A5018077070- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Diatoms and Algae Research
- Microbial bioremediation and biosurfactants
- Photoreceptor and optogenetics research
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Electrohydrodynamics and Fluid Dynamics
- Gene Regulatory Network Analysis
- Microbial Metabolic Engineering and Bioproduction
- Mesoporous Materials and Catalysis
- Evolution and Genetic Dynamics
- Metabolomics and Mass Spectrometry Studies
- bioluminescence and chemiluminescence research
- Hybrid Renewable Energy Systems
- Optical Imaging and Spectroscopy Techniques
- Graphene and Nanomaterials Applications
- Slime Mold and Myxomycetes Research
- Protist diversity and phylogeny
- Biochemical and Structural Characterization
- Adsorption and biosorption for pollutant removal
- Evolutionary Game Theory and Cooperation
- biodegradable polymer synthesis and properties
- Vibrio bacteria research studies
Michigan State University
2019-2024
Michigan United
2023-2024
University of Minnesota
2015-2021
Minneapolis Institute of Arts
2018
University of Minnesota System
2018
Biotechnology Institute
2017
Twin Cities Orthopedics
2017
John Wiley & Sons (United States)
2016
Hudson Institute
2016
Photosynthetic organisms possess a variety of mechanisms to achieve balance between absorbed light (source) and the capacity metabolically utilize or dissipate this energy (sink). While regulatory processes that detect changes in metabolic status/balance are relatively well studied plants, analogous pathways remain poorly characterized photosynthetic microbes. Here, we explored systemic result from alterations carbon availability model cyanobacterium Synechococcus elongatus PCC 7942 by...
An adsorbent silica biogel material was developed via gel encapsulation of Pseudomonas sp. NCIB 9816-4, a bacterium that degrades broad spectrum aromatic pollutants. The matrix synthesized using precursors methyltrimethoxysilane and tetramethoxysilane to maximize the adsorption capacity while maintaining highly networked porous microstructure. encapsulated bacteria enhanced removal rate for an chemical mixture. Repeated use over four cycles conducted demonstrate could be maintained with...
The disruption of bacterial signaling (quorum quenching) has been proven to be an innovative approach influence the behavior bacteria. In particular, lactonase enzymes that are capable hydrolyzing N- acyl homoserine lactone (AHL) molecules used by numerous bacteria, were reported inhibit biofilm formation, including those freshwater microbial communities. However, insights and tools currently lacking characterize, understand explain effects signal on complex Here, we produced silica capsules...
Abstract Whole cell biocatalysts can perform numerous industrially-relevant chemical reactions. While they are less expensive than purified enzymes, whole cells suffer from inherent reaction rate limitations due to transport resistance imposed by the membrane. Furthermore, it is desirable immobilize enable ease of separation mixture. In this study, we used a layer-by-layer (LbL) self-assembly process create microbial exoskeleton which, simultaneously immobilized, protected, and enhanced...
Synechococcus elongatus PCC 7942 is a model cyanobacterium for study of the circadian clock, photosynthesis, and bioproduction chemicals, yet nearly 40% its gene identities functions remain unknown, in part due to limitations existing genetic toolkit. While classical techniques genes (e.g., deletion or mutagenesis) can yield valuable information about absence associated protein, there are limits these approaches, particularly essential genes. Herein, we developed tool inducible degradation...
Photosynthetic organisms need to balance the rate of photosynthesis with utilization photosynthetic products by downstream reactions. While such "source/sink" pathways are well-interrogated in plants, analogous regulatory systems unknown or poorly studied single-celled algal and cyanobacterial species. Towards identification energy/sugar sensors cyanobacteria, we utilized an engineered strain Synechococcus elongatus PCC 7942 that allows experimental manipulation carbon status. We conducted a...
Industrial application of encapsulated bacteria for biodegradation hydrocarbons in water requires mechanically stable materials. A silica gel encapsulation method was optimized Pseudomonas sp. NCIB 9816-4, a bacterium that degrades more than 100 aromatic hydrocarbons. The design process focused on three aspects: (i) mechanical property enhancement; (ii) cytocompatibility; and (iii) reduction the diffusion barrier gel. Mechanical testing indicated compressive strength at failure (σf ) elastic...
Alginate encapsulation matrices selectively retained hydrogen producing biomass, achieving reliable high-rate production of at various operating conditions.
There has been substantial recent interest in the promise of sustainable, light-driven bioproduction using cyanobacteria, including developing efforts for microbial mixed autotroph/heterotroph communities, which could provide useful properties, such as division metabolic labor. However, building stable mixed-species communities sufficient productivity remains a challenge, partly due to lack strategies synchronizing and coordinating biological activities across different species. To address...
Abstract Synergistical bacterial species can perform more varied and complex transformations of chemical substances than either alone, but this is rarely used commercially because technical difficulties in maintaining mixed cultures. Typical problems with cultures on scale are unrestrained growth one bacterium, which leads to suboptimal population ratios lack control over spatial distribution, inefficient substrate transport. To address these issues, we designed produced a synthetic...
Enzymes of natural biochemical pathways are routinely subcellularly organized in space and time order to improve pathway efficacy control. Designer scaffolding platforms under development confer similar benefits upon engineered pathways. Herein, we evaluate bacterial microcompartment shell (pfam0936-domain) proteins as modules for constructing well-defined nanometer scale scaffolds vivo. We use a suite visualization techniques scaffold assembly dynamics. demonstrate recruitment target cargo...
Carboxysomes are a class of bacterial microcompartments that form proteinaceous organelles within the cytoplasm cyanobacteria and play central role in photosynthetic metabolism by defining cellular microenvironment permissive to CO2 fixation. Critical aspects assembly carboxysomes remain relatively unknown, especially with regard dynamics this microcompartment. Progress understanding carboxysome is impeded part because analysis subtle changes morphology microscopy remains low-throughput...
Microbial communities have vital roles in systems essential to human health and agriculture, such as gut soil microbiomes, there is growing interest engineering designer consortia for applications biotechnology ( e . g ., personalized probiotics, bioproduction of high-value products, biosensing). The capacity monitor model metabolite exchange dynamic microbial can provide foundational information important understand the community level behaviors that emerge, a requirement building novel...
Surface display technologies have been primarily developed for heterotrophic microbes, leaving photosynthetic counterparts like cyanobacteria with limited molecular tools. Here, we expanded upon surface systems in
Abstract Microbial communities have vital roles in systems essential to human health and agriculture, such as gut soil microbiomes, there is growing interest engineering designer consortia for applications biotechnology ( e.g ., personalized probiotics, bioproduction of high-value products, biosensing). The capacity monitor model metabolite exchange dynamic microbial can provide foundational information important understand the community level behaviors that emerge, a requirement building...
Bioencapsulation is a low-cost, low energy demand technology for harnessing the power of microorganisms applications such as bioremediation, biosensing, and biocatalysis. Silica gels have been investigated bioencapsulation, yet few studies focused on gel mechanical properties biocatalytic activity simultaneously, instead studying viability encapsulated microorganisms. In order to utilize these unique functionalities in context industrially relevant processes, bioencapsulation materials with...
Abstract Photosynthetic organisms possess a variety of mechanisms to achieve balance between absorbed light (source) and the capacity metabolically utilize or dissipate this energy (sink). While regulatory processes that detect changes in metabolic status/balance are relatively well-studied plants, analogous pathways remain poorly characterized photosynthetic microbes. Herein, we explore systemic result from alterations carbon availability model cyanobacterium Synechococcus elongatus PCC...
Abstract A computational framework combining Agent-Based Models (ABMs) and Deep Learning techniques was developed to help design microbial communities that convert light CO 2 into useful bioproducts. An ABM accounts for , light, sucrose export rate cell-to-cell mechanical interactions used investigate the growth of an engineered sucrose-exporting strain Synechococcus elongatus PCC 7942. The simulations produced population curves synthetic images colony growth. were analyzed, correlated...
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1. Abstract There has been substantial recent interest in the promise of sustainable, light-driven bioproduction using cyanobacteria, including developing efforts for microbial mixed autotroph/heterotroph communities, which could provide useful properties, such as division metabolic labor. However, building stable mixed-species communities sufficient productivity remains a challenge, partly due to lack strategies synchronizing and coordinating biological activities across different species....
Abstract Carboxysomes are a class of bacterial microcompartments that form proteinaceous organelles within the cytoplasm cyanobacteria and play central role in photosynthetic metabolism by defining cellular microenvironment permissive to CO 2 fixation. Critical aspects assembly carboxysomes remain relatively unknown, especially with regard dynamics this microcompartment. We have recently expressed an exogenous protease as way gaining control over endogenous protein levels, including...