Extracellular signals are transduced into cells through mitogen-activated protein kinases (MAPKs), which activated by their upstream kinases. Recently, families of scaffolding proteins have been identified to tether specific combinations these along signaling pathways. Here we describe a protein, JLP (c-Jun NH2-terminal kinase-associated leucine zipper protein), acts as bring together Max and c-Myc with JNK kinase) p38MAPK, well MKK4 (MAPK kinase 4) MEKK3 3). Thus, defines family that MAPKs...

The p38 mitogen-activated protein kinase (MAPK) pathway plays an important role in cell differentiation, but the signaling mechanisms by which it is activated during this process are largely unknown. Cdo immunoglobulin superfamily member that functions as a component of multiprotein surface complexes to promote myogenesis. In study, we report intracellular region interacts with JLP, scaffold for p38alpha/beta MAPK pathway. Cdo, and form differentiating myoblasts, JLP cooperate enhance levels...

Electroencephalography (EEG) is widely used by clinicians, scientists, engineers and other professionals worldwide, with an increasing number of low-cost, commercially-oriented EEG systems that have become available in recent years. One such system the Cognionics Quick-20 (Cognionics Inc., San Diego, USA), which uses dry electrodes offers convenience portability thanks to its built-in amplifier wireless connection. Because characteristics, this has been several applications for both clinical...

Tubulin, the major structural component of microtubules, is a target for development anticancer agents. A series (Z)-1-aryl-3-arylamino-2-propen-1-one (10) were synthesized and evaluated antiproliferative activity in cell-based assay. The most active compound (Z)-1-(2-bromo-3,4,5-trimethoxyphenyl)-3-(3-hydroxy-4-methoxyphenylamino)prop-2-en-1-one (10ae) was tested 20 tumor cell lines including multidrug resistant phenotype found to induce apoptosis all these with similar GI(50) values. Flow...

Scaffolding proteins exist in eukaryotes to properly assemble signaling into specific multimeric functional complexes. JLP is a novel leucine zipper protein belonging family of scaffolding that JNK modules. proline-rich contains two domains and highly conserved C-terminal domain. We have identified kinesin light chain 1 (KLC1) as binding partner for the second domain using yeast two-hybrid screening. The interaction KLC1 was mapped its tetratripeptide repeat, which zipper-like crucial with...

Many cruciferous vegetables, including cabbage, contain indole-3-carbinol (I3C), which is a known anticarcinogen. However, the anticarcinogenic effects of I3C on liver cancer have not been investigated. Therefore, this study was conducted to evaluate in human hepatocellular carcinoma (HCC) SNU449 cells. The results MTT and WST-1 assays indicated that treatment cells with decreased viability dose- time-dependent manners, while colony formation also inhibited proliferation Moreover,...

Chemokine receptors control several fundamental cellular processes in both hematopoietic and structural cells, including directed cell movement, i.e., chemotaxis, differentiation, proliferation. We have previously demonstrated that CXCR3, the chemokine receptor expressed by Th1/Tc1 inflammatory cells present lung, is also human airway epithelial cells. In activation of CXCR3 induces movement proliferation, underlie lung repair. The study examined expression function alveolar type II...

Scaffolding proteins play a critical role in conferring specificity and fidelity to signaling pathways. The JNK-interacting leucine zipper protein (JLP) has been identified as scaffolding involved linking components of the JNK module. Gα12 Gα13, α-subunits heterotrimeric G G12 G13, respectively, stimulate module diverse cell types. Here, we report that Gα13 physically interacts with JLP, this interaction enhances Gα13-mediated activation. We also demonstrate endogenous between JLP MCF-7...

JLP (JNK-associated leucine zipper protein) is a novel scaffolding protein involved in JNK signaling. Although it known that highly expressed brain, the biological function of neuronal systems remains unknown. Here, we report interaction between and SCG10 (superior cervical ganglia clone 10), which microtubule-destabilizing factor essential for neurite outgrowth. Inhibition endogenous expression using small interference RNA methodology strongly enhanced nerve growth (NGF)-induced outgrowth...

Abstract Persistent luminescence (PersL) is an optical phenomenon which allows for materials to emit after ceasing excitation. The long‐lasting ascribed the presence of trap states, can be exploited through introduction various dopants and post‐synthesis treatment. In this study, ZnGa 2 O 4 : Cr 3+ (CZGO), one most widely investigated near‐infrared‐emitting PersL materials, synthesized in form nanoparticles, incorporated into amorphous calcium phosphate (ACP) a luminescent nanocomposite with...

Large-scale location based traces, such as mobile phone data, have been identified a promising data source to complement or even enrich official statistics. In many cases, prerequisite step deploy the massively gathered is detection of home from individual users. The problem that little research exists on validation (comparison with ground truth datasets) uncertainty estimation methods, not at user level, nor nation-wide levels. this paper, we present an extensive empirical analysis methods...

The c-myb proto-oncogene encodes two alternatively spliced mRNAs, which in turn code for proteins of 75 kDa and 89 kDa. It is at present unclear whether the isoforms c-Myb perform identical functions or they mediate different biological effects. To assess their role apoptotic death hematopoietic cells, we expressed murine myeloid cell lines 32Dcl3 FDCP1. Our results show that while ectopic overexpression p75 acceleration death, similar p89 protection cells from death. An analysis gene...

Fluorescent quantum dots (semiconductor nanocrystals) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining that are water soluble and biocompatible. The objectives of our research were develop a methodology for encapsulation cadnium-selenium (CdSe) (QDs) phospholipid nanoemulsion mimics natural lipoprotein core study interactions with cultured non-small cell lung cancer cells (NSCLC). We found CdSe QDs can be efficiently...

The activated mutants of the α-subunits G proteins G12 and G13 have been designated as gep oncogenes owing to their ability stimulate diverse oncogenic signaling pathways that lead neoplastic transformation fibroblast cell lines tumorigenesis in nude mice models. Studies from our laboratory well others shown growth-promoting activities Gα12 Gα13 involve potent activation c-Jun N-terminal kinases (JNKs). Our previous studies indicated JNK-interacting leucine zipper protein (JLP), a scaffold...

Indole-3-carbinol (I3C) is a phytochemical that exhibits growth-inhibitory activity against various cancer cells. However, there are limited studies on the effects of I3C colon In this study, human colorectal carcinoma cell line (LoVo) was examined. The results 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide, colony formation, and counting assays revealed suppressed proliferation LoVo Microscopy wound-healing analyses affected morphology inhibited migration cells, respectively....

JLP (JNK-associated leucine zipper protein) is a scaffolding protein that interacts with various signaling proteins associated coordinated regulation of cellular process such as endocytosis, motility, neurite outgrowth, cell proliferation, and apoptosis. Here we identified PLK1 (Polo-like kinase 1) novel interaction partner through mass spectrometric approaches. Our results indicate phospho-primed by on Thr-351, which recognized the Polo box domain leading to phosphorylation at additional...