A5082570613- Non-Invasive Vital Sign Monitoring
- Protein Structure and Dynamics
- Fatty Acid Research and Health
- Folate and B Vitamins Research
- Cell Adhesion Molecules Research
- Blood Pressure and Hypertension Studies
- Hemodynamic Monitoring and Therapy
- Lipid Membrane Structure and Behavior
- Nuclear and radioactivity studies
- Immune Response and Inflammation
- Biochemical and Molecular Research
- Electric Vehicles and Infrastructure
- Eicosanoids and Hypertension Pharmacology
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Heart Rate Variability and Autonomic Control
- Epigenetics and DNA Methylation
- Developmental Biology and Gene Regulation
- Nuclear Materials and Properties
- Renal and related cancers
- Platelet Disorders and Treatments
- Advanced Battery Technologies Research
- Enzyme Structure and Function
- Nuclear reactor physics and engineering
First Affiliated Hospital of Xiamen University
2021-2024
Fujian Medical University
2021-2024
Southern University of Science and Technology
2024
Ji Hua Laboratory
2023
Shenzhen University
2023
Zhengzhou University
2020-2022
Wuhan Botanical Garden
2022
Chinese Academy of Sciences
2022
Hainan University
2022
Qingdao University of Science and Technology
2022
C99 (also known as β-CTF) is the 99 residue transmembrane C-terminal domain (residues 672–770) of amyloid precursor protein and immediate amyloid-β (Aβ) polypeptides. To test dependence structure on composition host model membranes, NMR studies were conducted both in anionic lyso-myristoylphosphatidylglycerol (LMPG) micelles a series five zwitterionic bicelle compositions involving phosphatidylcholine sphingomyelin which acyl chain lengths these lipid components varied from 14 to 24 carbons....
Abstract Temozolomide (TMZ) is widely utilized as the primary chemotherapeutic intervention for glioblastoma. However, clinical use of TMZ limited by its various side effects and resistance to chemotherapy. The present study revealed synergistic inhibition glioblastoma through combined administration perifosine. This combination therapy markedly diminished BRCA1 expression, resulting in suppression DNA repair mechanisms. Furthermore, perifosine elicited caspase-dependent apoptosis,...
We present an evaluation of a novel technique for continuous (i.e., automatic) monitoring relative cardiac output (CO) changes by long time interval analysis peripheral arterial blood pressure (ABP) waveform in humans. specifically tested the mathematical based on existing invasive and noninvasive hemodynamic data sets. With former set, we compared application to ABP waveforms obtained via radial artery catheterization with simultaneous thermodilution CO measurements 15 intensive care unit...
Bilayered detergent-lipid assemblies known as bicelles have been widely used model membranes in structural biological studies and are being explored for wider applications, including pharmaceutical use. Most to date involved the use of concentrated bicelle mixtures, such that little is about capacity bicellar mixtures be diluted without unwanted transitions nonisotropic phases. Here, different detergent/lipid explored, leading identification two families which it possible lower total...
The Notch transmembrane domain exhibits significant structural differences from that of the amyloid precursor protein.
Based on dynamic analysis of rolling bearings, the nonlinear differential equations a cylindrical roller bearing with trilobe-raceway were established and solved by GSTIFF (gear stiff) integer algorithm variable step. The influences structural parameters tolerance trilobe-raceway, working conditions bearing, outer ring installation method cage slip characteristics investigated. results show that: (i) ratio rating life would reduce when low-radius (radius raceway contour at lowest point)...
Integrins are transmembrane receptors composed of α and β subunits. Although most integrins contain β1, canonical activation mechanisms based on studies the platelet integrin, αIIbβ3. Its inactive conformation is characterized by association αIIb cytosolic domain (TM/CT) with a tilted β3 TM/CT that leads to when disrupted. We show significant structural differences between β1 in bicelles. Moreover, ‘snorkeling’ lysine at interface subunits, previously proposed regulate αIIbβ3 ion pairing...
The Notch signaling pathway is critical in development, neuronal maintenance, and hematopoiesis. An obligate step the activation of this cleavage its transmembrane (TM) domain by γ-secretase. While soluble domains have been extensively studied, little has done to characterize TM flanking juxtamembrane (JM) segments. Here, we present results nuclear magnetic resonance (NMR) studies human Notch1 TM/JM domain. largely α-helical. JM segments do not adopt regular secondary structure, they...
Hydrogen bonds play critical roles in protein structure, stability, and function. Conventionally, hydrogen are mainly determined by X-ray crystallography NOE-based NMR spectroscopy indirect manners. In recent years, it was demonstrated that can be directly detected through measurements of trans-hydrogen-bond scalar coupling constants. Here we report across hydrogen-bond protium/deuterium isotope effects a 35 kDA observed with the isotopomer-selective TROSY technique (Liu et al. J. Biomol....
Computationally designed transmembrane α-helical peptides (CHAMP) have been used to compete for helix–helix interactions within the membrane, enabling ability probe activation of integrins αIIbβ3 and αvβ3. Here, this method is extended towards design CHAMP that inhibit association α5β1 (TM) domains, targeting Ala–X3–Gly motif α5. Our previous algorithm was performed alongside a new workflow implemented widely Rosetta molecular modeling suite. Peptides from each computational approach...
This study generated and analyzed complete plastome internal transcribed spacer (ITS) data of 46 Lactuca species, 13 African endemic (AE) 15 species from eight related genera in Lactucinae. The new nuclear ITS sequences were then used to reconstruct the phylogenetic relationships species. whole-plastome estimate divergence time ancestral area reconstruction identified major lineages. results showed that are generally similar size, Guanine Cytosine (GC) content, gene structure, categories,...
There remains a need for new non-ionic detergents that are suitable use in biochemical and biophysical studies of membrane proteins. Here we explore the properties n-dodecyl-β-melibioside (β-DDMB) micelles as medium Melibiose is d-galactose-α(1→6)-d-glucose. Light scattering showed β-DDMB micelle to be roughly 30 kDa smaller than formed by commonly used n-dodecyl-β-maltoside (β-DDM). stabilized diacylglycerol kinase (DAGK) against thermal inactivation. Moreover, activity assays conducted...
Abstract The configuration and hydrogen‐bonding network of side‐chain amides in a 35 kDa protein were determined by measuring differential trans‐hydrogen‐bond H/D isotope effects using the isotopomer‐selective (IS)‐TROSY technique, which leads to reliable recognition correction erroneous rotamers that are frequently found structures. First, two‐bond on carbonyl 13 C′ shifts, defined as Δ 2 C′(ND)= C′(ND E )‐ Z ), provide means for assignment amides, because environmental (hydrogen bonds...
Loss of β1 integrin expression inhibits renal collecting-system development. Two highly conserved NPXY motifs in the distal tail regulate function by associating with phosphtyrosine binding (PTB) proteins, such as talin and kindlin. Here, we define roles these two tyrosines development delineate structural determinants using nuclear magnetic resonance (NMR). Mice carrying alanine mutations have moderate developmental abnormalities relative to β1-null mice. Phenylalanine did not affect but...
Dihydroneopterin aldolase (DHNA) catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin and also epimerization DHNP 7,8-dihydromonapterin. Previously, we determined crystal structure Staphylococcus aureus DHNA (SaDHNA) in complex with substrate analogue neopterin (NP). We showed that Escherichia coli (EcDHNA) SaDHNA have significantly different binding catalytic properties by biochemical analysis. On basis these structural functional data, proposed a mechanism...
Kidney collecting system development requires integrin-dependent cell-extracellular matrix interactions. Integrins are heterodimeric transmembrane receptors consisting of α and β subunits; crucial integrins in the kidney express β1 subunit. The cytoplasmic tail has two NPxY motifs that mediate functions by binding to signaling scaffolding molecules. Talins, proteins bind membrane proximal motif, proposed activate link them actin cytoskeleton. We have defined role talin motif developing mice...
KLHL-12 is a substrate specific adapter protein for Cul3-Ring ligase complex. It member of the Kelch β-propeller domain subclass Cullin-Ring recognition domains. This E3 ubiquitin complex has many activities, including acting as negative regulator Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis Dvl3/Dsh3. also known to mediate polyubiquitination dopamine D4 receptor (D4.2), KHSRP, that involved in IRES translation, Sec31, which endoplasmic reticulum–Golgi...
Background Studies have shown that longer leukocyte telomere length (LTL) is significantly associated with increased risk of meningioma. However, there limited evidence concerning the causal association LTL benign and malignant meningiomas or location tumors. Methods We used three datasets from different sources, designated by name sample size as LTL-78592, LTL-9190, LTL-472174. The linkage disequilibrium score (LDSC) was to explore between utilized two-sample bidirectional Mendelian...
6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the transfer of pyrophosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), which follows an ordered bi-bi kinetic mechanism with binding enzyme first. HPPK undergoes dramatic conformational changes during its catalytic cycle as revealed by X-ray crystallography, and are essential for enzymatic catalysis shown site-directed mutagenesis biochemical crystallographic analysis mutants. However, dynamic properties have not...
ABSTRACT SpoIIID is evolutionarily conserved in endospore-forming bacteria, and it activates or represses many genes during sporulation of Bacillus subtilis . An monomer binds DNA with high affinity moderate sequence specificity. In addition to a predicted helix-turn-helix motif, has C-terminal basic region that contributes binding. The nuclear magnetic resonance (NMR) solution structure complex revealed does indeed have domain novel helical extension. Residues both these regions interact...