A5030870333- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Alzheimer's disease research and treatments
- Monoclonal and Polyclonal Antibodies Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Influenza Virus Research Studies
- Dementia and Cognitive Impairment Research
- Calcium Carbonate Crystallization and Inhibition
- Nutritional Studies and Diet
- Dermatological and Skeletal Disorders
- Infrared Thermography in Medicine
- Advanced Proteomics Techniques and Applications
- Cerebrovascular and genetic disorders
- Retinal Imaging and Analysis
- Neurological diseases and metabolism
- Genomics and Phylogenetic Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Nuclear Structure and Function
- Genetic Associations and Epidemiology
- Traditional Chinese Medicine Studies
- RNA Research and Splicing
- Galectins and Cancer Biology
- Mass Spectrometry Techniques and Applications
- Nanowire Synthesis and Applications
- Cancer Genomics and Diagnostics
Central South University
2022-2024
Third Xiangya Hospital
2022-2024
Xiangya Hospital Central South University
2022-2023
Xuzhou Medical College
2014-2022
Harvard University
1997-2017
Boston Children's Hospital
2012-2017
University of New Hampshire
2009-2016
Abstract Background Electroencephalogram (EEG) has emerged as a non-invasive tool to detect the aberrant neuronal activity related different stages of Alzheimer’s disease (AD). However, effectiveness EEG in precise diagnosis and assessment AD its preclinical stage, amnestic mild cognitive impairment (MCI), yet be fully elucidated. In this study, we aimed identify key biomarkers that are effective distinguishing patients at early stage monitoring progression AD. Methods A total 890...
Metastasis is the major cause of colorectal cancer (CRC) mortality. Emerging evidence suggests that long noncoding RNAs (lncRNAs) drive metastasis and their regulatory pathways could be targeted for preventing metastasis. However, underlying mechanisms lncRNAs in CRC remain poorly understood. Microarray analysis was used to screen differentially expressed lncRNAs. Transwell assays, fibronectin cell adhesion mouse models were utilized evaluate metastatic capacities vitro vivo. Chromatin...
Differences in ionization efficiency among neutral and sialylated glycans prevent direct quantitative comparison by their respective mass spectrometric signals. To overcome this challenge, we developed an integrated chemical strategy, Dual Reactions for Analytical Glycomics (DRAG), to quantitatively compare simultaneously MALDI-MS. Initially, two glycan samples be compared undergo reductive amination with 2-aminobenzoic acid 2-(13)[C6]-aminobenzoic acid, respectively. The different...
: CD39, a key ectonucleotidase that drives adenosine production, acts as critical immunosuppressive checkpoint in cancer. Although it has shown promise therapeutic target, clinical trials are demonstrating the need for more potent targeting approaches. This is driving innovation towards development of novel antibodies and exploration strategic combinations with range immunotherapies.
Procedures are detailed for a quantitative release of O-linked glycans from peptides that now provide shorter reaction time, possible identification sites, and quantification all products. The was initiated by mild base, dimethylamine, accelerated microwave radiation. Differential analysis using standard glycoproteins has shown improved efficiency concurrent with facile incorporation dimethylamine into the former sites. In situ glycan reduction insures protection against peeling is...
Current strategies to study N-glycoproteins in complex samples are often discrete, focusing on either N-glycans or N-glycosites enriched by sugar-based techniques. In this we report a simple and rapid sample preparation platform, the GlycoFilter, which allows comprehensive characterization of N-glycans, N-glycosites, proteins single workflow. Both PNGase F catalyzed de-N-glycosylation trypsin digestions accelerated microwave irradiation performed sequentially spin filter. peptides (including...
This report describes the structural details of a unique N-linked valence epitope on major protein within extrapallial (EP) fluid mollusk, Mytilus edulis. Fluids from this area are considered to be responsible for shell expansion by self-assembly process that provides an organic framework growth CaCO3 crystals. Previous reports our laboratories have described purification and amino acid sequence EP protein, which was found glycoprotein (EPG) approximately 28 KDa with 14.3% carbohydrate...
The pathogenic missense mutations of the gelsolin (GSN) gene lead to familial amyloidosis Finnish type (FAF); however, our previous study identified GSN frameshift existed in patients with Alzheimer's disease (AD). genotype-phenotype heterogeneity and role AD are unclear.In total, 1192 1403 controls were screened through whole genome sequencing, 884 enrolled for validation. Effects evaluated vitro. GSN, Aβ42, Aβ40 Aβ42/40 detected both plasma cerebrospinal fluid (CSF).Six P3fs K346fs (0.50%,...
Background Previous epidemiological studies have reported controversial results on the relationship between smoking and Alzheimer’s disease (AD). Therefore, we sought to assess association using Mendelian randomization (MR) analysis. Methods We used single nucleotide polymorphisms (SNPs) associated with quantity (cigarettes per day, CPD) from genome-wide (GWAS) of Japanese population as instrumental variables, then performed two-sample MR analysis investigate AD in a Chinese cohort (1,000...
The obesity rate among middle-aged and young adults in China is increasing annually, the incidence of cardiovascular diseases becoming more prevalent younger populations. However, it has not yet been reported whether associated with early vascular aging (EVA). This study aims to explore correlation between EVA adult health check-up populations, providing a reference for prevention diseases.
The relationships between multiple visual rating scales based on structural magnetic resonance imaging (sMRI) with disease severity and cerebrospinal fluid (CSF) biomarkers in patients Alzheimer’s (AD) were ambiguous. In this study, a total of 438 clinically diagnosed AD recruited. All participants underwent brain sMRI scan, medial temporal lobe atrophy (MTA), posterior (PA), global cerebral atrophy-frontal sub-scale (GCA-F), Fazekas scores visually evaluated. Meanwhile, was assessed by...
The 1918 H1N1 influenza virus was responsible for one of the most deadly pandemics in human history. Yet to date, structure component its virulence is still a mystery. In order search such component, neuraminidase (NA) antigen expressed, which led discovery an active form (tetramer) and inactive (dimer monomer) protein due different glycosylation. this report, N-glycans from both forms were released characterized by mass spectrometry. It found that glycans had 26% core-6 fucosylated, while...
We describe a novel solid-phase reversible sample-prep (SRS) platform that enables rapid sample preparation for concurrent proteome and N-glycome characterization nearly all protein samples. SRS utilizes uniquely functionalized, silica-based bead has strong affinity toward proteins with minimal to no peptides other small molecules. By leveraging this inherent size difference between peptides, permits high-capacity binding of proteins, removal molecules (detergents, metabolites, salts, etc.),...
Neisseria sicca 4320 expresses two carbohydrate-containing components with sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities that resemble those of lipooligosaccharide and lipopolysaccharide. Using matrix-assisted laser desorption ionization--time flight electrospray ionization mass spectrometry, we characterized a disaccharide carbohydrate repeating unit expressed by this strain. Gas chromatography identified the sugars composing as rhamnose N-acetyl-D-glucosamine....
Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a vaso-specific inflammatory marker that exacerbates atherosclerotic through responses. It can be used to predict the occurrence of adverse cardiovascular events and assess residual risk diseases. This study aims investigate correlation between smoking serum Lp-PLA2 levels in overweight obese men, provide evidence for preventing