A5000834958- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Bacterial Identification and Susceptibility Testing
- Diagnosis and treatment of tuberculosis
- Pleural and Pulmonary Diseases
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Bacterial Infections and Vaccines
- Biosensors and Analytical Detection
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Amoebic Infections and Treatments
- Extracellular vesicles in disease
- Aquaculture disease management and microbiota
- Pancreatic and Hepatic Oncology Research
- Viral Infectious Diseases and Gene Expression in Insects
- DNA and Nucleic Acid Chemistry
- Drug Transport and Resistance Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Rabies epidemiology and control
- Medical Imaging and Pathology Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- IgG4-Related and Inflammatory Diseases
- HIV/AIDS drug development and treatment
Post Graduate Institute of Medical Education and Research
2019-2025
Translational Health Science and Technology Institute
2016-2023
Institute of Post Graduate Medical Education and Research
2022
All India Institute of Medical Sciences
2005-2012
Department of Biotechnology
2005-2012
Tuberculous meningitis (TBM) is the most devastating form of and prompt diagnosis holds key to its management. Conventional microbiology has limited utility nucleic acid-based methods have not been widely accepted for various reasons. In view paucibacillary nature cerebrospinal fluid (CSF) recent demonstration free Mycobacterium tuberculosis DNA in clinical specimens, present study was designed evaluate CSF 'filtrates' TBM using PCR. One hundred sixty-seven samples were analysed from...
Background Tuberculous meningitis (TBM) is the most common form of neurotuberculosis and fifth extrapulmonary TB. Early diagnosis prompt treatment are cornerstones effective disease management. The accurate TBM poses a challenge due to an extensive differential diagnosis, low bacterial load paucity cerebrospinal fluid (CSF) especially in children. Methodology/Principal Findings We describe utility ELISA qPCR for detection Mycobacterium tuberculosis (M. tb) proteins (GlcB, HspX, MPT51, Ag85B...
Abstract Background We recently described the utility of ‘TB Concentration & Transport’ kit for bio-safe, ambient-temperature transport dried-sputum on Trans-Filter, and DNA Extraction’ extraction from Trans-Filter early diagnosis drug-resistant Tuberculosis (TB). This study aimed to assess feasibility compatibility these kits with Line Probe Assay (LPA) under National Elimination Programme (NTEP) settings. Methods Presumptive pulmonary TB/MDR-TB/XDR-TB patients (n=8491) who attended...
The prompt diagnosis of smear-negative cases is a prerequisite to controlling tuberculosis (TB). Several new laboratory approaches, including nucleic acid amplification (NAA), are being evaluated in various disease settings meet this challenge. However, NAA needs simplification before it widely accepted. Furthermore, supporting smear result improves confidence and reliability PCR. In context, an asymmetric devR PCR assay using two molecular beacon probes for visual or fluorimetric end-point...
Sputum culture conversion in pulmonary multidrug-resistant tuberculosis (MDR-TB) is important to make treatment-related decisions and prevent transmission of disease.To identify factors associated with sputum conversion, determine time the impact on successful treatment outcomes MDR-/rifampicin (RMP) resistant TB.Retrospective analysis data from cards registers MDR-/RMP-resistant patients initiated under India's Revised National TB Control Programme Delhi, West Bengal Kerala January 2009...
Tuberculous meningitis (TBM) is the most devastating manifestation of extra-pulmonary tuberculosis. About 33% TBM patients die due to very late diagnosis disease. Conventional diagnostic methods based on signs and symptoms, cerebrospinal fluid (CSF) smear microscopy or liquid culture suffer from either poor sensitivity long turnaround time (up 8 weeks). Therefore, in order manage disease efficiently, there an urgent unmet need for a rapid reliable test.In current study, address challenge...
Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified patients estimated have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out evaluate utility high-resolution melt curve analysis (HRM) for rapid and direct detection MDR-TB Mycobacterium sputum samples. A reference plasmid library first generated most frequently observed mutations resistance-determining regions rpoB, katG, an inhA promoter used...
The successful management of tuberculosis (TB) requires efficient diagnosis and treatment. Further, the increasing prevalence drug-resistant TB highlights urgent need to develop novel inhibitors against both drug-susceptible forms disease. Malate synthase (MS), an enzyme glyoxylate pathway, plays a vital role in mycobacterial persistence, therefore it is considered as attractive target for anti-TB drug development. Recent studies have also ascribed adhesin function MS established potent...
Abdominal tuberculosis (ATB) continues to pose a major diagnostic challenge for clinicians due its nonspecific clinical presentation, variable anatomical location and lack of sensitive tools. In spite the development several assays till date; no single test has proved be adequate ATB diagnosis. this study, we first time report detection circulating cell-free Mycobacterium (M. tuberculosis) DNA (cfMTB-DNA) in ascitic fluid (AF) samples utility Sixty-five AF were included study processed...
This study was designed to assess the vital issue of gene target length and PCR assay performance in relation detection Mycobacterium tuberculosis clinical specimens. Two assays that amplify fragments varying lengths from devR M. were evaluated. Using DNA 'short-length' detected 250-500 genome equivalents vs. 500-1,000 by 'long-length' assay. In comparison a highly sensitive smear microscopy test (universal sample processing smear), sensitivity 97.8% 69.9% sputum specimens (n=506) patients...
India has the highest burden of Tuberculosis (TB) and multidrug-resistant TB (MDR-TB) worldwide. Innovative technology is need hour to identify these cases that remain either undiagnosed or inadequately diagnosed due unavailability appropriate tools at primary healthcare settings. We developed evaluated 3 kits, namely 'TB Detect' (containing BioFM-Filter device), Concentration Transport' Trans-Filter device) DNA Extraction' kits. These kits enable bio-safe equipment-free concentration sputum...
Background: Chinese Hamster Ovary cells (CHO) are the most preferred host to meet increasing demand for high quality 'human-like' complex biologics production, but is faced with challenge of achieving yield at an affordable price.Secreted proteins critically impact cell growth and product quantity integral part secretome packaged microvesicles.In spite numerous efforts characterize spent-media proteome, none have identified specific contribution microvesicles, necessitating further...
Direct smear microscopy of sputum forms the mainstay TB diagnosis in resource-limited settings. Stained slides can serve as a ready-made resource to transport for molecular drug susceptibility testing. However, bio-safety is major concern during sputum/stained and laboratory workers engaged processing Mycobacterium tuberculosis infected specimens. In this study, bio-safe USP (Universal Sample Processing) concentration-based method (Bio-safe method) was assessed on 87 M. culture positive...