A5015616665- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Force Microscopy Techniques and Applications
- Protein Kinase Regulation and GTPase Signaling
- Advanced Memory and Neural Computing
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Geriatric Care and Nursing Homes
- Animal Genetics and Reproduction
- Supramolecular Self-Assembly in Materials
- Retinal Development and Disorders
- Cell Adhesion Molecules Research
- Digitalization, Law, and Regulation
- Mitochondrial Function and Pathology
- Interprofessional Education and Collaboration
- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- Mathematical Biology Tumor Growth
University of California, San Diego
2017-2024
Jacobs (United States)
2021-2024
University of California System
2019
Dendritic spines are small subcompartments that protrude from the dendrites of neurons and important for signaling activity synaptic communication. These have been characterized to different shapes. While it is known these shapes associated with spine function, specific nature shape-function relationships not well understood. In this work, we systematically investigated relationship between shape size both head apparatus, a specialized endoplasmic reticulum compartment within head, in...
Abstract Axons are ultrathin membrane cables that specialized for the conduction of action potentials. Although their diameter is variable along length, how morphology determined unclear. Here, we demonstrate unmyelinated axons mouse central nervous system have nonsynaptic, nanoscopic varicosities ~200 nm in repeatedly length interspersed with a thin cable ~60 like pearls-on-a-string. In silico modeling suggests this axon nanopearling can be explained by mechanical properties. Treatments...
Dendritic spines are small, bulbous protrusions along the dendrites of neurons and sites excitatory postsynaptic activity. The morphology has been implicated in their function synaptic plasticity shapes have well-characterized, but potential mechanics underlying shape development maintenance not yet fully understood. In this work, we explore mechanical principles that could underlie specific using a minimal biophysical model membrane-actin interactions. Using model, first identify possible...
Dendritic spines act as biochemical computational units and must adapt their responses according to activation history. Calcium influx acts the first signaling step during postsynaptic is a determinant of synaptic weight change. also come in variety sizes shapes. To probe relationship between calcium dynamics spine morphology, we used stochastic reaction-diffusion model idealized realistic geometries. We show that despite nature various channels, receptors, pumps, size shape can modulate...
Abstract The shape of the cell is connected to its function; however, we do not fully understand underlying mechanisms by which global regulates a cell’s functional capabilities. Using theory, experiments and simulation, investigated how physiologically relevant changes affect subcellular organization, consequently intracellular signaling, control information flow needed for phenotypic function. Vascular smooth muscle cells going from proliferative motile circular contractile fusiform show...
The endoplasmic reticulum (ER) is a tortuous organelle that spans throughout cell with continuous membrane containing ion channels, pumps, and transporters. It unclear if stimuli gate ER channels trigger substantial potential fluctuations those spread beyond their site of origin. Here, we visualize dynamics in HEK cells cultured rat hippocampal neurons by targeting genetically encoded voltage indicator specifically to the membrane. We report existence clear cell-type- stimulus-specific...
Abstract Axons are thought to be ultrathin membrane cables of a relatively uniform diameter, designed conduct electrical signals, or action potentials. Here, we demonstrate that unmyelinated axons not simple cylindrical tubes. Rather, have nanoscopic boutons repeatedly along their length interspersed with thin cable diameter ∼60 nm like pearls-on-a-string. These only ∼200 in and do synaptic contacts cluster vesicles, hence non-synaptic. Our silico modeling suggests axon pearling can...
The formation and disruption of synaptic connections during development are a fundamental step in neural circuit formation. Subneuronal structures such as neurites known to be sensitive the level spontaneous neuronal activity, but specifics how neurotransmitter-induced calcium activity regulates neurite homeostasis not yet fully understood. In response stimulation by neurotransmitters acetylcholine, responses cells mediated Gαq/phospholipase Cβ (PLCβ)/phosphatidylinositol 4,5-bisphosphate (PI(4,5)P
Synaptic plasticity involves modification of both biochemical and structural components neurons. Many studies have revealed that the change in number density glutamatergic receptor AMPAR at synapse is proportional to synaptic weight update; an increase corresponds strengthening synapses while a decrease weakens connections. The dynamics are thought be regulated by upstream signalling, primarily calcium-CaMKII pathway, trafficking from synapse, influx extrasynaptic sources. Previous work...
ABSTRACT The shape of the cell is connected to its function; however, we do not fully understand underlying mechanisms by which global regulates a cell’s functional capabilities. Using theory, experiments and simulation, investigated how physiologically relevant changes affect subcellular organization, consequently intracellular signaling, control information flow needed for phenotypic function. Vascular smooth muscle cells going from proliferative motile circular contractile fusiform show...
Abstract Dendritic spines are small subcompartments that protrude from the dendrites of neurons and important for signaling activity synaptic communication. These have been characterized to different shapes. While it is known these shapes associated with spine function, specific nature shape-function relationships not well understood. In this work, we systematically investigated relationship between shape size both head apparatus, a specialized endoplasmic reticulum compartment in head,...
Abstract The modification of neural circuits depends on the strengthening and weakening synaptic connections. Synaptic strength is often correlated to density ionotropic, glutamatergic receptors, AMPARs, (α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptors) at postsynaptic (PSD). While AMPAR known change based complex biological signalling cascades, effect geometric factors such as dendritic spine shape, size curvature remain poorly understood. In this work, we developed a...
Abstract Dendritic spines are small, bulbous protrusions along the dendrites of neurons and sites excitatory postsynaptic activity. The morphology has been implicated in their function synaptic plasticity shapes have well-characterized, but potential mechanics underlying shape development maintenance not yet fully understood. In this work, we explore mechanical principles that could underlie specific using a minimal biophysical model membrane-actin interactions. Using model, first identify...
Abstract The modification of neural circuits depends on the strengthening and weakening synaptic connections. Synaptic strength is often correlated to density ionotropic, glutamateric receptors, AMPAR, ( α -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) at postsynaptic (PSD). While AMPAR known change based complex biological signaling cascades, effect geometric factors such as dendritic spine shape, size, curvature remain poorly understood. In this work, we developed a...
Abstract Synaptic plasticity involves the modification of both biochemical and structural components neurons. Many studies have revealed that change in number density glutamatergic receptor AMPAR at synapse is proportional to synaptic weight update; increase corresponds strengthening synapses while decrease weakens connections. The dynamics are thought be regulated by upstream signaling, primarily calcium-CaMKII pathway, trafficking from synapse, influx extrasynaptic sources. Here, we...
Abstract Dendritic spines act as biochemical computational units and must adapt their responses according to activation history. Calcium influx acts the first signaling step during post-synaptic is a determinant of synaptic weight change. also come in variety sizes shapes. To probe relationship between calcium dynamics spine morphology, we used stochastic reaction-diffusion model idealized realistic geometries. We show that despite nature various channels, receptors, pumps, size shape can...