A5029643983- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- interferon and immune responses
- Pulmonary Hypertension Research and Treatments
- 3D Printing in Biomedical Research
- Neuroblastoma Research and Treatments
- Inhalation and Respiratory Drug Delivery
- Systemic Sclerosis and Related Diseases
- Advanced Biosensing Techniques and Applications
- bioluminescence and chemiluminescence research
- Bioinformatics and Genomic Networks
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Viral Infectious Diseases and Gene Expression in Insects
- Chromosomal and Genetic Variations
- Cell Image Analysis Techniques
- Histone Deacetylase Inhibitors Research
- Microfluidic and Bio-sensing Technologies
- Peroxisome Proliferator-Activated Receptors
- Dermatologic Treatments and Research
- Cellular Mechanics and Interactions
- Nuclear Structure and Function
Newcastle University
2015-2023
Smiths Detection (France)
1990
Bacterial infections after lung transplantation cause airway epithelial injury and are associated with an increased risk of developing bronchiolitis obliterans syndrome. The damaged epithelium is a source alarmins that activate the innate immune system, yet their ability to fibroblasts in development syndrome has not been evaluated. Two were measured longitudinally bronchoalveolar lavages from transplant recipients who developed compared stable controls. In addition, conditioned media human...
Abstract There are thousands of known cellular phosphorylation sites, but the paucity ways to identify kinases for particular events remains a major roadblock understanding kinase signaling. To address this, we here develop generally applicable method that exploits large number inhibitors have been profiled on near-kinome-wide panels protein kinases. The inhibition profile each provides fingerprint allows identification unknown acting target phosphosites in cell extracts. We validate diverse...
Abstract Protein kinases that phosphorylate histones are ideally-placed to influence the behavior of chromosomes during cell division. Indeed, a number conserved histone phosphorylation events occur prominently mitosis and meiosis in most eukaryotes, including on H3 at threonine-3 (H3T3ph). At least two kinases, Haspin VRK1 (NHK-1/ballchen Drosophila ), have been proposed carry out this modification. Phosphorylation by has defined roles mitosis, but significance activity towards dividing...
The connection between cells and their substrate is essential for biological processes such as cell migration. Atomic force microscopy nanoindentation has often been adopted to measure single-cell mechanics. Very recently, fluidic developed enable rapid measurements of adhesion. However, simultaneous characterization the cell-to-material adhesion viscoelastic properties same challenging. In this study, we present a new approach simultaneously determine these single cells, using microscopy....
NF-κB regulates genes that control inflammation, cell proliferation, differentiation and survival. Dysregulated signalling alters normal skin physiology deletion of cRel limits bleomycin-induced fibrosis. This study investigates the role in modulating fibroblast phenotype context SSc.Fibrosis was assessed histologically mice challenged with bleomycin to induce lung or RNA sequencing pathway analysis performed on wild type Rel-/- murine dermal fibroblasts. Functional assays examined migration...
Bioluminescence assays using luciferase enzymes are widely used in research to monitor gene expression and an array of other cell properties, split can be measure protein interactions biochemical living cells. When these methods employed chemical library screening efforts, it is vital that the activity enzyme itself not strongly influenced by components. Here, we developed a NanoBiT assay phosphorylation Histone H3 peptides test robustness interference from two libraries commonly kinase...
Abstract Histone modifications influence the recruitment of reader proteins to chromosomes regulate events including transcription and cell division. The idea a histone code, where combinations specify unique downstream functions, is widely accepted can be demonstrated in vitro. For example, on synthetic peptides, phosphorylation H3 at threonine-3 (H3T3ph) prevents binding that recognize trimethylation adjacent lysine-4 (H3K4me3), TAF3 component TFIID. To study these combinatorial effects...
ABSTRACT Histone modifications influence the recruitment of reader proteins to chromosomes regulate events including transcription and cell division. The idea a histone code, where particular combinations specify unique downstream functions, is widely accepted can be demonstrated in vitro . For example, on synthetic peptides, phosphorylation H3 at threonine-3 (H3T3ph) prevents binding that recognise trimethylation adjacent lysine-4 (H3K4me3), TAF3 component TFIID. To study these...
Abstract Protein kinases that phosphorylate histones are ideally-placed to influence the behavior of chromosomes during cell division. Indeed, a number conserved histone phosphorylation events occur prominently mitosis and meiosis in most eukaryotes, including on H3 at threonine-3 (H3T3ph). At least two kinases, Haspin VRK1 (NHK-1/ballchen Drosophila ), have been proposed carry out this modification. Phosphorylation by has defined roles mitosis, but significance activity towards dividing...