A5037240707- Antibiotic Resistance in Bacteria
- Biochemical and Structural Characterization
- Pharmaceutical and Antibiotic Environmental Impacts
- Enzyme Production and Characterization
- Pneumonia and Respiratory Infections
- Glycosylation and Glycoproteins Research
- Bacterial Infections and Vaccines
- Reproductive tract infections research
- Advanced Nanomaterials in Catalysis
- Peptidase Inhibition and Analysis
- Antibiotics Pharmacokinetics and Efficacy
- Enzyme Structure and Function
- Neonatal Health and Biochemistry
- Erythrocyte Function and Pathophysiology
- Biochemical and Molecular Research
- Infant Nutrition and Health
- Legume Nitrogen Fixing Symbiosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Algal biology and biofuel production
- RNA and protein synthesis mechanisms
- Antibiotic Use and Resistance
- Copper-based nanomaterials and applications
- Phytoplasmas and Hemiptera pathogens
- Photosynthetic Processes and Mechanisms
- Toxin Mechanisms and Immunotoxins
Banaras Hindu University
2024
University of Wrocław
2022-2024
Mayo Clinic
2024
NYU Langone Health
2022
National Institute of Immunology
2017-2022
Jawaharlal Nehru University
2022
Life Sciences Research Foundation
2022
Birla Institute of Technology, Mesra
2022
Sam Higginbottom Institute of Agriculture
2022
Medical University of South Carolina
2019-2021
The increasing prevalence of extended-spectrum β-lactamases (ESBLs) has abated therapeutic options worldwide. This study was undertaken to investigate the molecular profile and resistance patterns ESBLs among clinical isolates Escherichia coli Klebsiella pneumoniae at four tertiary care centres in India.Clinical E. K. were collected from All India Institute Medical Sciences (AIIMS), New Delhi; Jawaharlal Postgraduate Education & Research (JIPMER), Puducherry; (PGIMER), Chandigarh Christian...
The antimicrobial effects of copper ions and salts are well known, but the cuprous oxide nanoparticles (Cu2O-NPs) on staphylococcal biofilms have not yet been clearly revealed. present study evaluated Cu2O-NPs for their antibacterial antibiofilm activities against heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) S. (VISA). Nanoscaled Cu2O, generated by solution phase technology, contained Cu2O octahedral nanoparticles. Field emission electron microscopy demonstrated...
New Delhi metallo-beta-lactamase (NDM)-mediated carbapenem resistance in Pseudomonas aeruginosa and Acinetobacter baumannii is a major concern. We investigated the presence of NDM its variants P. A. at tertiary hospital North India. A total 236 isolates (130 106 baumannii) were included; 38 (29.23%) 20 (18.8%) resistant to carbapenems all them blaNDM positive. All carbapenem-resistant harbored blaNDM-1, while 12 (60%) blaNDM-2. Pulsed-field gel electrophoresis showed that 58 clonally...
ABSTRACT The bilobal lactoferrin is an approximately 76 kDa glycoprotein. It sequesters two Fe 3+ ions together with ions. C‐terminal half (residues, Tyr342–Arg689, C‐lobe) of bovine (BLF) (residues Ala1–Arg689) was prepared by limited proteolysis using trypsin. Both C‐lobe and intact BLF were saturated to 100%. them retained up nearly 85% iron at pH 6.5. At 5.0, 75% whereas protein could retain only slightly more than 60%. 4.0 both contained 25% 2.0 they left concentration 10%. structure...
The global incidence of the sexually transmitted disease gonorrhea is expected to rise due spread
Background Increasing use of colistin has led to the world-wide emergence mobile resistant gene ( mcr ). The present study aimed identify and characterise other drug-resistant genes in Klebsiella pneumoniae clinical isolates. Methods Twenty-two K. were analysed for genes, efflux pumps, virulence their biofilm forming ability. Pulsed-field gel electrophoresis (PFGE) multi-locus sequence typing (MLST) performed all mcr-1 positive S1-PFGE Southern hybridisation localisation bla NDM . Results...
The incidences of infections caused by an aerobic Gram-negative bacterium, Acinetobacter baumannii are very common in hospital environments. It usually causes soft tissue including urinary tract and pneumonia. is difficult to treat due acquired resistance available antibiotics well known. In order design specific inhibitors against one the important enzymes, peptidyl-tRNA hydrolase from baumannii, we have determined its three-dimensional structure. Peptidyl-tRNA (AbPth) involved recycling...
Surface proteins in Gram-positive bacteria are incorporated into the cell wall through a peptide ligation reaction catalyzed by transpeptidase sortase. Six main classes (A-F) of sortase have been identified which class A is meant for housekeeping functions. The prototypic (SaSrtA) from Staphylococcus aureus cleaves LPXTG-containing at scissile T-G bond and ligates protein-LPXT to terminal Gly residue nascent cross-bridge peptidoglycan lipid II precursor. Sortase-mediated ("sortagging")...
Activation of T cells upon engagement the cell antigen receptor rapidly leads to a number phosphorylation and plasma membrane recruitment events. For example, translocation phospholipase-Cγ1 (PLC-γ1) its association with transmembrane adapter protein LAT two other proteins, Gads SLP-76, are critical events in early activation process. We have previously characterized formation tetrameric LAT-Gads-SLP-76-PLC-γ1 complex by reconstitution vitro also thermodynamics tetramer formation. In current...
Lactoperoxidase (LPO, EC 1.11.1.7) is a member of the mammalian heme peroxidase family which also includes thyroid (TPO). These two enzymes have sequence homology 76%. The structure LPO known but not that TPO. In order to determine mode binding antithyroid drugs peroxidase, we determined crystal complexed with an drug, methimazole (MMZ) at 1.97 Å resolution. was isolated from caprine colostrum, purified homogeneity and crystallized 20% poly(ethylene glycol)-3350. Crystals were soaked in...
Mammalian metallothioneins (MTs) are small Cys-rich proteins involved in Zn(II) and Cu(I) homeostasis. They bind seven ions two distinct β- α-domains, forming Zn3Cys9 Zn4Cys11 clusters, respectively. After six decades of research, their role cellular buffering has begun to be understood recently. This is because different affinities bound the proteins' coexistence variously Zn(II)-loaded Zn4-7MT species cell. To date, it remained unclear how these mechanisms action occur differentiated...
Abstract Background Mammalian metallothioneins (MTs) are small (6–7 kDa), intracellular, cysteine-rich, metal-binding proteins involved, inter alia, in the homeostasis of zinc and copper, detoxification heavy metals, antioxidation against reactive oxygen species, protection DNA damage. The high cysteine content (~ 30%) MTs makes them toxic to bacterial cells during protein production, resulting low yield. To address this issue, we present for first time a combinatorial approach using...
Bovine lactoferrin, a 76-kDa glycoprotein (Ala1-Arg689) consists of two similar N- and C-terminal molecular halves with the ability to bind Fe(3+) ions. The N-terminal half, designated as N-lobe (Ala1-Arg341) half C-lobe (Tyr342-Arg689) have iron-binding properties, but resistant prolongs physiological role bovine lactoferrin in digestive tract. Here, we report crystal structure true C-lobe, which was produced by limited proteolysis using trypsin. In first step, fragments 21 kDa...
Among all approaches used for the semisynthesis of natural or chemically modified products, enzyme-assisted ligation is among most promising and dynamically developing approaches. Applying an efficient C247A mutant Oldenlandia affinis plant ligase OaAEP1 solid-phase peptide synthesis chemistry, we present chemoenzymatic a complete sequence cysteine-rich metal-binding cyanobacterial metallothionein Synechococcus A (SmtA). Zn(II) Cd(II) binding to newly synthesized SmtA showed identical...
In the tick-borne pathogens, Borreliella burgdorferi and Borrelia hermsii, c-di-GMP is produced by a single diguanylate cyclase (Rrp1). these Plz proteins (PlzA, B C) are only receptors identified to date PlzA sole receptor found in all isolates. Bioinformatic analyses suggest that has unique PilZN3-PilZ architecture with relatively uncommon xPilZ domain. Here, we present crystal structure of complex (1.6 Å resolution). This first xPilz domain be determined. two-domain structure, where each...