BY PINA COLARUSSO, LINDA H. KIDDER, IRA W. LEVIN, JAMES C. FRASER, JOHN F. ARENS, AND E. NEIL LEWIS* LABORATORY OF CHEMICAL PHYSICS, NATIONAL INSTITUTE DIABETES DIGESTIVE KIDNEY DISEASES, THE INSTITUTES HEALTH, BETHESDA, MARYLAND 20892 (P.C., L.H.K., I.W.L., E.N.L.); VANGUARD RESEARCH, INC., FAIRFAX, VIRGINIA 22030 (J.C.F.); SPACE SCIENCES LABORATORY, UNIVERSITY CALIFORNIA, BERKELEY, CALIFORNIA 94720 (J.F.A.)

To test the hypothesis that Fourier transform infrared (FTIR) spectral imaging, coupled with multivariate data processing techniques, can image spatial distribution of matrix constituents in native and engineered cartilage samples.Tissue sections from trypsin-digested bovine nasal (BNC) cartilage, generated by chick sternal chondrocytes grown a hollow fiber bioreactor, were placed either on calcium fluoride windows for FTIR analysis or gelatinized microscope slides histologic analysis. Based...

A combination of Fourier transform infrared (FT-IR) spectroscopy and microscopy, FT-IR microspectroscopy, has been used to characterize sections human colorectal adenocarcinoma. In this report, a database 2601 high quality point spectra from 26 patient samples seven different histological structures was recorded analyzed. The computer-based analysis the IR carried out in four steps: (1) an initial test for spectral quality, (2) data pre-processing, (3) reduction feature selection, (4)...

By combining step-scan Fourier-transform Michelson interferometry, an infrared microscope, and mercury cadmium telluride focal-plane array image detection we have constructed a mid-infrared spectroscopic imaging system that simultaneously records high-fidelity images spectra of materials from 3500 to 900 cm-1 (2.8 11 µm) at variety spectral resolutions. The fidelity the is determined by pixel number density array. Step-scan principles instrument design details are outlined. Spatial...

Determination of the physicochemical properties protein therapeutics and their aggregates is critical for developing formulations that enhance product efficacy, stability, safety manufacturability. Analytical challenges are compounded materials: (1) formulated at high concentration, (2) with a variety excipients, (3) available only in small volumes. In this article, new instrument described measures secondary tertiary structure, as well molecular size, over range concentrations formulation...

Cluster analysis and artificial neural networks (ANNs) are applied to the automated assessment of disease state in Fourier transform infrared microscopic imaging measurements normal carcinomatous immortalized human breast cell lines. K-means clustering is used implement an algorithm for assignment pixels image non-cell categories. Cell subsequently classified into carcinoma categories through use a feed-forward ANN computed with Broyden-Fletcher-Goldfarb-Shanno training algorithm. Inputs...

We have applied Fourier transform infrared (IR) spectroscopic imaging to the investigation of neuropathologic effects a genetic lipid storage disease, Niemann-Pick type C (NPC). Tissue sections both from cerebella strain BALB/c mice that demonstrated morphology and pathology human disease control animals were used. These samples analyzed by standard histopathological procedures as well this new IR approach. The absorbance images exhibit contrast based on biochemical variations allow for...

We describe a Raman imaging microscope that produces high‐fidelity, large format images and spectra from samples as small 1 μm in size. Laser illumination is delivered to the object by means of an infinity corrected objective, either galvanometer scanning system or widefield fibre optic. Wavelength selection scattered emission achieved acousto‐optic tunable filter (AOTF), which maintains image fidelity provides continuous random wavelength selection. The collimated AOTF output imaged first...

An instrument is described that simultaneously records images and spectra of materials in the infrared fingerprint region using a long-wavelength focal-plane array detector, step-scan Michelson interferometer, an microscope. With combination Fourier transform (FT) interferometry arsenic-doped silicon (Si: As) image detection, spectroscopic imaging system has been constructed maintains both instrumental multiplex multichannel advantage operates from approximately 4000 to 400 cm −1 . this...

Background: Phytocannabinoids naturally occur in the cannabis plant (Cannabis sativa), and Δ9-tetrahydrocannabinol (THC) cannabidiol (CBD) predominate. There is a need for rapid inexpensive methods to quantify total THC (for statutory definition) THC-CBD ratio classification into three chemotypes). This study explores capabilities of spectroscopic technique that combines ultraviolet-visible fluorescence, absorbance-transmittance excitation emission matrix (A-TEEM). Methods: The A-TEEM...

Abstract Historically near-infrared (NIR) spectroscopy has enjoyed significant success as the industrial ‘cousin’ of mid-infrared FTIR spectrometer. Most NIR spectrometers are significantly more rugged than their MIR counterparts, and therefore suited for use a QA/QC tool. Sample preparation is trivial or non-existent most solid-state liquid samples data collection rapid, while still achieving excellent signal to noise. All these factors combine produce an analytical measurement technology...

Rapidity of data acquisition, high image fidelity and large field view are tremendous value when looking for chemical contaminants or the proverbial "needle in haystack" - this case foreign inclusions histologic sections biopsy autopsy tissues. Near infrared imaging is one three techniques (NIR, MIR Raman) based on vibrational spectroscopy, provides distinct technical advantages application.We have chosen to utilize evaluate near (NIR) studies materials tissue because experimental...