A5035707136- Studies on Chitinases and Chitosanases
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Silk-based biomaterials and applications
- Genomics and Chromatin Dynamics
- Enzyme Catalysis and Immobilization
- Microbial Community Ecology and Physiology
- Phytochemical compounds biological activities
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Biochemical and Structural Characterization
University of California, San Diego
2022-2024
Princeton University
2016
Transcriptional repression is a pervasive feature of animal development. Here, we employ live-imaging methods to visualize the Snail repressor, which establishes boundary between presumptive mesoderm and neurogenic ectoderm early Drosophila embryos. target enhancers were attached an MS2 reporter gene, permitting detection nascent transcripts in living The transgenes exhibit initially broad patterns transcription but are refined by following mitosis. These observations reveal correlation...
A genome-scale metabolic model of V. splendidus 1A01 was reconstructed in this work. We offer solutions to the technical problems associated with reconstruction for a marine bacterial strain like 1A01, which arise largely from high salt concentration found both seawater and culture media that simulate seawater.
Resource competition strongly shapes microbial community dynamics and function. In polysaccharide-degrading communities, primary degraders secrete monomer-releasing enzymes exploiters consume released products without investing in enzymes. By investigating competitive strategies between chitin N-acetylglucosamine (GlcNAc) exploiters, we uncover several interacting factors, including GlcNAc liberation rates, diffusive loss, resource partitioning competitors, which determine viability growth...
Abstract While the Vibrio splendidus species is best known as an opportunistic pathogen in oysters, sp. 1A01 strain was first identified early colonizer of synthetic chitin particles incubated seawater. To gain a better understanding its metabolism, genome-scale metabolic model (GSMM) V. reconstructed. GSMMs enable us to simulate all reactions bacterial cell using Flux Balance Analysis. A draft built automated pipeline from BioCyc. Manual curation then performed based on experimental data,...
ABSTRACT Many biogeochemical functions involve bacteria utilizing solid substrates. However, little is known about the coordination of bacterial growth with kinetics attachment to and detachment from such In this quantitative study Vibrio sp. 1A01 growing on chitin particles, we reveal heterogeneous nature exponentially culture, comprised two co-existing subpopulations: a minority replicating particles non-replicating majority which was planktonic. This partition resulted high rate cell...