A5010203311- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Chemical Synthesis and Analysis
- Angiogenesis and VEGF in Cancer
- Gene Regulatory Network Analysis
- Venous Thromboembolism Diagnosis and Management
- Cardiac and Coronary Surgery Techniques
- RNA Research and Splicing
- Atrial Fibrillation Management and Outcomes
- Hematopoietic Stem Cell Transplantation
- RNA modifications and cancer
- Maternal Mental Health During Pregnancy and Postpartum
- Peptidase Inhibition and Analysis
- Mesenchymal stem cell research
- Treatment of Major Depression
- Circadian rhythm and melatonin
- Genetics, Bioinformatics, and Biomedical Research
- vaccines and immunoinformatics approaches
- Cancer Cells and Metastasis
- Misinformation and Its Impacts
- Cancer, Hypoxia, and Metabolism
- Microbial Natural Products and Biosynthesis
- Pluripotent Stem Cells Research
- Pancreatic function and diabetes
- Family Caregiving in Mental Illness
Synthetic Biologics (United States)
2022
Massachusetts Institute of Technology
2018-2022
Portland State University
2016
Concord Hospital
2013
Vanderbilt University Medical Center
2009
Vanderbilt University
2007
Oregon Health & Science University
2004-2005
VA Sepulveda Ambulatory Care Center
1994
Neuropsychiatric Research Institute
1994
San Francisco General Hospital
1994
Despite an important role in vascular development and repair, the origin of endothelial progenitors remains unknown. Accumulating evidence indicates that cells derived from hematopoietic system participate angiogenesis. However, identity functional these remain controversial. Here we show can differentiate common myeloid granulocyte/macrophage progenitors. Endothelial transplanted bone marrow-derived lineage expressed CD31, von Willebrand factor, Tie2 but did not express markers CD45 F4/80...
Abstract Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity units RNAP/s can be inferred. This work facilitates reporting promoters absolute units, variability their across population, quantitative toll on cellular resources, all which provide critical insights engineering.
Recent evidence indicates that bone marrow is a source of endothelial progenitor cells are mobilized into the peripheral blood in response to cytokines or tissue injury. Previously, we showed functional (ECs) can be clonally derived from phenotypically defined hematopoietic stem cells. To determine EC potential human and cells, vessels sex-mismatched transplant recipients were evaluated. outcomes identified by using combination immunohistochemistry XY interphase FISH. Donor-derived ECs...
RNA-binding proteins (RBPs) play critical roles in regulating gene expression by modulating splicing, RNA stability, and protein translation. Stimulus-induced alterations RBP function contribute to global changes expression, but identifying which RBPs are responsible for the observed remains an unmet need. Here, we present Transite, a computational approach that systematically infers influencing through stability degradation. As proof of principle, apply Transite data from human patients...
Peptide secondary metabolites are common in nature and have diverse pharmacologically-relevant functions, from antibiotics to cross-kingdom signaling. Here, we present a method design large libraries of modified peptides Escherichia coli screen them vivo identify those that bind single target-of-interest. Constrained peptide scaffolds were produced using enzymes gleaned microbial RiPP (ribosomally synthesized post-translationally peptide) pathways diversified build libraries. The binding...
Article8 November 2021Open Access Transparent process Competitive dCas9 binding as a mechanism for transcriptional control Daniel A Anderson orcid.org/0000-0001-9655-323X Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute Technology, Cambridge, MA, USA Search more papers by this author Christopher Voigt Corresponding Author [email protected] orcid.org/0000-0003-0844-4776 Information Anderson1 and *,1 1Synthetic *Corresponding author. Tel: +1 617 324 4851;...
RiPPs (ribosomally-synthesized and post-translationally modified peptides) are a class of pharmaceutically-relevant natural products expressed as precursor peptides before being enzymatically processed into their final functional forms. Bioinformatic methods have illuminated hundreds thousands RiPP enzymes in sequence databases the number characterized chemical modifications is growing rapidly; however, it remains difficult to functionally express them heterologous host. One challenge...
Synthetic biology requires students and scientists to draw upon knowledge expertise from many disciplines. While this diversity is one of the field's primary strengths, it also makes challenging for newcomers acquire background necessary thrive. To address gap, we developed a course that provides structured approach learning biological principles theoretical underpinnings synthetic biology. Our course, Principles Biology (PoSB), was released on massively open online platform edX in 2016....
Transgenic marking approaches are increasingly used to evaluate the developmental potential of stem cells. However, cell fate mapping studies using different transgenic systems have produced conflicting results. These disparate findings may be due in part infidelity donor marker gene expression. Analysis hematopoietic cells (c-Kit+, Sca-1+, lineage marker- [KSL]) from a mouse (1Osb) engineered ubiquitously express enhanced green fluorescent protein (EGFP) reveals two distinct populations....
In October 2009, the U.S. Pharmacopoeia (USP) changed monograph for heparin to bring USP units in line with international heparin. The result was a 10% decrease potency as measured by vitro laboratory tests. This led questions regarding dosing guidelines. There existed need an vivo study determine practical changes that may be implemented regard administration cardiopulmonary bypass clinical setting. A retrospective conducted dose administered and corresponding effect on patients undergoing...
Abstract RNA-binding proteins (RBPs) play critical roles in regulating gene expression by modulating splicing, RNA stability, and protein translation. In response to various stimuli, alterations RBP function contribute global changes expression, but identifying which specific RBPs are responsible for the observed patterns remains an unmet need. Here, we present Transite a multi-pronged computational approach that systematically infers influencing through stability degradation. As proof of...