A5037802128- Pesticide Residue Analysis and Safety
- Analytical Chemistry and Chromatography
- Antibiotics Pharmacokinetics and Efficacy
- Metabolomics and Mass Spectrometry Studies
- Analytical chemistry methods development
- Analytical Methods in Pharmaceuticals
- Mass Spectrometry Techniques and Applications
- Traditional Chinese Medicine Analysis
- Phytochemicals and Antioxidant Activities
- Tea Polyphenols and Effects
Canadian Food Inspection Agency
2014-2025
This paper presents an application of ultrahigh-performance liquid chromatography and electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap) for determination 166 pesticide residues in fruits vegetables. Pesticides were extracted from the samples using QuEChERS (quick, easy, cheap, effective, rugged, safe) procedure. UHPLC/ESI Q-Orbitrap MS (i.e., full scan) acquired data quantification, dd-MS(2) data-dependent obtained product-ion spectra...
This paper presents an application of ultrahigh-performance liquid chromatography electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap MS) for the determination 451 pesticide residues in fruits and vegetables. Pesticides were extracted from samples using QuEChERS (quick, easy, cheap, effective, rugged, safe) procedure. UHPLC/ESI MS full scan mode acquired data quantification, Full MS/dd-MS(2) (i.e., data-dependent mode) obtained product ion...
A semiautomated qualitative method for target screening of 448 pesticide residues in fruits and vegetables was developed validated using ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap). The Q-Orbitrap Full MS/dd-MS2 (data dependent acquisition) used to acquire product-ion spectra individual pesticides build a compound database or an MS library, while its MS/DIA independent utilized...
This paper presents the development and validation of a multiclass method for analysis veterinary drug residues in milk using ultrahigh performance liquid chromatography electrospray ionization quadrupole Orbitrap mass spectrometry (UHPLC/ESI Q-Orbitrap). The 12 classes drugs (a total 125) included this study were endectocides, fluoroquinolones, ionophores, macrolides, nitroimidazole, NSAIDs, β-lactams, penicillins, phenicols, sulfonamides, tetracyclines, aminoglycosides. Veterinary...
Abstract Background Cost-effective multi-residue pesticide methods with a broad detection scope are desired for risk-based monitoring programs. Objective The aim was to evaluate the nDATA (non-target Data Acquisition Target Analysis) workflow using UHPLC/ESI quadrupole-Orbitrap (Q-Orbitrap) mass spectrometry and semi-quantitate 1094 pesticides in fruits vegetables. Methods Pesticide extracts from fresh produce were prepared QuEChERS procedure. carried out by utilizing Q-Orbitrap Full MS scan...
A nontarget Data Acquisition for Target Analysis (nDATA) workflow was developed to screen pesticides in fresh produce based on ultrahigh-performance liquid chromatography-high-resolution full scan mass spectrometry/variable data-independent tandem spectrometry acquisition (LC-FS MS/vDIA MSMS) and a pesticide database. The MSMS spectral library generated create database consisting of 1087 authentic standards. retention time (±0.5 min), precursor ion (≤± 5 ppm), product ions ppm) were...
Applications of liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) and ultrahigh-pressure quadrupole time-of-flight (UHPLC QqTOF MS) for the determination 148 pesticides in berry fruits are presented this study. Pesticides were extracted from berries using a procedure known as QuEChERS (quick, easy, cheap, effective, rugged, safe). Quantification, with an analytical range 5 to 500 μg/kg, was achieved matrix-matched standard calibration curves isotopically...
Nontarget data acquisition for target analysis (nDATA) workflows using liquid chromatography-high-resolution accurate mass (LC-HRAM) spectrometry, spectral screening software, and a compound database have generated interest because of their potential pesticides in foods. However, these procedures particularly the instrument processing software need to be thoroughly evaluated before implementation routine analysis. In this work, 25 laboratories participated collaborative study evaluate an...
This paper presents the applications of LC-electrospray ionization (ESI)/MS/MS and ultra-HPLC (UHPLC)/ESI quadrupole (Qq)-time-of-flight (TOF) MS for determination 141 pesticides in tea. Pesticides were extracted cleaned up from tea with a modified quick, easy, cheap, effective, rugged, safe method using graphitized carbon black primary-secondary amine sorbents. Quantification was achieved matrix-matched standard calibration curves isotopically labeled standards or chemical analog as...
A comparison of ultrahigh performance liquid chromatography (UHPLC) with a 2.6 μm core–shell particle column (Kinetex C18) and conventional (LC) 3 porous (Atlantis dC18), coupled electrospray ionization tandem mass spectrometry (ESI-MS/MS), for the determination 151 pesticides in grains is presented this study. Pesticides were extracted from grain samples using procedure known as QuEChERS (quick, easy, cheap, effective, rugged, safe). Quantification, an analytical range 5 to 500 μg/kg, was...
This paper presents the application of ultra-high performance LC (UHPLC) and MS for determination 151 pesticides in soybeans pulses. A core-shell particle (2.6 micro m size) column a fully porous sub-2 microm (1.7 showed comparable chromatographic resolution separation, increasing selectivity, reducing analysis time. UHPLC was coupled with either triple quadrupole mass analyzer (MS/MS) or Orbitrap (namely Orbital trap) spectrometer (Q-Orbitrap MS), which possesses fast data acquisition...
Abstract Background Effective and expansive methods for multiresidue pesticide analysis are desired routine monitoring programs. These complex, especially when several hundred pesticides involved. Objective Two approaches to sort data identify isomers isobaric ions in mixtures were evaluated determine whether they could be differentiated by mass resolving power and/or chromatographic resolution. Method This study presents an application of ultra-high performance liquid chromatography...
This study explored the use of UHPLC/ESI Q-Orbitrap HRMS-based nontarget metabolomics and QuEChERS for fingerprinting profiling orange juices purposes assessing authenticity consumer protection. Full MS MS/dd-MS2 (TopN) data allowed principle component analysis (PCA), clustering, differential analysis, identification molecular markers. By made "From Concentrate" (FCs) "Not From (NFCs) were distinguished in PCA score plot. Common citrus flavonoids such as tangeretin, nobiletin, hesperidin,...
High sample throughput and effective multiresidue methods for screening, quantitation, identification are desired the analysis of a large number pesticides in routine monitoring programs food safety. This study was designed to explore use an UHPLC/ESI Q-Orbitrap nontarget data acquisition target (nDATA) workflow screening 655 quantifying small group 46 most likely incurred pesticide residues fruits vegetables single analysis. High-resolution mass spectrometers such as offer unique...
Triple quadrupole (MS/MS) and high-resolution mass spectrometry (HRMS), coupled with ultra-high performance (UHP) liquid chromatography (LC) or gas (GC), are technologies used to analyze pesticide residues in fruits vegetables. LC-MS/MS has been the gold standard for analysis of pesticides, offering reliable sensitivity, while LC-HRMS is expanding application serve as another benchmark. Method development testing scope expansion constantly required new generation spectrometers.This article...
This study explored the use of ultrahigh-performance liquid chromatography electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap MS) and three extraction methods that included Dilution (1:1 with solvent buffer), QuEChERS Step I, I&II for fingerprinting identification molecular authenticity markers in apple grape juices. Of methods, allowed most number compounds to be detected revealed differences between samples. However, yielded similar...