A5087334841- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Plant biochemistry and biosynthesis
- Research in Cotton Cultivation
- Mass Spectrometry Techniques and Applications
- Cancer therapeutics and mechanisms
- DNA Repair Mechanisms
- Chronic Lymphocytic Leukemia Research
- Analytical Chemistry and Chromatography
- Lymphoma Diagnosis and Treatment
- Analytical chemistry methods development
- Plant responses to water stress
- Botanical Research and Chemistry
- Microbial Metabolic Engineering and Bioproduction
- Plant Surface Properties and Treatments
- Organ Transplantation Techniques and Outcomes
- CAR-T cell therapy research
- Viral-associated cancers and disorders
- Plant Molecular Biology Research
- Aquaculture Nutrition and Growth
- Algal biology and biofuel production
- Rice Cultivation and Yield Improvement
- Acute Lymphoblastic Leukemia research
- Protein Degradation and Inhibitors
- Advanced Proteomics Techniques and Applications
The University of Texas Southwestern Medical Center
2020-2023
University of North Texas
2013-2021
Plant (United States)
2020
Huazhong Agricultural University
2020
Summary Despite the importance of oilseeds to worldwide human nutrition, and more recently production bio‐based diesel fuels, detailed mechanisms regulating seed oil biosynthesis remain only partly understood, especially from a tissue‐specific perspective. Here, we investigated spatial distributions lipid metabolites transcripts involved in seeds two low‐erucic acid genotypes Brassica napus with high low seed‐oil content. Integrated results matrix‐assisted laser desorption/ionization‐mass...
Seeds of the desert shrub, jojoba (Simmondsia chinensis), are an abundant, renewable source liquid wax esters, which valued additives in cosmetic products and industrial lubricants. Jojoba is relegated to its own taxonomic family, there little genetic information available elucidate phylogeny. Here, we report high-quality, 887-Mb genome assembled into 26 chromosomes with 23,490 protein-coding genes. The has only whole-genome triplication (γ) shared among eudicots no recent duplications....
There is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic (DHA), not least all given importance these both aquaculture human nutrition. Previously we have demonstrated feasibility using metabolic engineering transgenic plants (Camelina sativa) to modify seed oil composition now include EPA and/or DHA. In this study, further tailored profile reduce omega-6 content, evaluated performance GM...
The regulation of lipid synthesis in oil seeds is still not fully understood. Oilseed rape (Brassica napus) the third most productive vegetable crop on global market; therefore, increasing our understanding accumulation oilseed great economic, as well intellectual, importance. Matrix-assisted laser/desorption ionization-mass spectrometry imaging (MALDI-MSI) a technique that allows mapping metabolites directly onto intact biological tissues, giving spatial context to metabolism. We have used...
Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, of great importance for seed oil quality. The effects increased or decreased expression ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE (PDAT) on lipid composition were assessed in several Camelina sativa lines. Furthermore, vitro assays acyltransferases microsomal fractions prepared from developing seeds some these lines performed. Decreased DGAT1 led an...
A simple and rapid digestion method is reported for the simultaneous acid extraction of chromium, copper, zinc, cadmium, nickel lead from high-fat fish tissue. Samples are digested with nitric sulphuric acids at 150 °C in a modified aluminium hot-block. The specially set up sample sizes to 5 g, low level detection these elements. After digestion, extracts analysed by direct flame atomic-absorption spectrometry zinc chromium. other three elements, lead, concentrated chelation ammonium...
Fat storage-inducing transmembrane protein 2 (FIT2) is an endoplasmic reticulum (ER)-localized that plays important role in lipid droplet (LD) formation animal cells. However, no obvious homologue of FIT2 found plants. Here, we tested the function plant cells by ectopically expressing mouse (Mus musculus) Nicotiana tabacum suspension-cultured cells, benthamiana leaves and Arabidopsis thaliana Confocal microscopy indicated expression dramatically increased number size LDs N. Arabidopsis,...
Urushiols are the allergenic components of Toxicodendron radicans (poison ivy) as well other species. They alk-(en)-yl catechol derivatives with a 15- or 17-carbon side chain having different degrees unsaturation. Although several methods have been developed for analysis urushiols in plant tissues, situ localization urushiol congeners has not reported. Here, we report on first poison ivy stems by matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). Our results...
We describe a novel technique combining precise organelle microextraction with deposition and matrix-assisted laser desorption/ionization (MALDI) for rapid, minimally invasive mass spectrometry (MS) analysis of single organelles from living cells. A dual-positioner nanomanipulator workstation was utilized both extraction content co-deposition analyte matrix solution MALDI-direct (DOMS) analysis. Here, the triacylglycerol (TAG) profiles lipid droplets 3T3-L1 adipocytes were acquired results...
Historically, the primary objective of cotton ( Gossypium hirsutum L.) breeding programs was to improve quantity and quality fiber. Because added value cottonseed its many uses, including a feed human food source, there is interest in developing that focus improvement efforts simultaneously on fiber seed. Genetic analysis traits, such as protein oil, prerequisite building new joint seed programs. In this study, we conducted genetic diverse set elite upland germplasm for oil. Environment...
A new single Peltier element ablation cell is described and its applicability to biological sampling discussed evaluate performance.
Brassica napus (B. napus) is the world's most widely grown temperate oilseed crop. Although breeding for human consumption has led to removal of erucic acid from refined canola oils, there renewed interest in industrial uses derived B. napus, and a rich germplasm available use. Here, low- high-erucic accessions seeds were examined distribution metabolites gene transcripts encoding enzymatic pathways its incorporation into triacylglycerols (TAGs) across major tissues seeds. In general,...
Rationale Refined cottonseed oil has widespread applications in the food and chemical industries. Although major lipids comprising (triacylglycerols) are well known, there many diverse lipid species cotton seeds that occur at much lower levels have important nutritional or anti‐nutritional properties. Methods The technical samples were prepared chloroform. biological extracted using a mixture of isopropanol/chloroform/H 2 O (2:1:0.45). data collected high low collision energy with...
Over the last decade next-generation sequencing and ‘omics techniques have become indispensable tools for medicine drug discovery. These led to an explosion of publicly available data that often goes under-utilized due lack bioinformatic expertise analyze volume data. Here, we demonstrate power applying two novel computational platforms, NCI’s CellMiner Cross Database Lantern Pharma’s proprietary artificial intelligence (AI) machine learning (ML) RADR ® platform, identify biological insights...