- Heat shock proteins research
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Agricultural pest management studies
- Genetic and Environmental Crop Studies
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Insect and Pesticide Research
- Cell Image Analysis Techniques
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Plant responses to elevated CO2
- Botanical Research and Chemistry
- Protein Structure and Dynamics
University of Alberta
2019-2021
University of Delhi
2011-2018
With growing populations and pressing environmental problems, future economies will be increasingly plant-based. Now is the time to reimagine plant science as a critical component of fundamental science, agriculture, stewardship, energy, technology healthcare. This effort requires conceptual technological framework identify map all cell types, comprehensively annotate localization organization molecules at cellular tissue levels. framework, called Plant Cell Atlas (PCA), for understanding...
ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an important chaperone protein in rice. Cellular expression of OsClpB-cyt transcript governed by heat stress, metal and developmental cues. Transgenic rice plants produced with 2 kb promoter driving Gus reporter gene showed heat- metal-regulated vegetative tissues constitutive calli, flowering tissues, embryonal half seeds. Rice seedlings regenerated fragment deletion its canonical shock element sequence (HSE(-273 to -280)) not only inducibility...
Binding of heat shock factors (Hsfs) to elements (HSEs) leads transcriptional regulation genes. Genome-wide, 953 rice genes contain perfect-type, 695 gap-type and 1584 step-type HSE sequences in their 1-kb promoter region. The genome contains 13 class A, eight B four C Hsfs (OsHsfs) has OsHsf26 (which is variant type) Chemical cross-linking analysis vitro synthesized OsHsf polypeptides showed formation homotrimers OsHsfA2c, OsHsfA9 OsHsfB4b proteins. with oligonucleotide probes containing...
Heat stress adversely affects the growth and yield of faba bean crop. Accumulation ClpB/Hsp100 class proteins is a critical parameter in induction acquired heat tolerance plants. Heat-induced expression genes has been noted diverse plant species. Using primers complementary to soybean gene, we analyzed transcript profile gene leaves seedlings flowering plants pollen grains. protein accumulation was using Arabidopsis thaliana cytoplasmic Hsp101 antibodies. The levels were significantly...
Abstract Background Understanding developmental processes requires the unambiguous identification of cells and tissues, selective manipulation properties those tissues. Both requirements can most efficiently be satisfied through use GAL4/GFP enhancer‐trap lines. No such lines, however, have been characterized for study early leaf development in Columbia‐0 reference genotype Arabidopsis. Results Here we address this limitation by identifying characterizing a set lines background specific...
Abstract Understanding developmental processes requires the unambiguous identification of cells and tissues, selective manipulation properties those tissues. Both requirements can most efficiently be satisfied through use GAL4/GFP enhancer-trap lines. No such lines are available, however, for study leaf development in Columbia-0 reference genotype Arabidopsis. Here we address this limitation by identifying characterizing a set background specific labeling tissues during development, targeted...