- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Mycotoxins in Agriculture and Food
- Microbial metabolism and enzyme function
- Electromagnetic Fields and Biological Effects
- Tissue Engineering and Regenerative Medicine
- Advanced biosensing and bioanalysis techniques
- Telomeres, Telomerase, and Senescence
- Biofield Effects and Biophysics
- Microbial Metabolites in Food Biotechnology
- Muscle Physiology and Disorders
- Legume Nitrogen Fixing Symbiosis
- Salivary Gland Disorders and Functions
- DNA Repair Mechanisms
- CAR-T cell therapy research
- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Fluoride Effects and Removal
- RNA Interference and Gene Delivery
- Protein purification and stability
Suranaree University of Technology
2013-2025
Rajamangala University of Technology
2024-2025
Background/Objectives: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate differences in salivary microbiota metabolite profiles between individuals with halitosis periodontitis healthy controls. Additionally, expanded study examine how malodorous compounds interact human squamous carcinoma (HSC-4) cells. Methods: Saliva samples were collected analyzed using Ultra-High Performance...
Abstract The glutamine synthetase (GS)-based Chinese hamster ovary (CHO) selection system is an attractive approach to efficiently identify suitable clones in the cell line generation process for biologics manufacture, which GS-knockout (GS-KO) CHO lines are commonly used. Since genome analysis indicated that there two GS genes cells, deleting only 1 gene could potentially result activation of other genes, consequently reducing efficiency. Therefore, this study, both identified on chromosome...
A simple and reliable method for the detection of specific nitrogen-fixing bacteria in both free-living bacteroid forms is essential development application biofertilizer. Traditionally, a polyclonal antibody generated from an immunized rabbit was used detection. However, disadvantages using include limited supply cross-reactivity to related bacterial strains. This first report on phage display technology generation recombinant monoclonal monitoring form plant nodules. Bradyrhizobium sp....
The effects of water treated with an electromagnetic field (EMF) were investigated on two biological systems, humans and plants. Purified de-ionised was by (1) boiling, (2) exposure to microwave radiation, (3) low frequency oscillation molecular resonance effect technology (MRET), before being used prepare media for culturing human peripheral blood mononuclear cells (PBMC) from three healthy females. Our results indicated that PBMC culture in MRET-activated medium showed significantly less...
Ochratoxin A (OTA) is a mycotoxin commonly found in agricultural products and can accumulate the blood tissues after that consuming contaminated food. Recombinant single-chain antibody fragments (scFv) against OTA were selected from phage display libraries. After of one round biopanning BSA-conjugated (OTA-BSA), 52 6 clones displaying scFv antibodies isolated human (Yamo I.3) rabbit (Bozmix I.2) Two (one each libraries, i.e., yOTA1e3 bOTA2a9) showed binding to free toxin by competitive...
Abstract Efficient selection and production of antibody fragments in microbial systems remain to be a challenging process. To optimize single-chain variable (scFvs), we have chosen five model targets, 1) hapten, Zearalenone (ZEN) mycotoxin, along with infectious agents 2) rabies virus, 3) Propionibacterium acnes , 4) Pseudomonas aeruginosa cancer cell 5) acute myeloid leukemia line (HL-60). The scFv binders were affinity selected from non-immunized human phage display library genetically...