A5101425066- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Vitamin C and Antioxidants Research
- Hemoglobin structure and function
- Protein Structure and Dynamics
- Algal biology and biofuel production
- Erythrocyte Function and Pathophysiology
- Nitric Oxide and Endothelin Effects
- Photosynthetic Processes and Mechanisms
- Chemical Analysis and Environmental Impact
- Heat shock proteins research
- Polysaccharides Composition and Applications
- Photoreceptor and optogenetics research
- Enzyme Structure and Function
- Malaria Research and Control
- Botanical Research and Applications
- Genetic and Environmental Crop Studies
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Neonatal Health and Biochemistry
- Agricultural pest management studies
- 3D Printing in Biomedical Research
- Trace Elements in Health
- Insect and Pesticide Research
- Medical and Biological Ozone Research
- Mosquito-borne diseases and control
Banaras Hindu University
2023
Indian Institute of Technology BHU
2023
Michigan State University
2021-2023
All India Institute of Medical Sciences
2008-2021
Max Planck Institute of Biochemistry
2018-2020
Chaudhary Charan Singh Haryana Agricultural University
2020
Sharda University
2019
University of Delhi
2013-2018
Indian Institute of Technology Delhi
2016
Photosynthetic organisms possess a variety of mechanisms to achieve balance between absorbed light (source) and the capacity metabolically utilize or dissipate this energy (sink). While regulatory processes that detect changes in metabolic status/balance are relatively well studied plants, analogous pathways remain poorly characterized photosynthetic microbes. Here, we explored systemic result from alterations carbon availability model cyanobacterium Synechococcus elongatus PCC 7942 by...
The crystal structure of the complex lactoperoxidase (LPO) with its physiological substrate thiocyanate (SCN(-)) has been determined at 2.4A resolution. It revealed that SCN(-) ion is bound to LPO in distal heme cavity. observed orientation shows sulfur atom closer iron than nitrogen atom. forms a hydrogen bond water (Wat) molecule position 6'. This stabilized by two bonds Gln(423) N(epsilon2) and Phe(422) oxygen. In contrast, placement myeloperoxidase (MPO) occurs an opposite orientation,...
Isoniazid (INH) is an anti-tuberculosis prodrug that activated by mammalian lactoperoxidase and Mycobacterium tuberculosis catalase peroxidase (MtCP). We report here binding studies, enzyme assay involving INH, the crystal structure of complex bovine (LPO) with INH to illuminate properties activation as well mode diffusion interactions together a detailed structural functional comparison MtCP. The determination shows isoniazid binds LPO at substrate site on distal heme side. connected...
Heat stress adversely affects the growth and yield of faba bean crop. Accumulation ClpB/Hsp100 class proteins is a critical parameter in induction acquired heat tolerance plants. Heat-induced expression genes has been noted diverse plant species. Using primers complementary to soybean gene, we analyzed transcript profile gene leaves seedlings flowering plants pollen grains. protein accumulation was using Arabidopsis thaliana cytoplasmic Hsp101 antibodies. The levels were significantly...
The eradication of malaria remains challenging due to the complex life cycle Plasmodium and rapid emergence drug-resistant forms falciparum vivax. New, effective, inexpensive antimalarials against multiple stages parasite are urgently needed combat spread malaria. Here, we synthesized a set novel hydroxyethylamines investigated their activities in vitro vivo. All compounds tested had an inhibitory effect on blood stage P. at submicromolar concentrations, with best showing 50% concentrations...
Lactoperoxidase (LPO, EC 1.11.1.7) is a member of the mammalian heme peroxidase family which also includes thyroid (TPO). These two enzymes have sequence homology 76%. The structure LPO known but not that TPO. In order to determine mode binding antithyroid drugs peroxidase, we determined crystal complexed with an drug, methimazole (MMZ) at 1.97 Å resolution. was isolated from caprine colostrum, purified homogeneity and crystallized 20% poly(ethylene glycol)-3350. Crystals were soaked in...
Photosynthetic organisms need to balance the rate of photosynthesis with utilization photosynthetic products by downstream reactions. While such "source/sink" pathways are well-interrogated in plants, analogous regulatory systems unknown or poorly studied single-celled algal and cyanobacterial species. Towards identification energy/sugar sensors cyanobacteria, we utilized an engineered strain Synechococcus elongatus PCC 7942 that allows experimental manipulation carbon status. We conducted a...
Online Learning is part of Blended learning and a technique in which use both ordinary teaching advanced modern online teaching, materials are largely used.
Lactoperoxidase (1.11.1.7, LPO) is a mammalian heme peroxidase found in the extracellular fluids of mammals including plasma, saliva, airway epithelial lining fluids, nasal fluid, milk, tears, gastric juices, and intestinal mucosa. To perform its innate immune action against invading microbes, LPO utilizes hydrogen peroxide (H2 O2 ) to convert thiocyanate (SCN- iodide (I- ions into oxidizing compounds hypothiocyanite (OSCN- hypoiodite (IO- ). Previously determined structures complexes with...
In this paper, a planar tag antenna for UHF band RFID composed of spiral ending meandered line with inductive loop is presented.The presented novel compact ended having double sided microstrip dipoles scales down the extent and provides an upgraded conjugate impedance matching between semiconductor ASIC.This operates at 866 MHz.Here, volume 60×16×1.6 mm 3 (0.173λ×0.046λ×0.0046λ) testified.This produces impressive reflection coefficient able to access detection territory 12.6 m.The proposed...
Protein folding has been extensively studied for the past six decades by employing solution-based methods such as solubility, enzymatic activity, secondary structure analysis, and analytical like FRET, NMR, HD exchange. However, rapid analysis of process, approaches are often plagued with aggregation side reactions resulting in poor yields. In this work, we demonstrate that a bio-layer interferometry (BLI) chaperonin detection system can identify superior refolding conditions denatured...