A5028270504- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Lipid metabolism and biosynthesis
- Advanced Proteomics Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Acute Myeloid Leukemia Research
- Microtubule and mitosis dynamics
- Analytical chemistry methods development
- Advanced biosensing and bioanalysis techniques
- Plant responses to water stress
- Cancer Treatment and Pharmacology
- Immune responses and vaccinations
- Autophagy in Disease and Therapy
- Sinusitis and nasal conditions
- Multiple Myeloma Research and Treatments
- Ion-surface interactions and analysis
- Pesticide Residue Analysis and Safety
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- interferon and immune responses
- Respiratory viral infections research
- Cellular transport and secretion
- Burkholderia infections and melioidosis
- Peptidase Inhibition and Analysis
- SARS-CoV-2 detection and testing
Newcastle University
2021-2024
The Kids Research Institute Australia
2024
The University of Western Australia
2024
Iowa State University
2016-2023
University of California, Los Angeles
2022
Ames National Laboratory
2016-2018
East Carolina University
2013-2014
Universidad San Francisco de Quito
1969
Abstract The nasal epithelium is a plausible entry point for SARS-CoV-2, site of pathogenesis and transmission, may initiate the host response to SARS-CoV-2. Antiviral interferon (IFN) responses are critical outcome Yet little known about interaction between SARS-CoV-2 innate immunity in this tissue. Here we apply single-cell RNA sequencing proteomics primary cell model human differentiated at air-liquid interface. demonstrates widespread tropism epithelial types. dominated by type I III...
Abstract The zebrafish ( Danio rerio ) has been widely used as a model vertebrate system to study lipid metabolism, the roles of lipids in diseases, and dynamics embryonic development. Here, we applied high-spatial resolution matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) map visualize three-dimensional spatial distribution phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamines (PE), phosphatidylinositol (PI), newly fertilized...
Exploring the metabolic differences directly on tissues is essential for comprehensive understanding of how multicellular organisms function. Mass spectrometry imaging (MSI) an attractive technique toward this goal; however, MSI in metabolomics scale has been hindered by multiple limitations. This most notable single cell level high-spatial resolution because limited number molecules small sampling size and low ionization yields many metabolites. Several on-tissue chemical derivatization...
Thermal proteome profiling (TPP) provides a powerful approach to studying proteome-wide interactions of small therapeutic molecules and their target off-target proteins, complementing phenotypic-based drug screens. Detecting differences in thermal stability due engagement requires high quantitative accuracy consistent detection. Isobaric tandem mass tags (TMTs) are used multiplex samples increase quantification precision TPP analysis by data-dependent acquisition (DDA). However, advances...
High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at cellular and subcellular level. In this work, we improved our previous laser optical system matrix-assisted desorption ionization (MALDI)-MSI, from ~9 μm practical spot size to a ~4 μm, thereby allowing 5 without oversampling. This accomplished through combination spatial filtering, beam expansion, reduction final focal length. Most importantly, new optics allows simple...
Metabolism in plants is compartmentalized among different tissues, cells and subcellular organelles. Mass spectrometry imaging (MSI) with matrix-assisted laser desorption ionization (MALDI) has recently advanced to allow for the visualization of metabolites at single-cell resolution. Here we applied 5- 10 μm high spatial resolution MALDI-MSI asymmetric Kranz anatomy Zea mays (maize) leaves study differential localization two major anionic lipids thylakoid membranes,...
Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos leaves. A systematic study was performed varying solvent incubation time. During this study, unexpected side reactions were found when methanol used as a solvent, resulting the formation of methyl ester phosphatidic acid. Using an optimum condition with isopropanol, there no apparent delocalization demonstrated transmission electron microscopy (TEM) pattern leaf images obtained at 10 μm spatial...
Nanoparticles are efficient matrices in laser desorption/ionization (LDI) mass spectrometry (MS), especially for the profiling or imaging of small molecules. Recently, solvent-free physical vapor desorption (PVD), sputter coating, was adopted as a homogenous method to rapidly apply metal nanoparticles (NPs) situ samples prior LDI MS analysis. However, there has been no systematic study comparing different targets analysis variety molecule metabolites. Here, we present screening and...
Abstract Derivatization reactions are commonly used in mass spectrometry to improve analyte signals, specifically by enhancing the ionization efficiency of those compounds. Vicinal diols one group biologically important compounds that have been derivatized using boronic acid. In this study, a acid with tertiary amine was adapted for derivatization vicinal diol metabolites B73 maize tissue cross‐sections imaging analysis. Using method, dozens were derivatized, effectively improving signal...
Autophagy is a conserved mechanism among eukaryotes that degrades and recycles cytoplasmic components. known to influence the plant metabolome, including lipid content; however, its impact on lipidome not fully understood, most studies have analyzed single or few mutants defective in autophagy. To gain more insight into effect of autophagy concentrations composition, we quantitatively profiled glycerolipids from multiple Arabidopsis thaliana altered compared them with wild-type seedlings...
Inflammatory responses are important in cancer, particularly the context of monocyte-rich aggressive myeloid neoplasm. We developed a label-free cellular phenotypic drug discovery assay to identify anti-inflammatory drugs human monocytes derived from acute leukemia (AML), by tracking several features ionizing only 2500 cells using matrix-assisted laser desorption/ionization-time flight (MALDI-TOF) mass spectrometry. A proof-of-concept screen showed that BCR-ABL inhibitor nilotinib, but not...
MALDI-TOF MS is a powerful analytical technique that provides fast and label-free readout for in vitro assays the high-throughput screening (HTS) environment. Here, we describe development of novel, HTS compatible, MS-based drug discovery assay endoplasmic reticulum aminopeptidase 1 (ERAP1), an important target immuno-oncology auto-immune diseases. A was developed beginning with already established ERAP1 RapidFire (RF MS) assay, where peptide YTAFTIPSI trimmed into product TAFTIPSI. We noted...
During the COVID-19 pandemic, development of diagnostic tests was vital to chart course and reduce impact infection. Continued testing surveillance vaccine escape will continue for years come which presents an opportunity integrate such into clinical biochemistry laboratories that form part integrated healthcare testing. Here we describe a protocol targeted mass spectrometry based proteomic assay (COVIDCAP) developed detect SARS-CoV-2 peptides from oro-nasopharyngeal swabs (ONP) saliva. This...
Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study function. Currently, there few tools the spatial dependence of consequences silencing at cellular level.We report use multimodal Raman and mass spectrometry imaging cellular-level biochemical changes that occur from phytoene desaturase (pds) using a Foxtail mosaic virus (FoMV) vector in maize leaves. The method allows localized carotenoid distribution be measured reveals differences lost...
Abstract Thermal proteome profiling (TPP) provides a powerful approach to studying proteome-wide interactions of small therapeutic molecules and their target off-target proteins, complementing phenotypic-based drug screens. Detecting differences in thermal stability due engagement requires high quantitative accuracy consistent detection. Isobaric tandem mass tags (TMT) are used multiplex samples increase quantification precision TPP analysis by data-dependent acquisition (DDA). However,...