transcription RNAII RNAIII in locus. To define specific molecular interaction, we overexpressed a glutathioneS-transferase (GST) fusion cloning 372-base pair (bp) sarA gene into vector. purified GST-SarA well cleaved were able to bind specifically P2, P3, combined P2-P3 promoter fragments gel shift...
Fibronectin‐binding proteins (FnBPs) are thought to be important for the attachment of Staphylococcus aureus during infection. The regulation genes fnb A and B by global regulatory loci sar agr was examined using site‐specific mutants S. strain Newman. results from binding assays both aqueous solid‐phase fibronectin as well ligand blotting with biotinylated showed that expression FnBPA is enhanced in mutant but inhibited – double mutant. same pattern observed Northern blot analysis...
Determination of the nucleotide sequence nitrogenase structural genes (nifHDK2) from Methanosarcina barkeri 227 was completed in this study by cloning and sequencing a 2.7-kb BamHI fragment containing 3' end nifK2 1,390 bp nifE2-homologous genes. Open reading frame is 1,371 long including stop codon TAA encodes polypeptide 456 amino acids. Phylogenetic analysis deduced acid sequences nifE2 gene products M. showed that both cluster most closely with corresponding nif-1 Clostridium...
To evaluate the role of SigB in modulating expression virulence determinants Staphylococcus aureus, we constructed a sigB mutant RN6390, prototypic S. aureus strain. The mutation gene was confirmed by absence protein on an immunoblot as well failure to activate sigmaB-dependent promoters (e.g., sarC promoter) aureus. Phenotypic analysis indicated that both alpha-hemolysin level and fibrinogen-binding capacity were up-regulated strain compared with parental increase correlated enhanced...
The control of virulence determinant expression in Staphylococcus aureus is a complex process involving global regulatory loci such as sar and agr . locus consists 372 bp sarA open reading frame (ORF) preceded by triple promoter region interspersed with two putative smaller ORFs (ORF3 ORF4). system yields three overlapping transcripts ( A, C B 0.56, 0.8 1.2 kb respectively). We have recently shown that the SarA protein binds to stimulate transcription RNAII RNAIII, major encoded within...
L. Sibold, M. Henriquet, O. Possot, and J.-P. Aubert (Res. Microbiol. 142:5-12, 1991) cloned sequenced two nifH-homologous open reading frames (ORFs) from Methanosarcina barkeri 227. Phylogenetic analysis of the deduced amino acid sequences nifH ORFs showed that nifH1 clusters with genes alternative nitrogenases, while nifH2 gram-positive eubacterium Clostridium pasteurianum. The N-terminal sequence purified nitrogenase component 2 (the gene product) was identical predicted for nifH2, dot...
Impaired nitric oxide signaling through soluble guanylate cyclase has been implicated in the pathophysiology of diabetic kidney disease. Praliciguat, a stimulator that amplifies signaling, inhibited inflammation and fibrosis animal models.In phase 2 trial, 156 adults with type diabetes, eGFR 30-75 ml/min per 1.73 m2, urine albumin-creatinine ratio 200-5000 mg/g treated renin-angiotensin system inhibitors were randomly allocated 1:1:1 to placebo, 20 mg praliciguat, or 40 praliciguat daily for...
Nitric oxide (NO)-soluble guanylate cyclase (sGC)-cyclic 3',5' GMP (cGMP) signaling plays a central role in regulation of diverse processes including smooth muscle relaxation, inflammation, and fibrosis. sGC is activated by the short-lived physiologic mediator NO. stimulators are small-molecule compounds that directly bind to enhance NO-mediated cGMP signaling. Olinciguat,...
ABSTRACT Methanosarcina barkeri 227 possesses two clusters of genes potentially encoding nitrogenases. We have previously demonstrated that one cluster, called nif2 , is expressed under molybdenum (Mo)-sufficient conditions, and the deduced amino acid sequences for nitrogenase structural in cluster most closely resemble those Mo gram-positive eubacterium Clostridium pasteurianum . The cloned nifH1 from M. shows phylogenetic relationships with components eubacterial Mo-independent alternative...
ABSTRACT Transcription initiation in Archaea (archaebacteria) resembles the eucaryotic process, having been shown to involve TATA box-like promoter regions as well TATA-binding protein and TFIIB homologs. However, little is known about transcription regulation archaea. We have previously demonstrated that transcripts of nifHDK2 genes, encoding Methanosarcina barkeri nitrogenase, are present N 2 -grown cells but not ammonium-grown cells, indicating nif regulated by nitrogen source. In this...
Discovery of IWP-051, a novel orally bioavailable soluble guanylate cyclase stimulator with sustained and dose-dependent hemodynamic effects Takashi Nakai, Nicholas R Perl, Rajesh Iyengar, Ara Mermerian, G-Yoon J Im, Thomas W-H Lee, Glen Rennie, James Jia, Paul A Renhowe, Timothy C Barden, E Sheppeck II, Karthik Iyer, Joon Jung, G Todd Milne, Chrissie Segal, Kimberly Long, Joy Miyashiro, Sylvie Bernier, Sarah Jacobson, Jenny Tobin, Courtney Shea, Peter Germano, Yueh-tyng Chien, Daniel Zimmer