A5025986701- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Research on Leishmaniasis Studies
- Protein Tyrosine Phosphatases
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Amoebic Infections and Treatments
- Blood disorders and treatments
- Parasites and Host Interactions
- Macrophage Migration Inhibitory Factor
- Bacterial Infections and Vaccines
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Autoimmune and Inflammatory Disorders Research
- Infections and bacterial resistance
- Trypanosoma species research and implications
- Ocular Infections and Treatments
- Asthma and respiratory diseases
- Plant Pathogens and Fungal Diseases
- Actinomycetales infections and treatment
- Pediatric health and respiratory diseases
- Bioactive Compounds and Antitumor Agents
- Polysaccharides and Plant Cell Walls
- Infectious Diseases and Mycology
McGill University
2010-2021
McGill University Health Centre
2011-2021
Institute of Infection and Immunity
2020
Of the over 250 Aspergillus species, fumigatus accounts for up to 80% of invasive human infections. A. produces galactosaminogalactan (GAG), an exopolysaccharide composed galactose and N-acetyl-galactosamine (GalNAc) that mediates adherence is required full virulence. Less pathogenic species were found produce GAG with a lower GalNAc content than expressed minimal amounts cell wall-bound GAG. Increasing minimally nidulans, either through overexpression nidulans epimerase UgeB or by...
The mold Aspergillus fumigatus causes invasive infection in immunocompromised patients. Recently, galactosaminogalactan (GAG), an exopolysaccharide composed of galactose and N-acetylgalactosamine (GalNAc), was identified as a virulence factor required for biofilm formation. molecular mechanisms underlying GAG biosynthesis GAG-mediated formation were unknown. We cluster five coregulated genes that dysregulated GAG-deficient mutants whose gene products share functional similarity with proteins...
Leishmania parasites are able to secure their survival and propagation within host by altering signalling pathways involved in the ability of macrophages kill pathogens or engage adaptive immune system. An important step this evasion process is activation protein tyrosine phosphatase SHP-1 Leishmania. has been shown directly inactivate JAK2 Erk1/2 play a role negative regulation several transcription factors macrophage activation. These alterations contribute inactivation critical functions...
Aspergillus fumigatus is an opportunistic mold that infects patients who are immunocompromised or have chronic lung disease, causing significant morbidity and mortality in these populations. While the factors governing host response to A. remain poorly defined, neutrophil recruitment site of infection critical clear fungus. Galectin-3 a mammalian β-galactose-binding lectin with both antimicrobial immunomodulatory activities, however role galectin-3 defense against molds has not been studied....
Leishmania parasites have the ability to modify macrophage signaling pathways in order survive and multiply within its mammalian host. They are also known invade other cells including neutrophils, fibroblasts dendritic (DCs). DCs an important role immunity as link between innate adaptive immunity, necessary for development of effective response; however, impact mexicana infection on has been poorly studied. Herein, we report that rapidly induced DC protein tyrosine phosphatases activity,...
Airway colonization by the mold Aspergillus fumigatus is common in patients with underlying lung disease and associated chronic airway inflammation. Studies probing inflammatory response to A. hyphae have been hampered lack of a model immunocompetent mice. By infecting mice intratracheally conidia embedded agar beads (Af beads), we established an vivo study natural history live hyphae. Histopathological examination galactomannan assay homogenates demonstrated that exited persisted lungs up...
Impaired delivery of antifungals to hyphae within necrotic lesions is thought contribute therapeutic failure in invasive pulmonary aspergillosis (IPA). We hypothesized that transfusion leukocytes loaded ex vivo with the lipophilic antifungal posaconazole could improve sites established infection and outcome experimental IPA. The HL-60 leukemia cell line was differentiated a neutrophil-like phenotype (differentiated [dHL-60] cells) then exposed range concentrations. functional capacity...
Interleukin-1 alpha (IL-1α) and interleukin-1 beta (IL-1β) are pro-inflammatory cytokines that induced after Cryptococcus neoformans infection activate the receptor type I (IL-1RI). To establish role of IL-1RI signaling in protection against cryptococcal infection, we analyzed wild-type (WT) IL-1RI-deficient (IL-1RI-/-) mice on BALB/c background. IL-1RI-/- had significantly reduced survival compared to WT intratracheal challenge with C. 52D. Microbiological analysis showed a significant...
Aspergillus fumigatus airway infections are associated with increased rates of hospitalizations and declining lung function in patients chronic disease. While the pathogenesis invasive A. is well studied, little known about development progression infections. Previous studies have demonstrated a critical role for IL-1 cytokines, IL-1α IL-1β enhancing pulmonary neutrophil recruitment during aspergillosis. Here we use mouse model infection to study these cytokines immunocompetent mice. In...
A 56-year-old Asian woman presented with an upper eyelid mass. The lesion was exposed after eversion of the revealing a thickened tarsus yellowish areas. Working diagnosis sebaceous carcinoma. Biopsy performed. Histopathological studies showed mycotic eumycetoma Splendore-Hoeppli phenomena and - microbiologic cultures grew Scedosporium apiospermum. patient started on voriconazole 200 mg po bid adequate serum levels. complete response observed 18 weeks therapy. To best our knowledge, this is...