A5103943347- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glycosylation and Glycoproteins Research
- Crystallization and Solubility Studies
- Vitamin C and Antioxidants Research
- X-ray Diffraction in Crystallography
- Nitric Oxide and Endothelin Effects
- Enzyme Structure and Function
- Peptidase Inhibition and Analysis
- Crystallography and molecular interactions
- Rice Cultivation and Yield Improvement
- Inorganic and Organometallic Chemistry
- Infant Nutrition and Health
- Porphyrin Metabolism and Disorders
- Neurological diseases and metabolism
- Antimicrobial Peptides and Activities
- Microbial Metabolism and Applications
- Climate change impacts on agriculture
- Protein Hydrolysis and Bioactive Peptides
- RNA modifications and cancer
- Agricultural Science and Fertilization
- RNA and protein synthesis mechanisms
- Soil and Land Suitability Analysis
- Microbial Metabolites in Food Biotechnology
- Biochemical and Structural Characterization
- Studies on Chitinases and Chitosanases
All India Institute of Medical Sciences
2009-2024
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
2020-2022
Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya
2012-2022
Forest Research Institute
2020
Jodhpur National University
1996
India would require around 311 Mt of food grains (cereals and pulses) during 2030 to feed 1.43 Bn people, the requirement expectedly further increase 350 MT by 2050 when India's population be 1.8 Bn. To achieve security in country, attempts need focus on both area expansion under agriculture as well rise crop productivity. Massive urbanization is putting pressure agricultural lands, resulting shrinking land holdings. The possibility agriculture, therefore, exists restoring degraded lands....
During the course of protein synthesis in cell, translation process is often terminated due to various reasons. As a result, peptidyl-tRNA molecules are released which toxic cell as well reducing availability free amino acid and tRNA for required cell. Such situation corrected by an enzyme, Pth (peptidyl-tRNA hydrolase), catalyses release peptide moieties from peptidyl-tRNAs. This means that active essential survival bacteria. In order design inhibitors PaPth (Pth Pseudomonas aeruginosa), we...
Lactoperoxidase (1.11.1.7, LPO) is a mammalian heme peroxidase found in the extracellular fluids of mammals including plasma, saliva, airway epithelial lining fluids, nasal fluid, milk, tears, gastric juices, and intestinal mucosa. To perform its innate immune action against invading microbes, LPO utilizes hydrogen peroxide (H2 O2 ) to convert thiocyanate (SCN- iodide (I- ions into oxidizing compounds hypothiocyanite (OSCN- hypoiodite (IO- ). Previously determined structures complexes with...
Peptidoglycan (PGN) consists of repeating units N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc), which are cross-linked by short peptides. It is well known that PGN forms a major cell wall component bacteria making it an important ligand for the recognition peptidoglycan proteins (PGRPs) host. The binding studies showed PGN, GlcNAc, MurNAc bind to camel PGRP-S (CPGRP-S) with affinities corresponding dissociation constants 1.3 × 10(-9), 2.6 10(-7), 1.8 10(-7) M, respectively....
Peptidyl-tRNA hydrolase (Pth) catalyzes the breakdown of peptidyl-tRNA into peptide and tRNA components. Pth from Acinetobacter baumannii (AbPth) was cloned, expressed, purified crystallized in a native unbound (AbPth-N) state bound with phosphate ion cytosine arabinoside (cytarabine) (AbPth-C). Structures AbPth-N AbPth-C were determined at 1.36 1.10 Å resolutions, respectively. The structure showed that active site is filled water molecules. In AbPth-C, present site, while cytarabine cleft...
Lactoperoxidase (LPO) belongs to mammalian heme peroxidase superfamily, which also includes myeloperoxidase (MPO), eosinophil (EPO), and thyroid (TPO). LPO catalyzes the oxidation of a number substrates including thiocyanate while TPO biosynthesis hormones. is been shown catalyze hormones indicating similar functional structural properties. The binding studies showed that 2-mercaptoimidazole (MZY) bound with dissociation constant 0.63 µM. inhibition value IC50 was 17 crystal structure...
The oxidation of 35 monosubstituted benzaldehydes by bis(2,2'-bipyridylyl)copper(II) permanganate (BBCP) in aqueous acetic acid leads to the formation corresponding benzoic acids. reaction is first order with respect BBCP. Michaelis−Menten-type kinetics were observed aldehyde concentrations. [2H]benzaldehyde (PhCDO) indicated presence a substantial kinetic isotope effect. rates meta- and para-substituted correlated terms Charton's triparametric LDR equation, whereas ortho-substituted was...
Background: Type-III Pantothenate kinase from the multi drug resistant bacteria, Acinetobacter baumannii (AbPanK) catalyzes first step of essential Coenzyme A biosynthesis pathway. AbPanK is an attractive target against bacteria since it enzyme and its structure significantly different human PanK. Methods: was cloned, expressed, purified crystallized. good quality single crystal used for X-ray intensity data collection. Dynamic light scattering done calculating hydrodynamic radii oligomeric...
The 40 kDa secretory signalling glycoprotein (SPS-40) is the first example with Trp78 in three functional orientations: (i) a resting state pinched conformation, (ii) stacked conformation when bound to hexasaccharide and (iii) an obstructive inhibited by 2-methylpentane-2,4-diol (MPD). present core of sugar-binding groove. N-acetylglucosamine (GlcNAc6) has been shown bind SPS-40. As result this, alters from native (χ1 = −65.5°, χ2,1 −78.8°, χ2,2 97.5°) −170.0°, −114.3°, 61.6°). Further...
Aegle marmelos or bael is a tree with enormous therapeutic, religious and ethnobotanical value.It highly sacred in Hindu religion worshipped as an offering to lord Shiva.It moderate size the great importance rural economy livelihood.Bael leaves are aromatic essential oil extracted from its of medicinal value.Its fruits, leaves, wood other plant parts used edibles, fodder, timber, tannins, dyes many more purposes.This valuable agroforestry obtained hardly being utilized.In this study, was...
Climate change has become a proven threat to humanity.The unprecedented increases in the climate-related phenomenon and its impacts on nature, people, animals are glaring examples of it.Water resources agriculture sector going be hit hard.The Indian Himalayan Region (IHR) is most vulnerable region climate due peculiar geographical location, threatening food nutritional security millions people whose livelihood options primarily agriculture-based.This paper reviews current status water IHR,...