A5090329856- Infant Nutrition and Health
- Glycosylation and Glycoproteins Research
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- X-ray Diffraction in Crystallography
- Enzyme Production and Characterization
- Venomous Animal Envenomation and Studies
- Computational Drug Discovery Methods
- RNA Interference and Gene Delivery
- Amino Acid Enzymes and Metabolism
- Treatment of Major Depression
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Neonatal Health and Biochemistry
- Vitamin C and Antioxidants Research
- Digestive system and related health
- Metabolism and Genetic Disorders
- Synthesis and biological activity
- Biochemical effects in animals
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
Gautam Buddha University
2015-2025
Jain University
2024
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2024
Pt. Jawahar Lal Nehru Memorial Medical College
2023
University of Calgary
2022
National Institute on Aging
2012-2018
National Institutes of Health
2011-2018
All India Institute of Medical Sciences
2004-2018
Institute on Aging
2012-2017
Cooper Medical School of Rowan University
2014
Subanesthetic doses of (R,S)-ketamine are used in the treatment neuropathic pain and depression. In rat, antidepressant effects associated with increased activity function mammalian target rapamycin (mTOR); however, is extensively metabolized contribution its metabolites to mTOR signaling unknown.Rats (n = 3 per time point) were given (R,S)-ketamine, (R,S)-norketamine, (2S,6S)-hydroxynorketamine their effect on pathway determined after 20, 30, 60 min. PC-12 pheochromocytoma cells experiment)...
SARS-CoV-2 is causative agent of COVID-19, which responsible for severe social and economic disruption globally. Lack vaccine or antiviral drug with clinical efficacy suggested that repurposing approach may provide a quick therapeutic solution to COVID-19. Nonstructural protein-15 (NSP15) encodes an uridylate-specific endoribonuclease (EndoU) enzyme, essential virus life cycle attractive target development. We have performed in silico based virtual screening FDA approved compounds targeting...
With the progress in two distinct areas of nanotechnology and aptamer identification technologies, fields have merged to what is known as nanotechnology. Aptamers varying properties biomedical field include their small size, non-toxicity, ease manufacturing, negligible immunogenicity, ability identify a wide range targets, high immobilizing capacity. Nevertheless, aptamers can utilize characteristics offered by nanomaterials like optical, magnetic, thermal, electronic become more versatile...
Pulmonary tuberculosis is the most common manifestation of tuberculosis, and to this day, sputum smear microscopy remains widely used diagnostic test in resource-limited settings despite its suboptimal sensitivity. Here we report development two DNA aptamer-based tests, namely aptamer linked immobilized sorbent assay (Aptamer ALISA) electrochemical sensor (ECS), for direct detection a TB biomarker HspX sputum. First compared performance Aptamer ALISA with anti-HspX polyclonal antibody-based...
The novel Coronavirus disease 2019 (COVID-19) is potentially fatal and caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2). Due to the unavailability of any proven treatment or vaccination, outbreak COVID-19 wreaking havoc worldwide. Hence, there an urgent need for therapeutics targeting SARS-CoV-2. Since, botanicals are important resource several efficacious antiviral agents, natural compounds gaining significant attention treatment. In present study, methyltranferase (MTase)...
Aim: This study aimed to screen the potential phytochemicals derived from Asparagus racemosus (Shatavari) against Thymidylate Kinase (TMPK) and D9 decapping enzyme, which is vital target of monkeypox virus helps in host-- pathogen interaction mechanism, using integrated docking, QSAR analysis, a molecular dynamics approach. Background: The Monkeypox Virus (MPXV) recently emerging outbreak with ongoing infection cases. Drugs vaccines for smallpox are being used contain it. However, no...
The crystal structure of the complex lactoperoxidase (LPO) with its physiological substrate thiocyanate (SCN(-)) has been determined at 2.4A resolution. It revealed that SCN(-) ion is bound to LPO in distal heme cavity. observed orientation shows sulfur atom closer iron than nitrogen atom. forms a hydrogen bond water (Wat) molecule position 6'. This stabilized by two bonds Gln(423) N(epsilon2) and Phe(422) oxygen. In contrast, placement myeloperoxidase (MPO) occurs an opposite orientation,...
Isoniazid (INH) is an anti-tuberculosis prodrug that activated by mammalian lactoperoxidase and Mycobacterium tuberculosis catalase peroxidase (MtCP). We report here binding studies, enzyme assay involving INH, the crystal structure of complex bovine (LPO) with INH to illuminate properties activation as well mode diffusion interactions together a detailed structural functional comparison MtCP. The determination shows isoniazid binds LPO at substrate site on distal heme side. connected...
Peptidoglycan recognition proteins (PGRPs) are involved in the of pathogen-associated molecular patterns. The well known patterns include LPS from Gram-negative bacteria and lipoteichoic acid (LTA) Gram-positive bacteria. In this work, crystal structures two complexes short form camel PGRP (CPGRP-S) with LTA determined at 1.7- 2.1-Å resolutions, respectively, reported. Both compounds were held firmly inside complex formed four CPGRP-S molecules designated A, B, C, D. binding cleft is located...
Severe Acute Respiratory Syndrome Corona Virus-2 (SARS-CoV2) is responsible for fetal pneumonia called COVID19. SARS-CoV2 emerged in Wuhan, Hubei Province of China December 2019. The COVID19 pandemic has now gripped the entire world with more than 70 million cases and over 1.5 deaths so far. There no treatment option term a drug or vaccine currently available. Therefore repurposing may only provide quick method utilizing existing drugs therapeutic option. virus genome contains several...