A5003707748- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Mycotoxins in Agriculture and Food
- Signaling Pathways in Disease
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
- CAR-T cell therapy research
- Synthesis and Biological Evaluation
- Food Safety and Hygiene
- Legume Nitrogen Fixing Symbiosis
- Genetically Modified Organisms Research
- Meat and Animal Product Quality
- Nanocomposite Films for Food Packaging
- Microbial metabolism and enzyme function
- Studies on Chitinases and Chitosanases
- Immunodeficiency and Autoimmune Disorders
- Fluoride Effects and Removal
- Proteins in Food Systems
- CRISPR and Genetic Engineering
- NF-κB Signaling Pathways
- Advanced Biosensing Techniques and Applications
- Chronic Lymphocytic Leukemia Research
Suranaree University of Technology
2009-2024
Abstract Background Phage display technology is a powerful new tool for making antibodies outside the immune system, thus avoiding use of experimental animals. In early days, it was postulated that this technique would eventually replace hybridoma and animal immunisations. However, since emerged more than 20 years ago, there have only been handful reports on construction application phage antibody libraries world-wide. Results Here we report simplest highly efficient method useful human...
Chito-oligosaccharides (CHOS) are oligomers of D-glucosamine and N -acetyl-glucosamine. Anti-inflammatory activities a wide variety CHOS mixtures have previously been reported, mainly based on studies with mouse models murine macrophages. Since the human immune systems quite different, gaining insight into activity cell lines, using well-characterized mixtures, is considerable interest. Bacillus subtilis chitosanase (BsCsn46A) can efficiently convert chitosan to water soluble low molecular...
Abstract The glutamine synthetase (GS)-based Chinese hamster ovary (CHO) selection system is an attractive approach to efficiently identify suitable clones in the cell line generation process for biologics manufacture, which GS-knockout (GS-KO) CHO lines are commonly used. Since genome analysis indicated that there two GS genes cells, deleting only 1 gene could potentially result activation of other genes, consequently reducing efficiency. Therefore, this study, both identified on chromosome...
A human antiaflatoxin B1 (AFB1) scFv antibody (yAFB1-c3), selected from a naı̈ve phage-displayed library, was used as template for improving and analysis of antibody–ligand interactions using the chain-shuffling technique. The variable-heavy variable-light (VH/VL)-shuffled library constructed VH 25 preselected clones recombined with VL yAFB1-c3 vice versa. Affinity selection these libraries demonstrated that domain played an important role in binding to free AFB1. Therefore, next step,...
The application of recombinant antibodies for the analysis foods and food contaminants is now a major focus, given their capacity to be engineered tailor specificity, enhance stability, modify structural formats fit desired analytical platform. In this study, human scFv antibody fragments generated against aflatoxin B1 (AFB1) were selected as model explore effect on binding activity evaluate potential use immunoreagents contaminant analysis. Four (AFB1), previously isolated by chain...
CD99 was demonstrated to be a potential target for antibody therapy on T-acute lymphoblastic leukemia (T-ALL). The ligation of by certain monoclonal antibodies (mAbs) induced T-ALL apoptosis. However, the molecular basis contributing apoptosis upon anti-CD99 mAb engagement remains elusive. In this study, using our generated clone MT99/3 (mAb MT99/3), strongly cell lines, but not in non-malignant peripheral blood cells. By transcriptome analysis, ligation, 13 apoptosis-related genes,...
The hydrolysis of milk proteins by the recombinant AprX‐SK37 protease and changes in rheological properties gel generated with A pr X ‐ SK 37 glucono‐δ‐lactone ( GDL ) were investigated. rennet selectively hydrolysed κ‐casein to yield a 16‐kDa band, while subtilisin all casein components. Milk treated only formed softer gel. Storage modulus (G′) values combined gels increased concentrations up 7 g/L. High tan δ was observed at 8.75 g/L alongside syneresis. is promising milk‐clotting enzyme...
Domain 1 of CD147 participates in matrix metalloproteinase (MMP) production and is a candidate for targeted therapy to prevent cancer invasion metastasis. A functional mouse anti-CD147 monoclonal antibody, M6-1B9, was found recognize domain CD147, its respective single-chain variable fragment (ScFvM61B9) subsequently generated. The EDLGS epitope M6-1B9 identified using the phage display peptide technique this study. For future clinical applications, humanized ScFv specific (HuScFvM61B9)...
In this study, we investigated the effect of adenosine and its derivative cordycepin on production yield a recombinant human monoclonal antibody (adalimumab) in two commonly used Chinese Hamster ovary (CHO) cell lines that have different gene amplification systems, namely CHO-DHFR
Human scFv antibody generated from phage display technology was successfully used for the generation of specific recombinant antibodies: yiN92-1e10 and yiDOA9-162 detection Bradyrhizobium strains SUTN9-2 DOA9, respectively.
Abstract Anti-interferon gamma autoantibodies (anti-IFN-γ autoAbs) neutralize the IFN-γ-mediated functions, contributing to immunodeficiency. A particular autoAb in patient serum had been previously demonstrated recognize same determinant on IFN-γ as neutralizing anti-IFN-γ monoclonal antibody clone B27 (B27 mAb). This study explored epitope recognized by mAb. The specific peptide sequence mAb, TDFLRMMLQEER, was retrieved from a phage display random library. Sequence alignment and homology...
Abstract Efficient selection and production of antibody fragments in microbial systems remain to be a challenging process. To optimize single-chain variable (scFvs), we have chosen five model targets, 1) hapten, Zearalenone (ZEN) mycotoxin, along with infectious agents 2) rabies virus, 3) Propionibacterium acnes , 4) Pseudomonas aeruginosa cancer cell 5) acute myeloid leukemia line (HL-60). The scFv binders were affinity selected from non-immunized human phage display library genetically...
Abstract Domain 1 of CD147 participates in matrix metalloproteinase (MMP) production and is a candidate for targeted therapy to prevent cancer invasion metastasis. A functional mouse anti-CD147 monoclonal antibody, M6-1B9, was found recognize domain CD147, its respective single-chain variable fragment (ScFvM61B9) subsequently generated. The EDLGS epitope M6-1B9 identified using the phage display peptide technique this study. For future clinical applications, humanized ScFv specific...