A5043560581- Angiogenesis and VEGF in Cancer
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Cancer-related gene regulation
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Heat shock proteins research
- thermodynamics and calorimetric analyses
- Immune cells in cancer
- MicroRNA in disease regulation
- Cancer Treatment and Pharmacology
- Protease and Inhibitor Mechanisms
- Cancer, Lipids, and Metabolism
- Glycosylation and Glycoproteins Research
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Proteoglycans and glycosaminoglycans research
- Genital Health and Disease
- Innovative Microfluidic and Catalytic Techniques Innovation
- ATP Synthase and ATPases Research
- Circular RNAs in diseases
- Lung Cancer Treatments and Mutations
- TGF-β signaling in diseases
Centre Oscar Lambret
2011-2023
Centre National de la Recherche Scientifique
2013-2023
Inserm
1996-2023
Laboratory for Integrated Micro-Mechatronic Systems
2019-2023
Tokyo University of Science
2021-2023
The University of Tokyo
2019-2023
Mécanismes de Tumorigenèse et Thérapies Ciblées
2009-2022
Université de Lille
2006-2022
Meguro Parasitological Museum
2022
Tokyo Metropolitan Komaba High School
2022
Mammalian heat shock genes are regulated at the transcriptional level by factor-1 (HSF-1), a sequence-specific transcription factor. We have examined role of serine phosphorylation HSF-1 in regulation gene transcription. Our experiments show that mitogen-activated protein kinases (MAPKs) ERK-1 family phosphorylate on residues and repress activation 70B (HSP70B) promoter vivo. These effects MAPK transmitted through specific residue (Ser-303) located proline-rich sequence within regulatory...
Malignant melanoma cells are known to have altered expression of growth factors compared with normal human melanocytes. These changes probably favor tumor and progression influence the environment. The induction transforming factor beta1 (TGF-beta1), TGF-beta2, TGF-beta3 in malignant has been reported before, whereas related bone morphogenic protein (BMP) molecules not analyzed melanomas until now. Here, we show that BMP4 BMP7 up-regulated nine cell lines, BMP2 is overexpressed only two...
Rev-Erbα (NR1D1) is an orphan nuclear receptor encoded on the opposite strand of thyroid α gene. mRNA induced during adipocyte differentiation 3T3-L1 cells, and its expression abundant in rat adipose tissue. Peroxisome proliferator-activated γ (PPARγ) (NR1C3) a controlling insulin sensitivity. Here we show that by PPARγ activation with rosiglitazone epididymal perirenal tissues vivo as well adipocytes vitro. Furthermore, activated induces promoter activity binding to direct repeat (DR)-2...
Angiogenesis is the formation of new capillaries from pre-existing blood vessels and participates in proper vasculature development. In pathological conditions such as cancer, abnormal angiogenesis takes place. primarily carried out by endothelial cells, innermost layer vessels. The vascular growth factor-A (VEGF-A) its receptor-2 (VEGFR-2) trigger most mechanisms activating regulating angiogenesis, have been targets for development drugs. However, experimental assays assessing rely on...
Inactivation of the tumor suppressor gene RASSF1A by promoter hypermethylation represents a key event underlying initiation and progression lung cancer. inactivation is also associated with poor prognosis may promote metastatic spread. In this study, we investigated how conferred invasive phenotypes to human bronchial cells. RNAi-mediated silencing induced epithelial-to-mesenchymal transition (EMT), fomenting motile cellular phenotype in vitro increased prowess vivo. Mechanistic...
Cytokines interact with their receptors in the extracellular space to control immune responses. How physicochemical properties of influence cytokine signaling is incompletely elucidated. Here, we show that activity interleukin-2 (IL-2), a critical T cell immunity, profoundly affected by pH, limiting IL-2 within acidic environment tumors. Generation lactic acid tumors limits STAT5 activation, effector differentiation, and antitumor immunity CD8
Heat shock factor 1 (HSF1) is the key transcriptional regulator of heat genes that protect cells from environmental stress. However, because gene expression deleterious to growth and development, we have examined mechanisms for HSF1 repression at temperatures, focusing on role phosphorylation. Mitogen-activated protein kinases (MAPKs) ERK family phosphorylate represses function. The mechanism involves initial phosphorylation by MAP kinase serine 307, which primes secondary glycogen synthase...
Specific binding of angiogenin (ANG) to calf pulmonary artery endothelial cells was demonstrated. Cellular at 4 degrees C 125I-labeled human recombinant ANG time and concentration dependent, reversible, saturable in the presence increasing amounts unlabeled molecules. The interaction shown be specific since a large excess reduced labeled by 80%, whereas similar doses RNase A, structurally related protein, had no effect. Scatchard analyses data revealed two apparent components. High-affinity...
Downregulating the leukocyte adhesion molecules expressed by endothelial cells that line tumor blood vessels can limit entry of immune effector into mass, thereby contributing to tumoral escape. Egfl7 (also known as VE-statin) is a secreted protein specifically in normal tissues and cancer various human tumors. High levels correlate with higher grade poorer prognosis. Here we show expression breast lung carcinoma accelerates growth metastasis immunocompetent mice but not immunodeficient...
Presence in glioblastomas of cancer cells with normal neural stem cell (NSC) properties, tumor initiating capacity, and resistance to current therapies suggests that glioblastoma stem-like (GSCs) play central roles development. We cultured human GSCs endowed all features cells, including initiation after xenograft radio-chemoresistance. established proteomes from four GSC cultures their corresponding whole tissues (TTs) NSCs. Two-dimensional difference gel electrophoresis tandem mass...
Heat shock factor 2 (HSF2) belongs to a family of structurally related transcription factors, which share the property binding heat elements in promoters hsp molecular chaperone genes. However, unlike HSF1, is essential for gene transcription, cellular functions HSF2 are not well known. Here we show that human HSF2, although an ineffective activator hsp70 promoter vitro and vivo absence stress, participates activation by shock. was not, however, activated cells deficient functional...
When coated on bacteriological plastic at doses greater than or equal to 0.1 microgram/cm2, human and bovine angiogenin support calf pulmonary artery endothelial Chinese hamster fibroblast cell adhesion spreading, but do not affect when in solution. The kinetics of attachment are indistinguishable from those the presence gelatin. Calcium and/or magnesium ions critical for spreading onto protein synthesis glycoprotein secretion necessary. Adhesion is altered by addition incubation solution...
AimsmiR126-5p is processed from the miR126-3p/-5p duplex, which expressed in endothelial cells and gives rise to guide strand miR126-3p passenger miR126-5p. has prominent roles vascular development diseases, whereas expression physiological functions of miR126-5p are unknown. The purpose this study was evaluate role blood vessel cells.
Vaccines designed to abrogate the tolerance of tumor self-antigens and amplify cytotoxic CD8+ T cells (CTLs) have promise for treatment cancer. Type I natural killer (NKT) attracted considerable interest in cancer therapy field. In current study, we exploited unique ability NKT serve as T-helper license dendritic (DCs) cross priming with aim generate efficient CTL antitumor responses. To this end, a nanoparticle-based vaccine target cross-priming DCs via Clec9a endocytic pathway. Our results...
ABSTRACT MicroRNAs are key regulators of angiogenesis, as illustrated by the vascular defects observed in miR-126-deficient animals. The miR-126 duplex gives rise to two mature microRNAs (miR-126-3p and -5p). these mutant animals were attributed loss miR-126-3p but role miR-126-5p during normal angiogenesis vivo remains unknown. Here, we show that is expressed endothelial cells also retinal ganglion (RGCs) mouse postnatal retina participates protecting from apoptosis establishment...