Astragalus membranaceus is a medicinal and edible species in China, with variety of biological activities. This study evaluated the reuse potential A. waste as source food antioxidants. Antioxidant antifungal activities flavonoids, polysaccharides, saponins from stems leaves were evaluated. Results showed that inhibition rate flavonoids on six tested fungi reaches 100 % at concentration 5 mg/mL, antioxidant test demonstrated satisfactory activity. On this basis, an extremely economical...

An effective method for obtaining resistant transgenic plants is to induce RNA silencing by expressing virus-derived dsRNA in and this has been successfully implemented the generation of different plant lines many viruses.Inverted repeats partial Tobacco mosaic virus (TMV) movement protein (MP) gene Cucumber (CMV) replication (Rep) were introduced into expression vector recombinant plasmids transformed Agrobacterium tumefaciens. Agrobacterium-mediated transformation was carried out three...

Inertial microfluidics has demonstrated its ability to focus particles in a passive and straightforward manner. However, achieving flow-rate- particle-size-insensitive focusing large-dimension channels with simple design remains challenging. In this study, we developed spiral microfluidic channel achieve inertial focusing. By designing unique "big buffering area" "small the microchannel, observed stabilization acceleration of secondary flow. Our optimized allowed for efficient (>99.9%) 15 μm...

Abstract Background Forsythia suspensa (Thunb.) Vahl is a valuable ornamental and medicinal plant. Although the nuclear chloroplast genomes of F. have been published, its complete mitochondrial genome sequence has yet to be reported. In this study, genomic DNA yellowish leaf material was extracted, sequenced by using mixture Illumina Novaseq6000 short reads Oxford Nanopore PromethION long reads, sequencing data were assembled annotated. Result The obtained in length 535,692 bp with circular...

Isolation and manipulation of single cells play a crucial role in drug screening. However, previously reported single-cell screening lacked multiple-dose concentration gradient studies, which limits their ability to predict performance accurately. To solve this problem, we constructed multiconcentration generator Tai Chi-spiral mixer can accelerate solution mixing short time produce linear gradient. Later, combined with capture array was adopted investigate the effects or doses...

Microfluidic technologies enabling the control of secondary flow are essential for successful separation blood cells, a process that is beneficial wide range medical research and clinical diagnostics. Herein, we introduce dimension-confined microfluidic device featuring double-spiral channel designed to regulate flows, thereby high-throughput isolation plasma extraction. By integrating sequence micro-obstacles within microchannels, stable enhanced Dean-like across each loop can be generated....

Tomato zonate spot virus (TZSV), a new species of genus Tospovirus, caused significant losses in yield and problems quality many important vegetables ornamentals Southwest China posed serious threat to economic crops for the local farmers. A convenient reliable method was urgently needed rapid detection surveillance TZSV. The nucleocapsid protein (N) TZSV expressed Escherichia coli purified, used as antigen immunize BALB/c mice. Three monoclonal antibodies (mAbs) 3A2, 5D2 5F7 against were...

Abstract The black cutworm, Agrotis ipsilon (Hufnagel), a seasonal migrant and prolific generalist, can feed on nearly all vegetables grain crops, causing considerable economic impacts global scale. Given its cryptic nature, A. management has been extremely challenging. Chitin synthase (CHS), key enzyme involved in chitin biosynthetic pathway crucially important for the growth development of insects, is molecular target synthesis inhibitors, group broad-spectrum insecticides that compatible...

Although microfluidic devices have made remarkable strides in blood cell separation, there is still a need for further development and improvement this area. Herein, we present novel ultralow aspect ratio (H/W = 1:36) spiral channel device with ordered micro-obstacles sheathless flow-rate-insensitive separation. By introducing into the microchannels, reduced magnitude fluctuations secondary flow across different loops can be obtained through geometric confinement. As result, unique Dean-like...

The harpin protein Hpa1 has various beneficial effects in plants, such as promoting plant growth and inducing pathogen resistance. Our previous study found that could significantly alleviate the mosaic symptoms of tobacco virus (TMV) Pinellia ternata, indicating can effectively stimulate Here, potential mechanism disease resistance field applicability against TMV P. ternata were further investigated. results showed 15 µg ml −1 had stronger antiviral activity than control, its protective...

Scutellaria baicalensis Georgi. (Chinese skullcap or Huang-qin) is an extremely crucial medicinal plant in the Labiate family, and color of its flowers naturally appears purple. However, during long-term cultivation S. baicalensis, very few plants also present white purple-red flower colors under same ecological conditions. complex metabolic transcriptional networks underlying formation white, purple-red, purple remain largely unclarified. To gain insight into this issue, we conducted...

The field of inertial microfluidics has been significantly advanced in terms application to fluid manipulation for biological analysis, materials synthesis, and chemical process control. Because their superior benefits such as high-throughput, simplicity, accurate manipulation, designs incorporating channel geometries generating Dean vortexes helical have studied extensively. However, existing technologies not by designing low-aspect-ratio microchannels produce multi-vortexes. In this study,...

Scutellaria baicalensis Georgi (SB), a plant of the Lamiaceae family, contains flavonoids with potent human health benefits. The full mechanistic details and regulatory networks related to biosynthesis these compounds in SB have been focus recent research but are still fragmented. Similarly, complete account metabolites produced, specifically flavonoids, their distribution different parts is incomplete. To provide more picture, herein we explored differentially expressed genes underground...

With the continuous development of cancer therapy, conventional animal models have exposed a series shortcomings such as ethical issues, being time consuming and having an expensive cost. As alternative method, microfluidic devices shown advantages in drug screening, which can effectively shorten experimental time, reduce costs, improve efficiency, achieve large-scale, high-throughput accurate analysis. However, most these technologies are established for narrow-range drug-concentration...

Introduction:Astragalusmembranaceus Fisch. ex Bunge is a traditional botanical drug with antibacterial, antioxidant, antiviral, and other biological activities. In the process of industrialization A. membranaceus, most aboveground stems leaves are discarded without resource utilization except for small amount low-value applications such as composting. This study explored antibacterial activity membranaceus stem leaf extracts to evaluate its potential feed antibiotic substitute. Materials...