A5065872475- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Dermatology and Skin Diseases
- Infectious Diseases and Mycology
- Nail Diseases and Treatments
- African Botany and Ecology Studies
- Plant Pathogens and Fungal Diseases
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Immune cells in cancer
- Ginger and Zingiberaceae research
- Antimicrobial Peptides and Activities
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Synthesis and Biological Evaluation
- Mycotoxins in Agriculture and Food
- Bone and Joint Diseases
- Cytokine Signaling Pathways and Interactions
- Advancements in Transdermal Drug Delivery
- Ginkgo biloba and Cashew Applications
- Plant Pathogens and Resistance
- Food Allergy and Anaphylaxis Research
- Polydiacetylene-based materials and applications
Chulalongkorn University
2018-2024
King Chulalongkorn Memorial Hospital
2021-2024
Yamagata Prefectural Central Hospital
2024
Dartmouth College
2014-2019
Dartmouth Hospital
2015
Of the over 250 Aspergillus species, fumigatus accounts for up to 80% of invasive human infections. A. produces galactosaminogalactan (GAG), an exopolysaccharide composed galactose and N-acetyl-galactosamine (GalNAc) that mediates adherence is required full virulence. Less pathogenic species were found produce GAG with a lower GalNAc content than expressed minimal amounts cell wall-bound GAG. Increasing minimally nidulans, either through overexpression nidulans epimerase UgeB or by...
Aspergillus fumigatus is a mold that causes severe pulmonary infections. Our knowledge of how A. growth controlled in the respiratory tract developing, but still limited. Alveolar macrophages, lung resident and airway epithelial cells constitute first lines defense against inhaled conidia. Subsequently, neutrophils inflammatory CCR2+ monocytes are recruited to prevent fungal growth. However, mechanism neutrophil macrophage recruitment after exposure remains an area ongoing investigation....
Aspergillus fumigatus is responsible for a disproportionate number of invasive mycosis cases relative to other common filamentous fungi. While many fungal factors critical infection establishment are known, genes essential disease persistence and progression ill defined. We propose that promote navigation the rapidly changing nutrient structural landscape characteristic represent untapped clinically relevant therapeutic targets. To this end, we find A. requires carbon catabolite repression...
ABSTRACT Heterogeneity among Aspergillus fumigatus isolates results in unique virulence potential and inflammatory responses. How these drive specific immune responses how this affects fungally induced lung damage disease outcome are unresolved. We demonstrate that the highly virulent CEA10 strain is able to rapidly germinate within immunocompetent environment, inducing greater damage, vascular leakage, interleukin 1α (IL-1α) release than low-virulence Af293 strain, which germinates with a...
Aspergillus fumigatus is an opportunistic human pathogen causing allergic reactions or systemic infections such as invasive pulmonary aspergillosis, especially in immunocompromised patients. The fungal cell wall the main component responsible for recognition by immune system, due to specific composition of polysaccharide carbohydrates exposed on surface called pathogen-associated molecular patterns (PAMPs). Key enzymes biosynthesis are a good target drug development. This report elucidates...
ABSTRACT Background: Nosocomial aspergillosis in patients with sepsis has emerged the past few years. Blockade of PD-1/PD-L pathway tended to become a promising therapeutic strategy as it improved outcome bacterial and postsepsis secondary fungal infection. Recently, controversial effects PD-1 blockade on infectious diseases, including aspergillosis, have been demonstrated; therefore, efficacy anti-PD-1 drug still remains be elucidated. Methods: Cecal ligation puncture (CLP) was conducted...
Given the rise of multidrug-resistant (MDR) Pseudomonas aeruginosa infections, alternative treatments are needed. Anti-pseudomonal phage therapy shows promise, but its clinical application is limited due to development resistance and a lack biofilm penetration. Recently, adjuvants like CaEDTA have shown ability enhance effectiveness combined antimicrobial agents. Here, we tested phage-adjuvant combination demonstrated intranasally inhaled (KKP10) + in addition ceftazidime/avibactam (CZA) for...
Trehalose biosynthesis is found in fungi but not humans. Proteins involved trehalose are essential for fungal pathogen virulence humans and plants through multiple mechanisms. Loss of canonical genes the human Aspergillus fumigatus significantly alters cell wall structure integrity, though mechanistic link between these virulence-associated pathways remains enigmatic. Here we characterize genes, called tslA tslB, which encode proteins that contain domains similar to those corresponding...
Alkyl quinolone has been proven to be a privileged scaffold in the antimicrobial drug discovery pipeline. In this study, series of new 4-hydroxy-2-quinolinone analogs containing long alkyl side chain at C-3 and broad range substituents on C-6 C-7 positions were synthesized. The antibacterial antifungal activities these against Staphylococcus aureus, Escherichia coli, Aspergillus flavus investigated. structure-activity relationship study revealed that length chain, as well type substituent,...
SREBP transcription factors play a critical role in fungal virulence; however, the mechanisms of sterol regulatory element binding protein (SREBP) activation pathogenic fungi remains ill-defined. Screening Neurospora crassa whole-genome deletion collection for genes involved hypoxia responses identified gene an uncharacterized rhomboid protease homolog, rbdB, required growth under hypoxic conditions. Loss rbdB Aspergillus fumigatus also inhibited In addition, A. ΔrbdB strain displayed...
Regulation of fungal cell wall biosynthesis is critical to maintain integrity in dynamic infection microenvironments. Genes involved this response that impact fitness and host immune responses remain be fully defined. In study, we observed a yeast
GPI-anchoring is a universal and critical post-translational protein modification in eukaryotes. In fungi, many cell wall proteins are GPI-anchored, disruption of GPI-anchored impairs integrity. After being synthesized attached to target proteins, GPI anchors undergo on lipid moieties. spite its importance for functions, our current knowledge remodelling pathogenic fungi limited. this study, we characterized the role putative protein, designated PerA, human fungus Aspergillus fumigatus. PerA...
Background: Secondary fungal infection is a major complication in patients with sepsis-associated immunosuppression.However, sepsis-induced immune alterations related to susceptibility have not been well characterized.Objective: To determine kinetic changes the phenotype by determining proportion of T cells, B cells and macrophages, especially expression an exhaustion marker PD-1, murine sepsis.In addition, these were assessed relation secondary infection.Methods: Cecal ligation puncture...
Aspergillus flavus is one of the most common isolates from patients with fungal infections. infection usually treated antifungal agents, but side effects these agents are common. Trehalase an essential enzyme involved in metabolism, and trehalase inhibitor, validamycin A, has been used to prevent infections agricultural products. In this study, we observed that A significantly increased trehalose levels A. conidia delayed germination, including decreased adherence. addition, amphotericin B...
Background: Increased rates of Staphylococcus aureus resistance and its morbidity mortality have raised concern about the strategy antibiotic use.
Human Sporotrichosis is an infection caused by dimorphic fungus, Sporothrix schenckii complex, via direct inoculation. We are herein report proven 2 cases of sporotrichosis along with a literature review about human in the southeast Asian region. The first case was 76-year-old female non-progressive erythematous plaque at right ankle. second 36-year-old sporotrichoid lesion for six weeks. Both were treated itraconazole excellent outcome.
Abstract Background Pythium , soil-borne plant pathogens, are in the class Oomycetes. They not true fungi, but related to diatom and algae. There two human pathogens including P. insidiosum aphanidermatum . To date, only one case of pythiosis caused by has been reported. We present herein first vascular Asia. Case presentation A 47-year-old Thai woman, living North Thailand, with ß thalassemia/hemoglobin E presented acute recurrent arterial insufficiency both legs. Emergent embolectomy clot...
T-cell prolymphocytic leukemia (T-PLL) is a rare, mature which usually presents with aggressive behavior. We report an asymptomatic T-PLL patient diagnosed by clinical features, lymphocyte morphology, and flow cytometry. Incidentally, she was found to have lymphocytosis lymphadenopathy. Flow cytometry from blood revealed abnormally increased CD4+ population. receptor clonality assessment next-generation sequencing dominant clone in the ß-chain constant region. No pathogenic mutations 25...
The increasing occurrence of hospital-associated infections, particularly bacteremia, caused by extensively drug-resistant (XDR) carbapenemase-producing colistin-resistant Klebsiella pneumoniae highlights a critical requirement to discover new therapeutic alternatives. Bacteriophages having host-specific bacteriolytic effects are promising alternatives for combating these pathogens. Among 12 phages isolated from public wastewater in Thailand, two phages-vB_kpnM_05 (myovirus) and vB_kpnP_08...
Candida glabrata is a common non-albicans species found in patients with candidiasis and it sometimes develops antifungal resistance. Human beta-defensin-3 (hBD-3) an antimicrobial peptide of immune system active against various types microbes including spp. This study investigated activity hBD-3 its synergistic effect first-line agent on C. clinical isolates. were characterised candidiasis. The activities fluconazole evaluated using Broth microdilution assay. these two agents was determined...
Methylene blue-mediated photodynamic therapy as an antimicrobial has been reported to improve onychomycosis.To compare the short-term efficacy of methylene (MB-PDT) and 5% amorolfine nail lacquer (AMO) for toenail onychomycosis using higher intensity shorter total treatment period than previously reported.Twenty-seven toenails with were randomized receive either six biweekly sessions MB-PDT or AMO twelve weeks. Dermoscopic photography was used severity index assessment under a dermoscopic...
Globba schomburgkii Hook.f. is an ornamental plant that has recently found increasing demand as cut flowers, hence generating a significant number of by-products from different parts the plant. To investigate further applications these by-products, twelve crude extracts rhizomes, stalks, leaves, and flowers were prepared by serial exhaustive extraction. The volatile composition was analyzed GC/MS; total 89 compounds identified, most which sesquiterpenes well some labdane-type diterpenes....