A5062599726- Effects of Radiation Exposure
- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Radiation Therapy and Dosimetry
- Synthetic Organic Chemistry Methods
- Carcinogens and Genotoxicity Assessment
- Biochemical and Molecular Research
- DNA Repair Mechanisms
- Lymphoma Diagnosis and Treatment
- Enzyme Structure and Function
- Radiation Dose and Imaging
- Chemical Synthesis and Analysis
- Lipid Membrane Structure and Behavior
- Cancer, Hypoxia, and Metabolism
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Genetic factors in colorectal cancer
- Analytical Chemistry and Chromatography
- Microbial Metabolic Engineering and Bioproduction
- Cancer Treatment and Pharmacology
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Myasthenia Gravis and Thymoma
National Institutes for Quantum Science and Technology
2014-2024
Institute for Environmental Sciences
2024
Radiation Effects Research Foundation
2018-2024
Tokyo Metropolitan University
2021-2022
Ibaraki University
2017-2019
Tokyo Institute of Technology
1998-2018
National Institute of Health Sciences
2014
Institute for Biotechnology and Bioengineering
2012
In-Q-Tel
2012
Hokkaido University
2011
Abstract We investigated a role of chemokines in thymocyte trafficking. Genes encoding stromal cell-derived factor-1 and its receptor CXCR4 were detected the cortex by situ hybridization. Early immigrant cells did not express CXCR4, whereas their descendant CD44+CD25+CD4−CD8− did. expression was down-modulated when CD4+CD8+ double-positive became CD4+CD8− or CD4−CD8+ single-positive (SP) cells. Positively selected CD69+CD3intermediate gained CCR4, which ligand, thymus activation-regulated...
Seven isolates of feline immunodeficiency virus (FIV), Shizuoka, Yokohama, Sendai-1, Sendai-2, Fukuoka, Aomori-1, and Aomori-2, were isolated from FIV-seropositive domestic cats in Japan, their proviral DNAs amplified by PCR. The nucleotide sequences env gag genes determined compared with those previously described isolates: U.S. European one Japanese isolate, TM2. Phylogenetic analyses complete gene demonstrate that worldwide are classified into three subtypes: TM2, non-Japanese subtypes...
We have previously reported the DNA and amino acid sequences for three genes (ntpA, ntpB, ntpK) encoding A, B, K (proteolipid) subunits, respectively, of Na+-translocating ATPase a eubacterium Enterococcus hirae (
Four non-adjacent bis-tetrahydrofuranic acetogenins, named squamostatins-B (2), -C (3), -D (4) and -E (5), have been isolated from the petroleum ether extract of Annona squamosa seeds. The structures these acetogenins established on basis spectral evidence. C-15/C-16-threo, C-19/C-20-threo, C-23/C-24-erythro stereochemistry was assigned for -D, whereas C-23/C-24-threo assigened squamostatins-C -E. All including squamostatin-A, to C-12/C-15-trans, C-20/C-23-trans by 13C-NMR comparison with...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe structure of asukamycin, a possible shunt metabolite from 3-dehydroquinic acid in the shikimate pathwayKatsumi Kakinuma, Nobuo Ikekawa, Akira Nakagawa, and Satoshi OmuraCite this: J. Am. Chem. Soc. 1979, 101, 12, 3402–3404Publication Date (Print):June 1, 1979Publication History Published online1 May 2002Published inissue 1 June 1979https://pubs.acs.org/doi/10.1021/ja00506a056https://doi.org/10.1021/ja00506a056research-articleACS...
The petroleum ether extract of the seeds Annona squamosa L. yielded thirteen adjacent and four non-adjacent bis-tetrahydrofuranic acetogenins, in addition to squamocin (1) squamostatin-A. structures named as squamocins-B (2), -C (3), -D (4), -E (5), -F (6), -G (7), -H (8), -I (9), -J (10), -K (11), -L(12), -M (13) -N (14), have been established on basis spectral evidence. Squamocins-B, -D, -E, -F, -I, are new acetogenins. these acetogenins vary hte carbon number (C37 or C35), position...
Threo-Ds-3-isopropylmalate dehydrogenase coded by the leuB gene from an extreme thermophile, Thermus thermophilus strain HB8, was expressed in Escherichia coli carrying a recombinant plasmid. The thermostable enzyme thus produced extracted E. cells, purified, and crystallized. shown to be dimer of identical subunits molecular weight (4.0±0.5)×l04 Km for threo-Ds-3-isopropyl-malate estimated 8.0×10−5 M that NAD 6.3×10−4 M. optimum pH at 75°C presence 1.2 KCl around 7.2. Mg2+ or Mn2+ essential...
Squamocin, a new trihydroxy-bis-tetrahydrofuran fatty acid γ-lactone (acetogenin), has been isolated from Annona squamosa L.(Annonaceae) and its structure elucidated on the basis of spectral evidence chemical degradations.
Bacteria show remarkable adaptability under several stressful conditions by shifting themselves into a dormant state. Less is known, however, about the mechanism underlying cell transition to dormancy. Here, we report that states mediated one of major toxin-antitoxin systems, RelEB, in density-dependent manner Escherichia coli K-12 MG1655. We constructed strain, IKA121, which expresses toxin RelE presence rhamnose and lacks chromosomal relBE rhaBAD. With this demonstrated RelE-mediated...
HLE, a human hepatocellular carcinoma cell line was transiently transfected with normal MnSOD and without mitochondrial targeting signal (MTS). Mitochondrial reactive oxygen species (ROS), lipid peroxidation apoptosis were examined as function of time following 18.8 Gy X-ray irradiation. Our results showed that the level ROS increased reached maximum 2 hours after Authentic MnSOD, but not lacking MTS, protected against ROS, apoptosis. In addition, levels consistently found to always...
Total synthesis of archaeal 36-membered macrocyclic diether lipid 2 is reported. The based upon stereoselective preparation functionalized isoprenoid chains, ether-linkage formation between the chains with a glycerol derivative, and ultimate intramolecular dicarbonyl coupling using low-valent titanium known as McMurry coupling. This synthetic method has successfully provided first practical route to chemically defined membrane lipids, which were not available because lack access. Also...
The cAMP-dependent protein kinase of the cellular slime mold, Dictyostelium discoideum, is developmentally regulated; there an approximately 4-fold increase in activity during development. incorporation [3H]leucine into enzyme demonstrates that de novo synthesis kinase. activities catalytic and regulatory subunits parallel. maximal rate precedes tip formation, a stage development characterized by sharp mRNA complexity. high level activity, attained at this development, persists when...
Glycosyltransferase VinC was explored for a construction of glycoside libraries using dTDP-vicenisamine and structurally unrelated unnatural aglycons, new vicenisaminides were successfully constructed. Structural elements aglycon recognition by proposed modeling studies confirmed the success transglycosylation upon designed aglycon.