A5041525271- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Estrogen and related hormone effects
- Retinoids in leukemia and cellular processes
- Cancer Cells and Metastasis
- Fibroblast Growth Factor Research
- Hippo pathway signaling and YAP/TAZ
- Virus-based gene therapy research
- Protein Degradation and Inhibitors
- Cancer-related molecular mechanisms research
- DNA Repair Mechanisms
- Plant Virus Research Studies
- Skin and Cellular Biology Research
- Polyomavirus and related diseases
- Immune Cell Function and Interaction
- Plant Disease Resistance and Genetics
- T-cell and B-cell Immunology
- Cervical Cancer and HPV Research
- Genomics and Rare Diseases
- Cell Adhesion Molecules Research
- Muscle Physiology and Disorders
- RNA and protein synthesis mechanisms
Université de Toulouse
2008-2023
Université Toulouse III - Paul Sabatier
1998-2023
Centre National de la Recherche Scientifique
1996-2023
Laboratoire de Biologie Moléculaire des Eucaryotes
2008-2020
Institut de Pharmacologie et de Biologie Structurale
2003-2010
Bial (Portugal)
2010
Inserm
1996-2003
Institut de génétique et de biologie moléculaire et cellulaire
1998-2003
National Hellenic Research Foundation
2003
Collège de France
1996-1998
THAP1 is a sequence-specific DNA binding factor that regulates cell proliferation through modulation of target genes such as the cycle-specific gene RRM1. Mutations in domain, an atypical zinc finger (THAP-zf), have recently been found to cause DYT6 dystonia, neurological disease characterized by twisting movements and abnormal postures. In this study, we report shares sequence characteristics, vivo expression patterns protein partners with THAP3, another THAP-zf protein. Proteomic analyses...
Purpose Epithelial-Mesenchymal Transition (EMT) features appear to be key events in development and progression of breast cancer. Epigenetic modifications contribute the establishment maintenance cancer subclasses, as well EMT process. Whether histone variants these transformations is not known. We investigated relative expression levels macroH2A1 splice correlated it with status/prognosis/types. Methods To detect differential variant mRNAs cells tumor samples, we used following databases:...
ABSTRACT The histone variant macroH2A1.1 plays a role in cancer development and metastasis. To determine the underlying molecular mechanisms, we mapped genome-wide localization of endogenous human breast cell line MDA-MB-231. We demonstrate that specifically binds to active promoters enhancers addition facultative heterochromatin. Selective knock down deregulates expression hundreds highly genes. Depending on chromatin landscape, acts through two distinct mechanisms. first mitigates...
We have identified novel interactions between the human (h)TATA-binding protein-associated factor TAFII55 and ligand-binding domains (LBDs) of nuclear receptors for vitamin D3 (VDR) thyroid hormone (TRα). Following expression in Cos cells, hTAFII55 interacts with VDR TRα LBDs a ligand-independent manner whereas no retinoid X (RXRs) or other were observed. Deletion mapping indicates that 40-amino-acid region spanning α-helices H3 to H5 but not equivalent highly related RXRγ. also chimeric...
We have cloned and characterized the human TATA-binding protein (TBP)-associated factor hTAFII55. hTAFII55, which has no known Drosophila counterpart, is present in both of previously described TFIIDα TFIIDβ subpopulations. describe interactions hTAFII55 with other subunits transcription TFIID. By cotransfection COS cells, we show that interacts hTAFII250, hTAFII100, hTAFII28, hTAFII20, hTAFII18, but not hTAFII30 or TBP. Analysis binding TBP to hTAFII28 deletion mutants indicates distinct...
Human papillomavirus 8 (HPV8) is one of the oncogenic HPV types specifically associated with skin cancers epidermodysplasia verruciformis patients. The early gene products this virus exert functions in transformation (E2, E6, E7), replication (E1, E2) and control viral transcription E7). Many cellular transactivators have been shown to interact selectively directly a number TATA-box-binding protein (TBP)-associated factors (TAF<sub>II</sub>s), which then play role as...
c-Jun is a member of the AP-1 family transcription factors regulating expression specific target genes in variety cellular processes including proliferation, stress response, and tumorigenicity. In present study we have analyzed mechanism function as transactivator with respect to members basal machinery, TATA-binding protein-associated (TAFs). We show that one family, human TAF7 (formerly TAFII55), physically interacts through two independent interaction domains, within N- C-terminal part...
MacroH2A histone variants have functions in differentiation, somatic cell reprogramming and cancer. However, at present, it is not clear how macroH2As affect gene regulation to exert these functions. We parted from the initial observation that loss of total macroH2A1 led a change morphology murine myotubes differentiated ex vivo. The fusion myoblasts key process embryonic myogenesis highly relevant for muscle regeneration after acute or chronic injury. focused on this physiological process,...
Fibroblast growth factor 2 (FGF-2) has been detected in the nuclei of many tissues and cell lines. Here we demonstrate that FGF-2 added exogenously to NIH3T3 cells enters nucleus interacts with nuclear active 90-kDa ribosomal S6 kinase (RSK2) a cycle-dependent manner. By using purified proteins, is shown directly interact through two separate domains RSK2 on both sides hydrophobic motif, namely NH2-terminal domain (residues 360-381) by amino acid Ser-117 COOH-terminal 388-400) acids Leu-127...
Using coexpression in COS cells, we have identified novel interactions between the human TATA-binding protein-associated factor 28 (hTAF(II)28) component of transcription IID and ligand binding domains (LBDs) nuclear receptors for vitamin D3 (VDR) thyroid hormone (TRalpha). Interaction hTAF(II)28 VDR TR LBDs was ligand-reversible, whereas no retinoid X (RXRs) or other were observed. TAF(II)28 interacted with two regions VDR, a 40-amino acid region spanning alpha-helices H3-H5 alpha-helix H8....
Coexpression of the human TATA-binding protein (TBP)-associated factor 28 (hTAFII28) with altered-specificity mutant TBP spm3 synergistically enhances transcriptional activation by function 2 nuclear receptors (NRs) for estrogen and vitamin D3 from a reporter plasmid containing TGTA element in mammalian cells. This synergy is abolished mutation specific amino acids alpha2-helix histone fold conserved C-terminal region hTAFII28. Critical are found on both exposed hydrophilic face this helix...
Heterochromatin formation plays a pivotal role in regulating chromatin organization and influences nuclear architecture, genome stability expression. Among the regions at which heterochromatin is present, pericentric are known to recruit histone variant macroH2A1. However, factors mechanisms behind macroH2A1 incorporation into these have not been explored. In this study, we probe different conditions that lead recruitment of pericentromeric elucidate its underlying functions. Through...
Heterochromatin formation plays a pivotal role in regulating chromatin organization and influences nuclear architecture genome stability expression. Amongst the locations where heterochromatin is found, pericentric regions have capability to attract histone variant macroH2A1. However, factors mechanisms behind macroH2A1 incorporation into these not been explored. In this study, we probe different conditions that lead recruitment of pericentromeric elucidate its underlying functions. Through...
Abstract The histone variant macroH2A1.1 (mH2A1.1) plays a role in cancer development and metastasis-related processes. To determine the underlying molecular mechanisms, we mapped genome-wide localization of endogenous mH2A1.1 human breast cell MDA-MB 231. We demonstrate that specifically binds to active promoters enhancers addition facultative heterochromatin. Selective knock-down deregulates expression hundreds highly genes. Depending on chromatin landscape, acts through two distinct...
La microscopie intravitale est une technique de développement récent qui permet visualiser des phénomènes <i>in situ<i/> directement chez l’animal vivant. Elle d’enregistrer, par l’intermédiaire d’un microscope adapté, fluorescents, cinétiques, dans l’espace et le temps, apparaissant à l’échelle tissu ou organe un animal intact, éveillé endormi. Appliquée au cancer, elle a surtout été utilisée l’étude la vascularisation intratumorale. Cependant, tentatives récentes isolées laissent présager...