A5091032996- Electrochemical Analysis and Applications
- Mass Spectrometry Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Trace Elements in Health
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Advanced ceramic materials synthesis
- Analytical Chemistry and Sensors
- Various Chemistry Research Topics
- Animal Virus Infections Studies
- Ion-surface interactions and analysis
- Biosensors and Analytical Detection
- Heavy Metal Exposure and Toxicity
- Analytical Chemistry and Chromatography
University of Manchester
2024-2025
Adeno-associated viruses (AAVs) are at the forefront of biopharmaceutical development as gene therapy vectors. The successful approval these medicines requires robust characterization including an assessment therapeutic content. Using ion mobility–mass spectrometry (IM-MS) techniques, we determine empty:full capsid ratios and explore structural differences in AAV particles relevant to payload. With drift tube IM-MS, demonstrate that empty capsids, while slightly smaller, present more...
Ion mobility-mass spectrometry (IM-MS) unraveled different conformational stability in Zn4-7-metallothionein-2. We introduced a new molecular dynamics simulation approach that permitted the exploration of all space confirming experimental data, and revealed not only Zn-S bonds but also α-β domain interactions modulate protein unfolding.
ABSTRACT The fundamental biology of Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (Ncap), its use in diagnostic assays and potential application as a vaccine component have received considerable attention since the outbreak Covid19 pandemic late 2019. Here we report scalable expression purification soluble, immunologically active, SARS-CoV-2 Ncap Escherichia coli . Codon-optimised synthetic genes encoding original sequence four common variants with an...
The fundamental biology of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (Ncap), its use in diagnostic assays and potential application as a vaccine component have received considerable attention since the outbreak Covid19 pandemic late 2019. Here we report scalable expression purification soluble, immunologically active, SARS-CoV-2 Ncap Escherichia coli. Codon-optimised synthetic genes encoding original sequence four common variants with an N-terminal...
Mammalian zinc metallothionein-3 (Zn7MT3) plays an important role in protecting against copper toxicity by scavenging free Cu(II) ions or removing bound to β-amyloid and α-synuclein. While previous studies reported that Zn7MT3 reacts with form Cu(I)4Zn(II)4MT3ox containing two disulfides (ox), the precise localization of metal remained unclear. Here, we undertook comprehensive structural characterization metal-protein complexes formed reaction between using native ion mobility mass...
During the COVID-19 pandemic, development of diagnostic tests was vital to chart course and reduce impact infection. Continued testing surveillance vaccine escape will continue for years come which presents an opportunity integrate such into clinical biochemistry laboratories that form part integrated healthcare testing. Here we describe a protocol targeted mass spectrometry based proteomic assay (COVIDCAP) developed detect SARS-CoV-2 peptides from oro-nasopharyngeal swabs (ONP) saliva. This...
A lab-based experiment, combining ion mobility mass spectrometry (IM-MS) with computational modelling techniques for the analysis of small molecules, has been developed use in undergraduate teaching laboratories over eight years. Students link IM-MS calculations to show how their interplay can elucidate structures molecules chosen by students. This experiment offers an opportunity gain experience a wide range skills including experimental design, sample preparation, instrument tuning,...
Ion mobility-mass spectrometry (IM-MS) unraveled different conformational stability in Zn 4-7 -metallothionein-2. We introduced a new molecular dynamics simulations approach that permitted the exploration of all space confirming experimental data, and revealed not only Zn–S bonds but also α–β domain interaction modulates protein unfolding.