A5003748282- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Insect and Arachnid Ecology and Behavior
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Plant and animal studies
- Mycotoxins in Agriculture and Food
- Chemical Synthesis and Analysis
- X-ray Diffraction in Crystallography
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Crystallization and Solubility Studies
- Sesquiterpenes and Asteraceae Studies
- Synthesis and Catalytic Reactions
- Asymmetric Hydrogenation and Catalysis
- Enzyme Structure and Function
- Insect symbiosis and bacterial influences
- Click Chemistry and Applications
- Pharmacological Effects of Natural Compounds
- Plant Toxicity and Pharmacological Properties
- Insect and Pesticide Research
- Neurobiology and Insect Physiology Research
- Fluorine in Organic Chemistry
- Polyamine Metabolism and Applications
- Genomics and Phylogenetic Studies
Scripps Institution of Oceanography
2022-2024
University of California, San Diego
2022-2024
University of Bonn
2015-2023
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2019
Species of the Fusarium fujikuroi species complex (FFC) cause a wide spectrum often devastating diseases on diverse agricultural crops, including coffee, fig, mango, maize, rice, and sugarcane. Although within FFC are difficult to distinguish by morphology, their genes share 90% sequence similarity, they can differ in host plant specificity life style. also produce structurally secondary metabolites (SMs), mycotoxins fumonisins, fusarins, fusaric acid, beauvericin, phytohormones...
Filamentous fungi produce a vast array of secondary metabolites (SMs) and some play role in agriculture or pharmacology. Sequencing the rice pathogen Fusarium fujikuroi revealed presence far more SM-encoding genes than known products. SM production is energy-consuming thus tightly regulated, leaving majority gene clusters silent under laboratory conditions. One important regulatory layer biosynthesis involves histone modifications that render underlying either poised for transcription. Here,...
Sea sponges are the largest marine source of small-molecule natural products described to date. Sponge-derived molecules, such as chemotherapeutic eribulin, calcium-channel blocker manoalide, and antimalarial compound kalihinol A, renowned for their impressive medicinal, chemical, biological properties. Sponges contain microbiomes that control production many isolated from these invertebrates. In fact, all genomic studies date investigating metabolic origins sponge-derived small molecules...
Abstract Two sesquiterpene cyclases from Fusarium fujikuroi were expressed in Escherichia coli and purified. The first enzyme was inactive because of a critical mutation, but activity restored by sequence correction through site‐directed mutagenesis. mutated two naturally functional homologues other fusaria converted farnesyl diphosphate into guaia‐6,10(14)‐diene. second produced eremophilene. absolute configuration guaia‐6,10(14)‐diene elucidated enantioselective synthesis, while that...
Fusaric acid (FSA) is a mycotoxin produced by several fusaria, including the rice pathogen Fusarium fujikuroi. Genes involved in FSA biosynthesis were previously identified as cluster containing polyketide synthase (PKS)-encoding (FUB1) and four additional genes (FUB2-FUB5). However, biosynthetic steps leading to well origin of nitrogen atom, which incorporated into backbone, remained unknown. In this study, seven (FUB6-FUB12) via manipulation global regulator FfSge1. The extended FUB gene...
Macrotermitinae termites have farmed fungi in the genus Termitomyces as a food source for millions of years. However, biochemical mechanisms orchestrating this mutualistic relationship are largely unknown. To deduce fungal signals and ecological patterns that relate to stability symbiosis, we explored volatile organic compound (VOC) repertoire from Macrotermes natalensis colonies. Results show mushrooms emit VOC pattern differs mycelium grown gardens laboratory cultures. The abundance...
Abstract Highlights von November 2023 bis 2024: die erste Einelektron‐C–C‐σ‐Bindung und Anti‐Bredt‐Verbindung; gesättigte Heterocyclen elektrochemisch funktionalisieren; Ausrichten diskotischer Flüssigkristalle; enantioselektive Wagner‐Meerwein‐Umlagerung reiner Aliphaten; photokatalytisch Furanen zu Pyrrolen; mit Ammoniak primären Arylaminen; Metallschrott recyceln ionischen Flüssigkeiten; terminale Alkene Ni‐Katalysatoren zum (Z)‐ oder (E)‐Alken isomerisieren;neue Fungizide, Medikamente Alkaloide.
GATA-type transcription factors (TFs) such as the nitrogen regulators AreA and AreB, or light-responsive TFs WC-1 WC-2, play global roles in fungal growth development. The conserved GATA TF NsdD is known an activator of sexual development key repressor conidiation Aspergillus nidulans, light-regulated macroconidia formation Botrytis cinerea. In present study, we functionally characterized ortholog Fusarium fujikuroi, named Csm1. Deletion this gene resulted elevated microconidia wild-type...
Three terpene synthases from the termite associated fungus Termitomyces were functionally characterized as (+)-intermedeol synthase, (-)-γ-cadinene synthase and (+)-germacrene D-4-ol with germacrene first reported enzyme that produces (+)-enantiomer. The enzymatic mechanisms thoroughly investigated by incubation isotopically labeled precursors to follow stereochemical courses of single reaction steps in catalysis. role putative active site residues was tested directed mutagenesis a highly...
Macrotermitine termites have domesticated fungi in the genus Termitomyces as their primary food source using predigested plant biomass. To access full nutritional value of lignin-enriched biomass, termite-fungus symbiosis requires depolymerization this complex phenolic polymer. While most previous work suggests that lignocellulose degradation is accomplished predominantly by fungal cultivar, our current understanding underlying biomolecular mechanisms remains rudimentary. Here, we provide...
Dimethylsulfoniopropionate (DMSP) catabolism of marine bacteria plays an important role in and global ecology. The genome Ruegeria pomeroyi DSS-3, a model organism from the Roseobacter group, harbours no less than three genes for different DMSP lyases (DddW, DddP DddQ) that catalyse degradation to dimethyl sulfide (DMS) acrylate. Despite their apparent similar function these enzymes show significant overall sequence identity. In this work DddQ DddW R. homolog Phaeobacter inhibens DSM 17395...
Abstract Zwei Sesquiterpencyclasen aus Fusarium fujikuroi wurden in Escherichia coli exprimiert und gereinigt. Das erste Enzym war wegen einer kritischen Mutation inaktiv, die Aktivität konnte aber durch Sequenzkorrektur per ortsgerichteter Mutagenese wiederhergestellt werden. mutierte zwei natürlicherweise funktionale Homologe anderen Fusarien konvertierten Farnesyldiphosphat zu Guaia‐6,10(14)‐dien. zweite produzierte Eremophilen. Die absolute Konfiguration von Guaia‐6,10(14)‐dien wurde...
Red algae or seaweeds produce highly distinctive halogenated terpenoid compounds, including the pentabromochlorinated monoterpene halomon that was once heralded as a promising anticancer agent. The first dedicated step in biosynthesis of these natural product molecules is expected to be catalyzed by terpene synthase (TS) enzymes. Recent work has demonstrated an emerging class type I TSs red algal biosynthesis. However, only one such enzyme from notoriously haloterpenoid-producing alga...
Dimethylallyltryptophan synthases catalyze the regiospecific transfer of (oligo)prenylpyrophosphates to aromatic substrates like tryptophan derivatives. These reactions are key steps in many biosynthetic pathways fungal and bacterial secondary metabolites. In vitro investigations on recombinant DMATS1Ff from Fusarium fujikuroi identified enzyme as first selective reverse tryptophan-N-1 prenyltransferase origin. The was also able N-geranylation tryptophan. shown be phylogenetically related...
2‐Pyrones are frequently produced by microorganisms and often exhibit interesting bioactivities. Therefore, a short easy synthetic access to these natural products is desirable. Synthetic routes nectriapyrone, gibepyrone A, racemic gulypyrone (+)‐germicidin C, ( ent )‐desoxygermicidin C )‐prolipyrone A via modular approach presented, allowing the assignment of absolute configurations latter three chiral compounds. The method failed for synthesis )‐phomapyrone B that was thus synthesized...
Cultures of a termite-associated and free-living member the fungal genus Podaxis, revived from spores maintained in century-old herbarium collections, were analyzed for their insecticidal antimicrobial effects. Their secondary metabolomes explored to uncover possible adaptive mechanisms termite association, dereplication LC-HRMS/MS data sets led isolation podaxisterols A-D (1-4), modified ergosterol derivatives that result Diels-Alder reaction with endogenous nitrosyl cyanide. Chemical...
Abstract Unter anderem das hat die Organik im letzten Jahr bewegt: milde Oxidation mit Elektrochemie, zu enantiomerenreinen Sulfonylverbindungen, Flüssigkristallphasen erkennen maschinellem Lernen, CO 2 reagiert Succinat und Carbamaten, eine Alternative Bisphenol A, Subporphyrine, photoschaltbare Spinmaterialien, photochemische Thiophen‐Ringerweiterung, Peptide werden Bor versehen cyclisiert. Die Zusammenstellung des Trendberichts koordiniert Martin Breugst, Universität Chemnitz.
<title>Abstract</title> Octocorals are unique among metazoans in their prolific production of bioactive terpenoid natural products that rival the chemical diversity plants and microbes. We recently established these cnidarians uniformly express terpene cyclases encoding genes often reside within putative biosynthetic gene clusters (BGCs), a feature uncommon animal genomes. In this work, we report discovery characterization widespread cluster family for biosynthesis briarane diterpenoids...
Isochamigrene, a side product of the trichodiene synthase, which is key enzyme from biosynthesis trichothecene mycotoxins Fusarium spp., was enantioselectively synthesised and compared to natural sporotrichioides. As result, its absolute configuration assigned (S)-isochamigrene. Implications for recently extensively discussed cyclisation mechanism towards products are discussed.
Abstract Macrotermitine termites have domesticated fungi in the genus Termitomyces as their primary food source using pre-digested plant biomass. To access full nutritional value of lignin-enriched biomass, termite-fungus symbiosis requires depolymerization this complex phenolic polymer. While most previous work suggests that lignocellulose degradation is accomplished predominantly by fungal cultivar, our current understanding underlying biomolecular mechanisms remains rudimentary. Here, we...