A5078741599- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Diphtheria, Corynebacterium, and Tetanus
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- COVID-19 diagnosis using AI
- Plant Pathogenic Bacteria Studies
- Infections and bacterial resistance
- Advanced X-ray and CT Imaging
- Vibrio bacteria research studies
- Tuberculosis Research and Epidemiology
- Veterinary medicine and infectious diseases
- Radiomics and Machine Learning in Medical Imaging
- Autoimmune and Inflammatory Disorders
- Streptococcal Infections and Treatments
- Sepsis Diagnosis and Treatment
- Bacterial biofilms and quorum sensing
- Botulinum Toxin and Related Neurological Disorders
- Urinary Tract Infections Management
- Toxin Mechanisms and Immunotoxins
- Insect behavior and control techniques
- Syphilis Diagnosis and Treatment
University of Insubria
2024-2025
Ospedale di Circolo e Fondazione Macchi
2024-2025
Sapienza University of Rome
2019-2024
Université Paris Cité
2023
Institut Pasteur
2023
Policlinico Umberto I
2023
Azienda Ospedaliero Universitaria Ospedali Riuniti
2023
From January 2019 to April 2020, 32 KPC-producing, ceftazidime-avibactam (CZA)-resistant Klebsiella pneumoniae strains were isolated in a university hospital Rome, Italy. These belonged the sequence type 512 (ST512), ST101, and ST307 high-risk clones.
A 68-year-old man had recurrent bacteremia by Klebsiella pneumoniae carbapenemase (KPC)-producing K. resistant to ceftazidime-avibactam and cefiderocol. The sequencing of a target region showed that it harbored KPC-3 variant enzyme (D179Y; KPC-31), which confers resistance restores meropenem susceptibility. patient was successfully treated with meropenem-vaborbactam.
Two mutually related pandemics are ongoing worldwide: the COVID-19 and antimicrobial resistance pandemics. This study aims to evaluate impact of on multi-drug-resistant Gram-negative bacteria (MDR-GN) bloodstream infections (BSIs) in a single intensive care unit (ICU). We conducted retrospective including patients admitted ICU, reorganized for patients' healthcare, with at least one confirmed MDR-GN BSI during 2019-2020. compared clinical microbiological features, incidence density,...
To report an outbreak of hypervirulent Klebsiella pneumoniae (hvKp) in COVID-19 patients.Prospective, observational study including consecutive patients with hvKp infections admitted to the University Hospital Pisa (Italy). Clinical data and outcome were collected. All followed-up 30 days from diagnosis infection. Mortality within infection was reported. The hypermucoviscous phenotype determined by 'string test'. Molecular typing performed on three strains collected during different periods...
Providencia stuartii is a member of the Morganellaceae family, notorious for its intrinsic resistance to several antibiotics, including last-resort drugs such as colistin and tigecycline. Between February March 2022, four-patient outbreak sustained by P. occurred in hospital Rome. Phenotypic analyses defined these strains eXtensively Drug-Resistant (XDR). Whole-genome sequencing was performed on representative resulted fully closed genomes plasmids. The were highly related phylogenetically...
Corynebacteria of the diphtheriae species complex (CdSC) can cause diphtheria in humans and have been reported from companion animals. We aimed to describe animal infection cases caused by CdSC isolates. A total 18,308 animals (dogs, cats, horses, small mammals) with rhinitis, dermatitis, nonhealing wounds, otitis were sampled metropolitan France (August 2019 August 2021). Data on symptoms, age, breed, administrative region origin collected. Cultured bacteria analyzed for tox gene presence,...
In February 2022, a critically ill patient colonized with carbapenem-resistant K. pneumoniae producing KPC-3 and VIM-1 carbapenemases was hospitalized for SARS-CoV-2 in the intensive care unit of Policlinico Umberto I hospital Rome, Italy. During 95 days hospitalization, ceftazidime/avibactam, meropenem/vaborbactam, cefiderocol were administered consecutively to treat 3 respiratory tract infections sustained by different bacterial agents. Those therapies altered resistome sequence type 512...
Cefiderocol (FDC), a siderophore-cephalosporin conjugate, is the newest option for treating infection with carbapenem-resistant gram-negative bacteria. We identified novel mechanism contributing to decreased FDC susceptibility in Klebsiella pneumoniae clinical isolates. The involves 2 coresident plasmids: pKpQIL, carrying variants of bla
ABSTRACT Background Myiases are fly larvae parasitic infections involving host tissues. In humans, myiases generally occur in people with reduced self-care capacity or living poor hygienic conditions, wounds serving as an entry point for flies. addition to direct damage, constitute a risk factor secondary bacterial infections. Case Summary An 81-year-old patient multiple comorbidities and conditions accessed the emergency room of care hospital Varese area following deterioration his clinical...
Streptococcus pyogenes (Group A Streptococcus; GAS) is a pathogen of global significance. In the pre-antibiotic era, GAS was major cause childhood morbidity and mortality, but its spread rapidly declined until mid-2010s. The continuing increase in infections, associated with expansion M1UK lineage, observed first United Kingdom (UK) and, later, globally. Here, we endeavor to assess various determinants underlying post-pandemic upsurge, focus on microbial genomic features. We performed an...
Antimicrobial therapy is important for case management of diphtheria, but knowledge on the emergence multidrug-resistance in Corynebacterium diphtheriae scarce. We report genomic features two multidrug-resistant toxigenic isolates sampled from wounds France 3 years apart. Both were resistant to spiramycin, clindamycin, tetracycline, kanamycin and trimethoprim-sulfamethoxazole. Genes ermX, cmx, aph(3’)-Ib, aph(6)-Id, aph(3’)-Ic, aadA1, dfrA15, sul1, cmlA, cmlR tet(33 ) clustered islands, one...
In this study, we investigated VIM-1-producing Escherichia coli , Klebsiella oxytoca pneumoniae Citrobacter freundii and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance carbapenem-resistant Enterobacterales large university hospital Italy. strains colonized the gut patients, with up to three different VIM-1-positive bacterial species from single rectal swab, but also caused bloodstream infection one patient.
ABSTRACT Neomycin is the first-choice antibiotic for treatment of porcine enteritis caused by enterotoxigenic Escherichia coli . Resistance to this aminoglycoside on rise after increased use neomycin due ban zinc oxide. We identified resistance determinants and plasmid contents in a historical collection 128 neomycin-resistant clinical E. isolates from Danish pig farms. All were characterized whole-genome sequencing antimicrobial susceptibility testing, followed conjugation experiments...
ABSTRACT Carbapenemase-producing Klebsiella pneumoniae represents a major public health issue globally. Isolates with resistance to the newest drugs, like ceftazidime/avibactam (CZA), are increasingly reported. In this study, we analyzed evolution of KPC-3-producing sequence type (ST) 512 K . strains isolated at three different times (hospitalization days 45, 56, and 78) from same patient, two which were observed in pericholecystic liver abscess. The K. isolates (295Kp, 304Kp, hmv-318Kp)...
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Shigella flexneri is a major health burden in low- and middle-income countries, where it leading cause of mortality associated with diarrhoea children, shows an increasing incidence among travellers men having sex men. Like all spp., S. has evolved from commensal Escherichia coli following the acquisition large plasmid pINV, which contains genes essential for virulence. Current sequence typing schemes are based on combinations chromosomal genetic loci, since pINV-encoded virulence often lost...
Syphilis is a sexually acquired chronic infection caused by Treponema pallidum and characterized variety of clinical manifestations. The secondary stage the disease results from hematogenous lymphatic dissemination treponemes after few weeks or months, it recurrent activity disease, with muco-cutaneous as well systemic Mucosal lesions range small, superficial ulcers that resemble painless aphthae to large gray plaques, they are generally associated manifestations disease. exclusive...
The first reports of carbapenem-resistant Enterobacterales in our hospital date back to 2006. In that period, few ertapenem-resistant but meropenem-susceptible Klebsiella pneumoniae isolates belonging sequence type (ST) 37 were retrieved from clinical samples. These strains produced the CTX-M-15 extended spectrum β-lactamase, OmpK35 was depleted due a nonsense mutation, and novel OmpK36 variant identified. Yet, starting 2010, carbapenemase (KPC)-producing ST512 started prevailing ST37...