A5047650142- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- Biochemical effects in animals
- Escherichia coli research studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genomics and Phylogenetic Studies
- Machine Learning in Bioinformatics
- Antimicrobial Peptides and Activities
- Natural product bioactivities and synthesis
- Microbial Natural Products and Biosynthesis
- Veterinary medicine and infectious diseases
- Medical and Biological Ozone Research
- Biochemical and Structural Characterization
- Infant Nutrition and Health
- Enzyme Catalysis and Immobilization
- Advanced Nanomaterials in Catalysis
- Enzyme Production and Characterization
- bioluminescence and chemiluminescence research
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
Sri Venkateswara Institute of Medical Sciences
2022
All India Institute of Medical Sciences
2012-2021
Vanderbilt University
2020
University of California, San Diego
2005
Type III protein secretion systems (T3SS) deliver effector proteins from the Gram-negative bacterial cytoplasm into a eukaryotic host cell through syringe-like, multi-protein nanomachine. Cytosolic components of T3SS include portion export apparatus, which traverses inner membrane and features opening channel, sorting complex for substrate recognition providing energetics required secretion. Two critical efficient are gate ATPase, proposed to be linked by central stalk ATPase. We present...
The incidences of infections caused by an aerobic Gram-negative bacterium, Acinetobacter baumannii are very common in hospital environments. It usually causes soft tissue including urinary tract and pneumonia. is difficult to treat due acquired resistance available antibiotics well known. In order design specific inhibitors against one the important enzymes, peptidyl-tRNA hydrolase from baumannii, we have determined its three-dimensional structure. Peptidyl-tRNA (AbPth) involved recycling...
Lactoperoxidase (1.11.1.7, LPO) is a mammalian heme peroxidase found in the extracellular fluids of mammals including plasma, saliva, airway epithelial lining fluids, nasal fluid, milk, tears, gastric juices, and intestinal mucosa. To perform its innate immune action against invading microbes, LPO utilizes hydrogen peroxide (H2 O2 ) to convert thiocyanate (SCN- iodide (I- ions into oxidizing compounds hypothiocyanite (OSCN- hypoiodite (IO- ). Previously determined structures complexes with...
This is the first structural evidence of recognition mRNA cap structures by a ribosome inactivating protein. It well known that unique structure formed at 5' end for carrying out various processes including maturation, translation initiation, and RNA turnover. The binding studies crystal determinations type 1 protein (RIP-1) from Momordica balsamina (MbRIP-1) were carried with (i) N7-methyl guanine (m7G), (ii) guanosine diphosphate (m7GDP), (iii) triphosphate (m7GTP). These compounds showed...
Peptidoglycan (PGN) consists of repeating units N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc), which are cross-linked by short peptides. It is well known that PGN forms a major cell wall component bacteria making it an important ligand for the recognition peptidoglycan proteins (PGRPs) host. The binding studies showed PGN, GlcNAc, MurNAc bind to camel PGRP-S (CPGRP-S) with affinities corresponding dissociation constants 1.3 × 10(-9), 2.6 10(-7), 1.8 10(-7) M, respectively....
Background: Needle-stick injury (NSI) incidence is a major occupational hazard amongst healthcare workers (HCWs). Methods: In this retrospective study conducted HCWs from October 2018 to 2019, we aimed know the of NSI and its root cause. At time self-reporting NSI, details were collected regarding mode injury, viral markers source vaccination status HCWs. Exposed followed up till 6 months for seroconversion. Results: A total 47 NSIs reported during period. The rate per annum was 0.13. high...
The type 1 ribosome inactivating protein from Momordica balsamina (MbRIP1) has been shown to interact with purine bases, adenine and guanine of RNA/DNA. We report here the binding structural studies MbRIP1 a pyrimidine base, cytosine; cytosine containing nucleoside, cytidine; nucleotide, cytidine diphosphate. All three compounds bound at active site dissociation constants 10-4 M-10-7 M. As reported earlier, in structure native MbRIP1, there are 10 water molecules substrate site. Upon four...
In this demonstration, we present a database over complex documents, which, in addition to structured text content, also has update information, annotations, and embedded objects. We propose new data model called spatiotemporal annotation graphs (STAG) for of composite digital objects system that shows query language efficiently effectively such database. The particular application be demonstrated is annotated MS Word PowerPoint presentations with multimedia
Ribosome Inactivating Proteins (RIPs) are capable of inhibiting protein synthesis by catalytically hydrolyzing at specific purine residues from the sarcin / ricin loop large ribosomal RNA. There two types RIPs: type 1 - RIPs (RIP-1) single polypeptide chain proteins while 2 contain chains which covalently linked a disulphide bond. Studies have indicated that stacking interactions play dominant role in ligand binding to RIPs. However, structural basis these with as well nature stack pairing...