A5056963168- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Biosimilars and Bioanalytical Methods
- Microfluidic and Bio-sensing Technologies
- Protein Structure and Dynamics
- Analytical Chemistry and Chromatography
- Musicology and Musical Analysis
- Glycosylation and Glycoproteins Research
- Historical Influence and Diplomacy
- Cancer-related cognitive impairment studies
- Classical Antiquity Studies
- Circadian rhythm and melatonin
- Membrane Separation and Gas Transport
- Renaissance and Early Modern Studies
- Sleep and Wakefulness Research
- Linguistic Studies and Language Acquisition
- Blood properties and coagulation
- Proteins in Food Systems
- Hemoglobin structure and function
- Erythrocyte Function and Pathophysiology
- Brain Metastases and Treatment
- Transgenic Plants and Applications
- Wireless Communication Networks Research
- Mass Spectrometry Techniques and Applications
Bristol-Myers Squibb (United States)
2017-2019
Amgen (United States)
2019
University of Delaware
2014-2017
Yale University
2014
Brown University
2014
Research Experiences for Undergraduates
2010
Lehigh University
2010
Zeolitic imidazolate frameworks (ZIFs) are hybrid organic-inorganic microporous materials that exhibit zeolite-like structures and can be synthesized with a wide range of pore sizes chemical functionality. ZIFs as thin films membranes interest for their applications in sensors gas separation. Here, we report method ZIF film membrane fabrication, based on support surface modification situ solvothermal growth, which has potential general application to other membranes. Our simple results...
In this review, we attempt to give a concise overview of recent progress made in mechanistic understanding protein aggregation, particulate formation and solution rheology. Recent advances analytical techniques methods for characterizing aggregation the formed particles as well advancements, technique limitations controversies field rheology are discussed. The focus review is primarily on biotherapeutics proteins/antibodies that relevant area. As per remit Current Opinion Colloid Interface...
Non-native protein aggregation is common in the biopharmaceutical industry and potentially jeopardizes product shelf life, therapeutic efficacy, patient safety. The present article focuses on relationship(s) among protein-protein interactions, aggregate growth mechanisms, morphologies, specific-ion effects for an anti-streptavidin (AS) immunoglobulin gamma 1 (IgG1). Aggregation mechanisms of AS-IgG1 were determined as a function pH NaCl concentration with sodium acetate buffer compared to...
Preferential interactions of proteins with water and osmolytes play a major role in controlling the thermodynamics protein solutions. While changes stability shifts phase behavior are often reported addition osmolytes, underlying and/or typically inferred rather than measured directly. In this work, Kirkwood–Buff integrals for protein–water (G12) protein–osmolyte (G23) were determined as function osmolyte concentration from density measurements antistreptavidin immunoglobulin gamma-1...
Monoclonal antibodies must be both chemically and physically stable to developed into safe effective drugs. Although there has been considerable progress in separately understanding the molecular determinants of antibody chemical physical stability, it remains poorly understood how defects one property (e.g., stability) impact other stability). Here, we have investigated a common modification (deamidation) on stability two monoclonal as function pH (from 3.8 7.4). Interestingly, find that...
While forgetfulness is widely reported by breast cancer survivors, studies documenting objective memory performance yield mixed, largely inconsistent, results. Failure to find consistent, issues may be due the possibility that survivors misattribute their experience of primary rather than difficulties in attention at time learning. To clarify potential issues, factor scores for Attention Span, Learning Efficiency, Delayed Memory, and Inaccurate Memory were analyzed California Verbal...
Non-native protein aggregation may occur during manufacturing and storage of therapeutics, this decrease drug efficacy or jeopardize patient safety. From a regulatory perspective, changes in higher order structure due to are particular interest but can be difficult monitor directly at elevated concentrations. The present report focuses on non-native antistreptavidin (AS) IgG1 30 mg/mL under solution conditions that prior work dilute concentrations (e.g., 1 mg/mL) indicated would result...
Methionine oxidation is a major degradation pathway in therapeutic proteins which can impact the structure and function of as well risk to drug product quality. Detecting Met by peptide mapping followed liquid chromatography with mass spectrometry (LC–MS) industry standard but also labor intensive susceptible artifacts. In this work, vibrational difference spectroscopy combination 18O isotopic shift enabled us demonstrate application Raman FTIR techniques for detection quantification various...
Inverse Kirkwood–Buff (KB) solution theory can be used to relate macroscopic quantities with molecular scale interactions and correlation functions, in the form of KB integrals. Protein partial specific volumes (V̂2) from high-precision density measurements quantify solvent–solute solute–solute Currently, general expressions for V̂2 as a function cosolute concentration (c3) have been provided only binary ternary solutions. We derive multicomponent expression terms relevant integrals case low...