In this review, we attempt to give a concise overview of recent progress made in mechanistic understanding protein aggregation, particulate formation and solution rheology. Recent advances analytical techniques methods for characterizing aggregation the formed particles as well advancements, technique limitations controversies field rheology are discussed. The focus review is primarily on biotherapeutics proteins/antibodies that relevant area. As per remit Current Opinion Colloid Interface...

High-concentration monoclonal antibody (mAb) formulations are frequently constrained by elevated viscosity and colloidal instability, stemming from enhanced intermolecular interactions under crowded conditions. This study delineates the thermodynamic rheological consequences of modulating protein–protein through excipient-mediated temperature-dependent mechanisms. Using an orthogonal analytical framework comprising diffusion interaction parameter ( k D) measurements, high-shear rheometry,...

The Brownian motion of colloidal spheres in aqueous poly(ethylene oxide) (PEO) solutions ranging concentration from 0.2 to 15 wt % is measured with diffusing wave spectroscopy over more than 6 decades time. sphere mean-squared displacements are shown satisfy the generalized Stokes−Einstein relation that relates particle displacement polymer solution shear modulus as microrheological creep compliance calculated was found agree by mechanical rheometry. zero viscosity dependence for entangled...

We present an investigation on the effect of inter-micellar connections (branches) rheology wormlike micelles. The system chosen is made lecithin, minute amounts water and organic solvents. Lecithin self-assemble into reverse micelles that can be branched or unbranched depending oil composition (and content). In this respect, cyclohexane favours disconnected while isooctane promotes formation branches. By using mixtures as phase different water/lecithin ratios, branch density finely tuned....

Determination of the physicochemical properties protein therapeutics and their aggregates is critical for developing formulations that enhance product efficacy, stability, safety manufacturability. Analytical challenges are compounded materials: (1) formulated at high concentration, (2) with a variety excipients, (3) available only in small volumes. In this article, new instrument described measures secondary tertiary structure, as well molecular size, over range concentrations formulation...

The viscoelastic properties of associative polymers are important not only for their use as rheology modifiers but also to understand complex structure in aqueous media. In this study, the dynamics comblike hydrophobically modified alkali swellable (HASE) probed using diffusing wave spectroscopy (DWS) based tracer microrheology. DWS-based microrheology accurately probes HASE polymers, and extracted microrheological moduli versus frequency profile obtained from technique closely matches that...

Epidermal growth factors (EGF) serve as promising candidates for skin regeneration and rejuvenation products, but their instability hinders them from widespread use. Protective immobilization directed release can be achieved through implementing a hydrogel delivery system. Alginate methylcellulose are both natural polymers offering biocompatibility environmental sensitivity. This blended gel system was investigated rheologically to understand its performance in topical applications. were...

Abstract Objective The main objective of this paper is to understand the rheological impact rhamnolipids biosurfactant (mono/dirhamnolipids mixture, CCB ) on a common personal care mixed surfactants system: anionic sodium laureth sulfate ( SLES and zwitterionic cocamidopropyl betaine CAPB ). ternary biosurfactant/surfactants mixtures were evaluated at three different formulation conditions. experimental results can provide guideline when applying in cosmetics products. Methods Traditional...

Hydrogels of biopolymers such as agar and gelatin are widely used in many applications, cases, the gels loaded with nanoparticles. The polymer chains these cross-linked by physical bonds into three-dimensional networks, mesh size networks typically being 10–100 nm. One class "soft" nanoparticles liposomes, which have an aqueous core surrounded a lipid bilayer. Solutes encapsulated liposomal can be delivered externally over time. In this paper, we create liposomes diameters ∼150 nm from...

The main objective of this study was to design a sustainable lip gloss from water-in-oil emulsions by understanding the rheology combining biosurfactants (rhamnolipids and sophorolipids) with silica particles (Aerosil powders). different parameters consisted particle diameter size, concentration within formulation, alongside types biosurfactant oils used. experimental results could provide information on formulation cosmetic such as products foundations.One parameter varied each time, while...

The purpose of this study is to understand the impact cationic polymer merquat on rheological behavior mixed surfactant system sodium lauryl ether sulfate (SLES) and cocamidopropyl betaine (CapB) as well varying formulation conditions wet lubrication performance SLES-CapB-Merquat system.Rotation mechanical Rheometry was used response systems. Frequency sweeps were conducted analyze properties at low frequency ranges bulk viscosity studied high shear rates salt concentrations. Wet combing...

The correlation between molecular changes and microstructural evolution of rheological properties has been demonstrated for the first time in a mixed anionic/zwitterionic surfactant-based wormlike micellar system. Utilizing novel combination DLS-microrheology Raman Spectroscopy, effect electrostatic screening on these anionic (SLES) zwitterionic (CapB) surfactant mixtures was studied by modulating NaCl concentration. As Spectroscopy delivers information about structure characterizes...

Non-native protein aggregation may occur during manufacturing and storage of therapeutics, this decrease drug efficacy or jeopardize patient safety. From a regulatory perspective, changes in higher order structure due to are particular interest but can be difficult monitor directly at elevated concentrations. The present report focuses on non-native antistreptavidin (AS) IgG1 30 mg/mL under solution conditions that prior work dilute concentrations (e.g., 1 mg/mL) indicated would result...

The main objective of this paper is to analyse and attempt understand the nature rheological changes observed dynamics Carbopol NF 980 hydroalcoholic gels neutralized specifically by triethanol amine (TEA), both as a function time alcohol type probe stabilities ageing effects in such carbopol gel systems. 3 systems were observed; included water-based, ethanol-based, isopropyl alcohol-based gel. It hoped that study shall shed light on dynamical microstructural evolution networked systems,...

Tracer particle microrheology using diffusing wave spectroscopy-based is demonstrated to be a useful method study the dynamics of aqueous Pluronic™ F108 solutions, which are viewed as solutions repulsive soft spheres. The measured zero-shear microviscosity noncrystallizing micellar dispersions indicates micelle corona dehydration upon increasing temperature. Colloidal sphere thermal motion shown exquisitely sensitive onset crystallization in these dispersions. High temperature dominated by...