Mesenchymal stem cells (MSC) can differentiate into several cell types and are desirable candidates for therapy tissue engineering. However, due to poor survival, proliferation differentiation in the patient, outcomes have not been satisfactory. Although studies done understand conditions that promote proliferation, migration of MSC vitro vivo, still there is no clear understanding on effect non-cellular bio molecules. Of many factors influence behavior, immediate microenvironment plays a...

Abstract Background Mesenchymal Stem Cells (MSC) are important candidates for therapeutic applications due to their ex vivo proliferation and differentiation capacity. MSC is controlled by both intrinsic extrinsic factors actin cytoskeleton plays a major role in the event. In current study, we tried understand initial molecular mechanisms pathways that regulate of into osteocytes or adipocytes. Results We observed modification was during differentially regulated adipogenesis osteogenesis....

Previous reports have suggested that the protooncogene c-myb participates in T cell development thymus and mature proliferation. We generated two cell-specific knockout mouse models, myb/LckCre myb/CD4Cre. demonstrated is required for of thymocytes at DN3 stage, survival proliferation double-positive thymocytes, differentiation single-positive CD4 CD8 cells, proliferative responses cells. In addition, our data show directly involved formation CD4+CD8+CD25+, CD4+CD25+, CD8+CD25+ developmental...

Mesenchymal stem cells (MSC) are used for several therapeutic applications to improve the functions of bone, cardiac, nervous tissue as well facilitate repopulation hematopoietic cells. MSC give rise non-hematopoietic stromal bone marrow and important maintenance normal hematopoiesis. Chemotherapeutic drugs treatment leukemia extensively damage alter their gene expression profiles.We determined changes in adipogenic, osteogenic differentiation, phenotypic during with chemotherapeutic...

<b><i>Introduction:</i></b> Metastatic breast cancer has poor prognosis due to limited therapeutic options. Protein kinase dysregulations have a major role in progression and metastasis. In this study, we investigated the anti-cancer activity of sorafenib, multikinase inhibitor, which targets receptor tyrosine kinases cancer. Although treatment with sorafenib increased patient survival inhibited metastatic migration hepatocellular carcinoma, its migration, metastasis,...

Chronic myeloid leukemia (CML) is characterized by abnormal proliferation of cells which when untreated leads to bone marrow failure. Imatinib mesylate (IM) the first line therapy for treatment CML and results in remission most cases. However, a significant percentage patients develop chemoresistance IM, might be due presence chemoresistant marrow. In current study, we explored role cell-cell interaction with stromal development CML. We found that offered long-term chemoprotection from...

Enteral feeding with small amounts to stimulate bowel motility, and glutamine supplementation, which provides nutrients selectively used by intestinal epithelial cells, might preserve the gut mucosa during fasting. We evaluated effects of interaction between mechanical strain supplementation in human Caco-2 pursued finding equivalent l- d-glutamine Caco-2, HT-29, primary malignant colonocytes. cells were subjected repetitive media containing 2 mmol/L l-glutamine supplemented or d-glutamine....

The c-myb proto-oncogene encodes two alternatively spliced mRNAs, which in turn code for proteins of 75 kDa and 89 kDa. It is at present unclear whether the isoforms c-Myb perform identical functions or they mediate different biological effects. To assess their role apoptotic death hematopoietic cells, we expressed murine myeloid cell lines 32Dcl3 FDCP1. Our results show that while ectopic overexpression p75 acceleration death, similar p89 protection cells from death. An analysis gene...

An IL-4-dependent T cell clone (LD8) was isolated from the murine IL-2-dependent cytotoxic line C30.1. This has lost capacity to proliferate in response IL-2 after long-term culture IL-4. LD8 cells express p70, but not p55, subunit of IL-2R on their surface. The number p70 molecules is comparable with high-affinity parental C30.1 line. can efficiently internalize through subunit. Following stimulation by IL-2, up-regulate mRNA, do p55 mRNA. proliferation reconstituted introduction and...

Hematopoietic stem/progenitor cells (HSPCs) maintain the hematopoietic system by balancing their self-renewal and differentiation events. stem also migrate to various sites interact with specific microenvironment integrity of system. Rho GTPases have been found control migration other cell types. Although role RAC1, RAC2 CDC42 has studied, RHOA in human is unclear. By utilizing constitutively active dominant negative RHOA, we show that negatively regulates both vitro vivo significantly...

Mesenchymal stem cells (MSC) have been proposed as suitable candidates for cell therapy neurological disorderssince they exhibit good neuronal differentiation capacity. However, better therapeutic outcomes, it is necessary to isolate MSC from a tissue sourcethat posses high differentiation. In this context, we isolated extra ocular muscle (EOM) and tested the in vitro potential. current study, EOM derived were characterized compared with bone marrow MSC. We found that proliferated monolayer...

The c-myb proto-oncogene plays a central role in hematopoiesis and encodes major translational product of 75 kDa. c-Myb is highly expressed immature hematopoietic cells, its expression down-regulated during terminal differentiation. Deregulated has been shown to block differentiation cells. Here we have studied the mechanism action nature target genes through which mediates 32Dcl3 murine myeloid We show that ectopic overexpression cells results c-Myc. However, enforced c-Myc did not alter...

Protein phosphorylation is one of the most important post-translational modifications, which acts as a reversible on or off switch for activity large number proteins. Analyzing status different proteins can reveal alterations in state cells response to cellular damage, cancer and pharmaceutical drugs. Techniques such mass spectrometry, radiolabeling, 2D-gel electrophoresis western blotting are used quantify protein phosphorylation. These assays bulk population cells, however, flow cytometry...

Abstract Aurora-A kinase is a serine/threonine that essential for faithful chromosome segregation during mitosis. A single nucleotide polymorphism T91A (amino acid Phe31→Ile) present in the coding region of human associated with susceptibility to multiple cancer types, yet precise biochemical mechanisms by which these different polymorphisms function are still not well characterized. To investigate functional differences between two alleles Aurora-A, we have generated knock-in mouse carries...