A5026590915- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Planarian Biology and Electrostimulation
- Cellular Mechanics and Interactions
- Pancreatic function and diabetes
- Congenital heart defects research
- Hippo pathway signaling and YAP/TAZ
- Gene Regulatory Network Analysis
- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Plant Molecular Biology Research
European Molecular Biology Laboratory
2024
European Molecular Biology Laboratory
2019
European Molecular Biology Laboratory
2019
University of California, Berkeley
2013-2016
Princeton University
2015-2016
Innovative Genomics Institute
2014
We present the use of recently developed live imaging methods to examine dynamic regulation even-skipped (eve) stripe 2 expression in precellular Drosophila embryo. Nascent transcripts were visualized via MS2 RNA stem loops. The eve transgene exhibits a highly pattern de novo transcription, beginning with broad domain during nuclear cycle 12 (nc12), and progressive refinement nc13 nc14. mature is surprisingly transient, constituting just ∼15 min ∼90-min period expression. Nonetheless, this...
Abstract The question of how metabolism impacts development is seeing a renaissance [1, 2]. How exerts instructive signaling functions one the central issues that need to be resolved. We tackled this in context mouse embryonic axis segmentation. Previous studies have shown changes carbon impact Wnt [3–6] and period segmentation clock [7], which controls timing Here, we reveal glycolysis tunes an anti-correlated manner: higher glycolytic flux slows down clock, vice versa. Transcriptome gene...
Report31 October 2019Open Access Transparent process Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation Ranjith Viswanathan Developmental Biology Unit, European Molecular Laboratory, Heidelberg, GermanyCollaboration for a joint PhD degree between EMBL and Heidelberg University, Faculty Biosciences Search more papers by this author Aleksandar Necakov Germany Department Biological Science, Brock St. Catharines, ON, Canada Mateusz Trylinski...
Transcriptional repression is a pervasive feature of animal development. Here, we employ live-imaging methods to visualize the Snail repressor, which establishes boundary between presumptive mesoderm and neurogenic ectoderm early Drosophila embryos. target enhancers were attached an MS2 reporter gene, permitting detection nascent transcripts in living The transgenes exhibit initially broad patterns transcription but are refined by following mitosis. These observations reveal correlation...
Abstract Spatio-temporal regulation of signalling pathways plays a key role in generating diverse responses during the development multicellular organisms. The signal dynamics transferring information vivo is incompletely understood. Here we employ genome engineering Drosophila melanogaster to generate functional optogenetic allele Notch ligand Delta (opto-Delta), which replaces both copies endogenous wild type locus. Using clonal analysis, show that activation blocks through cis-inhibition...