A5007531872- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Phytase and its Applications
- Electrochemical sensors and biosensors
- Microbial Applications in Construction Materials
- Enzyme Structure and Function
- Protein Hydrolysis and Bioactive Peptides
- Proteins in Food Systems
- Microbial Metabolites in Food Biotechnology
- Graphene and Nanomaterials Applications
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Biochemical Acid Research Studies
- Protein Interaction Studies and Fluorescence Analysis
- Biochemical and Molecular Research
- Food composition and properties
- Algal biology and biofuel production
- Cassava research and cyanide
- Electrochemical Analysis and Applications
- Metabolism and Genetic Disorders
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- Carbon and Quantum Dots Applications
- Natural Antidiabetic Agents Studies
- Nanoparticles: synthesis and applications
Banaras Hindu University
2015-2024
Ruhr University Bochum
2005
National Institute of Diabetes and Digestive and Kidney Diseases
1991-1994
National Institutes of Health
1991-1994
Shimane University
1991
Kōchi University
1991
Physico-chemical properties reflect the functional and structural characteristics of a protein. The comparative study physicochemical is important to know role protein in exploring its molecular evolution. A number online offline tools are available for calculating physico-chemical single sequence. However, tool not with graphical visualization Multi-FASTA sequences. Hence, we describe development utility MFPPI V.1.0 (a web interface developed JAVA platform) input each FASTA sequence from...
In this case study, we analysed the properties of unfolded states and pathways leading to complete denaturation a multimeric chick pea β-galactosidase (CpGAL), as obtained from treatment with guanidium hydrochloride, urea, elevated temperature extreme pH.CpGAL, heterodimeric protein native molecular mass 85 kDa, belongs α+β class protein. The conformational stability thermodynamic parameters CpGAL unfolding in different were estimated interpreted using circular dichroism fluorescence...
Background β-Galactosidase is a vital enzyme with diverse application in molecular biology and industries. It was covalently attached onto functionalized graphene nano-sheets for various analytical applications based on lactose reduction. Methodology/Principal Findings Response surface methodology Box-Behnken design of experiment used determination optimal immobilization conditions, which resulted 84.2% efficiency. Native immobilized characterized the help transmission scanning electron...
Urease purified from pigeonpea seeds was immobilized on gelatin beads via cross‐linking with glutaraldehyde. The maximum immobilization (75%) observed at 30 mg/ml gelatin, 0·414 mg of enzyme/bead, 1% (v/v) glutaraldehyde and 4 °C. Beads stored in 50 mM Tris/acetate buffer (pH 7·3) °C showed a half‐life 240 days there practically no leaching enzyme (less than 2%) over period days. These can be reused more times (with 24 h intervals) without much loss activity (i.e. less 11%). urease shift its...
The origin of reaction and substrate specificity the control activity by protein-protein interaction are investigated using tryptophan synthase alpha 2 beta complex from Salmonella typhimurium. We have compared some spectroscopic kinetic properties wild type subunit five mutant forms that altered catalytic properties. These enzymes, which were engineered site-directed mutagenesis, single amino acid replacements in either active site or wall a tunnel extends to complex. find complexes...