A5034862984- Trace Elements in Health
- Metal complexes synthesis and properties
- Electrochemical Analysis and Applications
- Heavy Metal Exposure and Toxicity
- Nanoparticle-Based Drug Delivery
- Drug Transport and Resistance Mechanisms
- Iron Metabolism and Disorders
University of Wrocław
2023-2024
Mammalian zinc metallothionein-3 (Zn7MT3) plays an important role in protecting against copper toxicity by scavenging free Cu(II) ions or removing bound to β-amyloid and α-synuclein. While previous studies reported that Zn7MT3 reacts with form Cu(I)4Zn(II)4MT3ox containing two disulfides (ox), the precise localization of metal remained unclear. Here, we undertook comprehensive structural characterization metal-protein complexes formed reaction between using native ion mobility mass...
Mammalian metallothioneins (MTs) are small Cys-rich proteins involved in Zn(II) and Cu(I) homeostasis. They bind seven ions two distinct β- α-domains, forming Zn3Cys9 Zn4Cys11 clusters, respectively. After six decades of research, their role cellular buffering has begun to be understood recently. This is because different affinities bound the proteins' coexistence variously Zn(II)-loaded Zn4-7MT species cell. To date, it remained unclear how these mechanisms action occur differentiated...
Within the intricate landscape of proteome, approximately 30% all proteins bind metal ions. This repertoire is even larger when considering different forms a protein, known as proteoforms. Here, we propose term "metalloforms" to refer structural or functional variations protein resulting from binding various hetero- homogeneous Using human Cu(I)/Zn(II)-metallothionein-3 representative model, developed chemical proteomics strategy simultaneously differentiate and map Zn(II) Cu(I) sites. In...
Herby, the interaction of metallothioneins with commonly used Pt-based anticancer drugs – cisplatin, carboplatin, and oxaliplatin - was investigated using combined power elemental (i.e. LA-ICP-MS) molecular MALDI-TOF-MS) analytical techniques. The amount Cd Pt incorporated within protein determined for monomers dimers/oligomers formed by non-oxidative dimerization. Moreover, fluorescence spectrometry Zn2+-selective fluorescent indicator FluoZin3 employed to monitor ability release natively...