A5041651792- Developmental Biology and Gene Regulation
- Hemispheric Asymmetry in Neuroscience
- Cellular Mechanics and Interactions
- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Morphological variations and asymmetry
- Lymphatic System and Diseases
- Evolutionary Psychology and Human Behavior
- Axon Guidance and Neuronal Signaling
- Animal Behavior and Reproduction
- Nuclear Structure and Function
- Retinoids in leukemia and cellular processes
- Craniofacial Disorders and Treatments
- Nerve injury and regeneration
- Chemokine receptors and signaling
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Plant Disease Resistance and Genetics
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- IL-33, ST2, and ILC Pathways
Centre de Biologie du Développement
2006-2023
Université de Toulouse
2009-2023
Centre National de la Recherche Scientifique
2003-2023
Université Toulouse III - Paul Sabatier
2007-2023
University College London
2009-2014
Institut de Pharmacologie et de Biologie Structurale
2003-2010
University of Bristol
2010
Inserm
2000
We have recently described an evolutionarily conserved protein motif, designated the THAP domain, which defines a previously uncharacterized family of cellular factors (THAP proteins). The domain exhibits similarities to site-specific DNA-binding Drosophila P element transposase, including putative metal-coordinating C2CH signature (CX 2–4 CX 35–53 2 H). In this article, we report comprehensive list ≈100 distinct proteins in model animal organisms, human nuclear proapoptotic THAP1 and...
The habenulae are evolutionarily conserved bilateral nuclei in the epithalamus that relay input from forebrain to ventral midbrain. In zebrafish, display left-right (L/R) asymmetries gene expression and axonal projections. elaboration of habenular requires presence a second asymmetric structure, parapineal, laterality which is biased by unilateral Nodal signalling. Here we show neurons present earlier left habenula than right, but, contrast other asymmetry phenotypes, this neurogenesis not...
Abstract With the ultimate goal of understanding how genetic modules have evolved in telencephalon, we set out to modernize functional analysis cross‐species cis‐regulatory elements mouse. In utero electroporation is rapidly replacing transgenesis as method choice for gain‐ and loss‐of‐function studies murine but application this technique transcriptional regulation has yet be fully explored exploited. To empirically define developmental stages required target specific populations neurons...
Although cell-to-cell heterogeneity in gene and protein expression within cell populations has been widely documented, we know little about its biological functions. By studying progenitors of the posterior region bird embryos, found that levels transcription factors Sox2 Bra, respectively involved neural tube (NT) mesoderm specification, display a high degree heterogeneity. combining forced downregulation approaches with time-lapse imaging, demonstrate Sox2-to-Bra ratio guides progenitor’s...
Five fibroblast growth factor 2 (FGF-2) isoforms are synthesized from human FGF-2 mRNA by a process of alternative initiation translation. The regulation isoform expression the 5823-nucleotide-long 3′-untranslated region containing eight polyadenylation sites was examined. Because previous studies had shown that regulated in primary cells but not transformed cells, skin fibroblasts were used this study. Using an approach cell transfection with synthetic reporter mRNAs, novel translational...
Cancer and dendritic cells recognize migrate toward chemokines secreted from lymphatics use this mechanism to invade the lymphatic system, cancer metastasize through it. The lymphatic-secreted chemokine ligand CCL21 has been identified as a key regulatory molecule in switch metastatic phenotype melanoma breast cells. However, it is not known whether inhibition potential therapeutic strategy for of metastasis. Here, we describe an engineered CCL21-soluble inhibitor, Chemotrap-1, which...
Left-right (L/R) asymmetries in the brain are thought to underlie lateralised cognitive functions. Understanding how neuroanatomical established has been achieved through study of zebrafish epithalamus. Morphological symmetry epithalamus is broken by leftward migration parapineal, which required for subsequent elaboration left habenular identity; nuclei flank midline and show L/R marker expression connectivity. The Nodal target pitx2c expressed epithalamus, but nothing known about its role...
Significance The ability of cells to migrate collectively underlies many biological processes. parapineal is a small group that requires Fgf8 from the midline left side zebrafish forebrain. Studying dynamics FGF pathway activation reveals activity restricted few left-sided cells. Global interferes with migration in wild-type embryos, while focal can restore fgf8 −/− mutants, indicating leading required for collective migration. We show influenced by Nodal signaling. Our findings may apply...
The habenulae are highly conserved nuclei in the dorsal diencephalon that connect forebrain to midbrain and hindbrain. These have been implicated a broad variety of behaviours humans, primates, rodents zebrafish. Despite this, molecular mechanisms control genesis differentiation neural progenitors remain relatively unknown. We previously shown that, zebrafish, timing habenular neurogenesis is left-right asymmetric absence Nodal signalling this asymmetry lost. Here, we show requires homeobox...
High levels of within-population behavioural variation can have drastic demographic consequences, thus changing the evolutionary fate populations. A major source heterogeneity is personality. Nonetheless, it still relatively rarely accounted for in social learning studies that constitute most basic process cultural transmission. Here, we performed female mosquitofish ( Gambusia holbrooki ) a experiment context mate choice, situation called copying (MC), and which there strong evidence lead...
Coordinated migration of cell collectives is important during embryonic development and relies on cells integrating multiple mechanical chemical cues. Recently, we described that focal activation the FGF pathway promotes parapineal in zebrafish epithalamus. How activity restricted to leading this system is, however, unclear. Here, address role Notch signaling modulating within parapineal. While loss-of-function results an increased number activating pathway, global decreases it; both...
The zebrafish Danio rerio is an important model organism, but little known about its mating preferences and how these are influenced by personality traits like boldness. In this study, we tested two strains of addressed whether females used social information to build a preference, behavior called mate copying, learning was affected Thus, provided positive for small males test female changed their preference after observing pair large male with demonstrator next the one. After that, observer...
Abstract Morphogenesis of vertebrate embryos requires extracellular matrix (ECM) in multiple developmental contexts 1 . Symmetry-breaking events initially homogenous tissues trigger collective cellular dynamics which follow fluid-like behaviors, and the ECM, is constant engagement with morphing tissues, also undergoes motion 2–4 Cell ECM deformations require coordination, however interplay between them at tissue-scale remains largely unexplored. Here, we reveal a novel mechanism coupling...
Abstract Coordinated migration of cell collectives is important during embryonic development and relies on cells integrating multiple mechanical chemical cues. Recently, we described that focal activation the FGF pathway promotes parapineal in zebrafish epithalamus. How activity restricted to leading this system is, however, unclear. Here, address role Notch signaling modulating within parapineal. While loss-of-function results an increased number activating pathway, global decreases it;...