A5016416693- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Legionella and Acanthamoeba research
- Ethnobotanical and Medicinal Plants Studies
- Infectious Diseases and Tuberculosis
- Bacterial Identification and Susceptibility Testing
- Natural Compound Pharmacology Studies
- Biosensors and Analytical Detection
- Digital Imaging for Blood Diseases
- Ocular Surface and Contact Lens
- Essential Oils and Antimicrobial Activity
- Parasites and Host Interactions
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Amoebic Infections and Treatments
- Cancer therapeutics and mechanisms
- Salivary Gland Disorders and Functions
- Vibrio bacteria research studies
- Parasitic Infections and Diagnostics
- Computational Drug Discovery Methods
- Traumatic Brain Injury and Neurovascular Disturbances
- Prion Diseases and Protein Misfolding
LRS Institute of Tuberculosis and Respiratory Diseases
2015-2025
Santosh University
2018
M. P. Shah Medical College
2013
Pulmonary tuberculosis is the most common manifestation of tuberculosis, and to this day, sputum smear microscopy remains widely used diagnostic test in resource-limited settings despite its suboptimal sensitivity. Here we report development two DNA aptamer-based tests, namely aptamer linked immobilized sorbent assay (Aptamer ALISA) electrochemical sensor (ECS), for direct detection a TB biomarker HspX sputum. First compared performance Aptamer ALISA with anti-HspX polyclonal antibody-based...
Abstract Acanthamoeba spp. can cause amoebic keratitis (AK). Chlorhexidine is effective for AK treatment as monotherapy, but with a relative failure on drug bioavailability in the deep corneal stroma. The combination of chlorhexidine and propamidine isethionate recommended current treatment. However, effectiveness depends parasite virulence strains. This study aims to determine potential Garcinia mangostana pericarp extract α-mangostin against triangularis , well infection. minimal...
Intestinal parasites predominantly coccidian are a common cause for diarrhea in human immunodeficiency virus (HIV)-positive patients.The study was conducted during January 2009-December 2010. A total of 1,088 stool samples from 544 seropositive HIV positive cases were examined microscopically ova and cyst using wet mount preparations stained smears. Out patients, 343 had prolonged more than 4 weeks, 57 acute lesser 7 days 144 asymptomatic who attended out-patient department; included this...
Acanthamoeba spp. are pathogenic microorganisms linked to severe infections in humans and animals, requiring a deeper understanding of their encystation process for effective diagnostics research. This study focused on developing novel encystment medium induce synchronized efficiently rapidly. The employed response surface methodology with central composite design optimize the formulation. key components included Tris-HCl, NaCl, glucose, MgCl2. optimized liquid was spray-dried produce...
Purpose: There is scarcity of prevalence data multi-drug–resistant tuberculosis (MDR-TB) and common mutations responsible in North India. This study aimed to detect MDR-TB among suspects from Delhi mutation patterns using GenoType MTBDRplus assay. Materials Methods: All MDR five districts New were referred the laboratory 1st October 2011 31st December 2012 as per criterion defined by Programmatic Management Drug Resistant Tuberculosis (PMDT). assay was performed on 2182 samples or cultures...
The need for an accurate, rapid, simple and affordable point-of-care (POC) test Tuberculosis (TB) that can be implemented in microscopy centers other peripheral health-care settings the TB-endemic countries remains unmet. This manuscript describes preliminary results of a new prototype rapid lateral flow TB based on detection antibodies to immunodominant epitopes (peptides) derived from carefully selected, highly immunogenic M. tuberculosis cell-wall proteins. Peptide selection was initially...
A total of 312 sputum samples from pediatric patients presumptive multidrug resistant tuberculosis were tested for the detection drug resistance using GenoTypeMTBDRplus assay. 193 (61.8%) smear positive and 119 (38.1%) negative by Ziehl-Neelsen staining. Line probe assay (LPA) was performed 208 samples/cultures (193 15 cultures samples). Valid results obtained 198 tests. Of these, 125/198 (63.1%) sensitive to both rifampicin (RIF) isoniazid (INH). 73/198 (36.9%) at least INH/RIF, out which...
The present study was conducted to evaluate the performance of Xpert(®) MTB/RIF assay and compare Xpert results with solid MGIT 960 liquid culture system. A total 134 patients who had failed Category I or II regimen were recruited for evaluation. correctly identified all Mycobacterium tuberculosis isolates. sensitivity specificity detection rifampicin resistance respectively 98.2% 97.0% when compared results.
This study aimed to analyze the trends of tuberculosis (TB) disease, drugs susceptibility patterns in geriatric TB over a period three years (from 2010 2012).
India has the highest burden of Tuberculosis (TB) and multidrug-resistant TB (MDR-TB) worldwide. Innovative technology is need hour to identify these cases that remain either undiagnosed or inadequately diagnosed due unavailability appropriate tools at primary healthcare settings. We developed evaluated 3 kits, namely 'TB Detect' (containing BioFM-Filter device), Concentration Transport' Trans-Filter device) DNA Extraction' kits. These kits enable bio-safe equipment-free concentration sputum...
Nowadays, nontuberculous mycobacteria (NTM) often cause pulmonary and extrapulmonary disease. Species identification of NTM determines the line treatment management The routine diagnostic methods, i.e., smear microscopy biochemical identification, are tedious time consuming not all laboratories can perform these tests on a basis. A PCR targeting hsp65 gene was implemented using standard strains applied to 109 clinical isolates. PCR-amplified product subjected restriction enzyme analysis...
With the introduction of novel molecular techniques that rely on rifampicin (RIF) susceptibility, resistance to isoniazid (INH) or other first-line drugs remains undetected. Such patients are prescribed antituberculosis therapy and RIF monodrug during continuation phase, which may lead therapeutic failure emergence multidrug resistance. We aimed study INH among RIF-susceptible Mycobacterium tuberculosis (MTB) isolates from retreatment patients.The Drug Susceptibility Testing data for four...
Nontuberculous mycobacteria (NTM) infections are an emerging global health concern with increasing incidence. Conventional identification methods for NTM species in clinical settings prone to errors. This study evaluates a newer method, polymerase chain reaction-restriction enzyme analysis (PCR-REA) of the rpoB gene, identification. The identified samples using conventional biochemical techniques and compared results PCR-REA gene. cross-sectional was conducted at tertiary health-care center...
Background Near-patient access to appropriate tests is a major obstacle for the efficient diagnosis of tuberculosis (TB) and associated drug resistance. Methods We recently developed “TB Concentration & Transport” kit bio-safe, ambient-temperature transportation dried sputum on Trans -Filter, DNA Extraction” extraction from -Filter determining resistance by sequencing. In present study, we evaluated compatibility Kit-extracted with Hain's line probe assays (LPAs), which are endorsed...