A5003620240- FOXO transcription factor regulation
- Hippo pathway signaling and YAP/TAZ
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
- Environmental Justice and Health Disparities
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Genomics and Chromatin Dynamics
- Fecal contamination and water quality
- Arsenic contamination and mitigation
- Immunotherapy and Immune Responses
- Autoimmune Bullous Skin Diseases
- Antifungal resistance and susceptibility
- Cutaneous lymphoproliferative disorders research
- Systemic Sclerosis and Related Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Fungal Infections and Studies
- Mycotoxins in Agriculture and Food
Mount Desert Island Biological Laboratory
2015-2024
Falmouth Hospital
2021
Dartmouth College
2017-2020
ABSTRACT Heterogeneity among Aspergillus fumigatus isolates results in unique virulence potential and inflammatory responses. How these drive specific immune responses how this affects fungally induced lung damage disease outcome are unresolved. We demonstrate that the highly virulent CEA10 strain is able to rapidly germinate within immunocompetent environment, inducing greater damage, vascular leakage, interleukin 1α (IL-1α) release than low-virulence Af293 strain, which germinates with a...
Exposure to arsenic (As) in well water is a well-documented public health issue for Maine and New Hampshire, as other states the United States abroad. Arsenic contamination of these locations primarily attributed metasedimentary bedrock that leaches As into groundwater. However, can also enter groundwater reserves from soils contaminated by historical use arsenical pesticides. Approximately half households Hampshire rely on private wells, many which have elevated As. exposure has been...
Doublets are usually considered an unwanted artifact of single-cell RNA-sequencing (scRNA-seq) and only identified in datasets for the sake removal. However, if cells have a juxtacrine interaction with one another
Abstract In Caenorhabditis elegans, germline expression programs are actively repressed in somatic tissue by components of the synMuv (synthetic multi-vulva) B chromatin remodeling complex, which include homologs tumor suppressors Retinoblastoma (Rb/LIN-35) and Malignant Brain Tumor (MBT/LIN-61). However, full scope pathways that suppress soma is unknown. To address this, we performed a mutagenesis screened for GFP-tagged PGL-1, core P-granule nucleating protein. Eight alleles were isolated...
Abstract Foxo1 is an essential transcription factor required for the survival and differentiation of memory CD8 T cells, yet it unclear whether these Foxo1-dependent functions are inherently coupled. To address this question, we examined effects different posttranslational modifications. Phosphorylation by Akt kinases at three distinct residues well characterized to inhibit transcriptional activity. However, effect phosphorylation within its DNA-binding domain serine 209 Mst1 kinase not...
Abstract Motivation Doublets are usually considered an unwanted artifact of single-cell RNA-sequencing (scRNA-seq) and only identified in datasets for the sake removal. However, if cells have a juxtacrine attachment to one another situ maintain this association through scRNA-seq processing pipeline that partially dissociates tissue, these doublets can provide meaningful biological information regarding interactions cell processes occurring analyzed tissue. This is especially true cases such...
Abstract Foxo1 is an essential transcription factor required for the differentiation and survival of memory CD8 T cells. activity heavily regulated by post translational modifications, yet it unclear whether cell in same manner as survival. Two S/T kinases, Akt1 Mst1 phosphorylate Foxo1. While mediated phosphorylation known to inhibit transcriptional activity, effect remains poorly understood. To determine impact regulation on Foxo1-dependent survival, we generated variants that mimic...
Abstract Precise tug-of-war between immunosuppressive regulatory T (Treg) cells and inflammatory IL-17-producing (Th17) ensures appropriate reactivity to pathogenic challenge. While an imbalance towards Tregs leads suboptimal responses as observed in cancer, unchecked Th17 activity autoimmunity. Though numerous mechanisms have been identified for how suppress effector cells, productive cell are believed result from passive escape of Treg suppression. Here we report a novel active role...
Abstract A precise tug-of-war between regulatory T (Treg) cells and IL-17-producing (Th17) CD4 ensures appropriate reactivity to pathogenic challenge. While an imbalance favoring Treg activity leads suboptimal responses, overactive Th17 promotes autoimmunity. Both represent a significant proportion of the tumor infiltrating lymphocytes (TILs) in melanoma patients. In this disease context, Tregs suppress effector via numerous mechanisms. Productive immune responses are believed result from...