A5086740780- Mosquito-borne diseases and control
- Viral Infections and Vectors
- CAR-T cell therapy research
- Virology and Viral Diseases
- Virus-based gene therapy research
- HIV Research and Treatment
- Hemophilia Treatment and Research
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Chronic Myeloid Leukemia Treatments
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
- Viral Infectious Diseases and Gene Expression in Insects
- Antibiotic Use and Resistance
- T-cell and B-cell Immunology
- Genetic and phenotypic traits in livestock
- Viral Infections and Outbreaks Research
- Plant Pathogens and Fungal Diseases
- Diet, Metabolism, and Disease
- Endometrial and Cervical Cancer Treatments
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Pancreatic function and diabetes
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Indiana University School of Medicine
2019-2025
Indiana University – Purdue University Indianapolis
2019-2025
The Charutar Vidya Mandal (CVM) University
2024
Shree Guru Gobind Singh Tricentenary University
2021-2023
Indianapolis Zoo
2023
International Centre for Genetic Engineering and Biotechnology
2017-2019
Jaypee Institute of Information Technology
2011-2018
National Bureau of Plant Genetic Resources
2018
Gandhi Medical College & Hospital
2016
First Affiliated Hospital of Zhengzhou University
2014
Regulatory T cells (Tregs) control immune responses in autoimmune disease, transplantation, and enable antigen-specific tolerance induction protein-replacement therapies. Tregs can exert a broad array of suppressive functions through their cell receptor (TCR) tissue-directed manner. This capacity now be harnessed for by "redirecting" polyclonal to overcome low inherent precursor frequencies simultaneously augment functions. With the use hemophilia A as model, we sought engineer suppress...
Hepatic adeno-associated viral (AAV) gene transfer has the potential to cure X-linked bleeding disorder hemophilia A. However, declining therapeutic coagulation factor VIII (FVIII) expression plagued clinical trials. To assess mechanistic underpinnings of this loss FVIII expression, we developed a A mouse model that shares key features observed in Following liver-directed AAV8 presence rapamycin, initial protein declines over time absence antibody formation. Surprisingly, production occurs...
Adeno-associated virus (AAV) vectors are used for correcting multiple genetic disorders. Although the goal is to achieve lifelong correction with a single vector administration, ability redose would enable extension of therapy in cases which initial gene transfer insufficient lasting cure, episomal forms lost growing organs pediatric patients, or transgene expression diminished over time. However, AAV typically induces potent and long-lasting neutralizing antibodies (NAbs) against capsid...
The development of inhibitory antibodies (inhibitors) is a serious complication in the treatment hemophilia A with clotting factor VIII (FVIII) replacement therapy. Inhibitor formation critically depends on T cell help and modulation by regulatory cells (Tregs). In this study, we evaluated F5111 immunocytokine (IC), single chain fusion between human interleukin-2 (IL-2) cytokine an IL-2 antibody that biases activity towards high receptor alpha (IL-2Rα) expression, leading to extended...
Dengue virus (DENV), the causative agent of dengue disease, is among most important mosquito-borne pathogens worldwide. DENV composed four closely related serotypes and belongs to Flaviviridae family alongside other arthropod-borne viral such as Zika (ZIKV), West Nile (WNV) Yellow Fever (YFV). After infection, antibody response mostly directed E glycoprotein which three structural domains named DI, DII DIII that share variable degrees homology different viruses. Recent evidence supports a...
The flavivirus capsid protein (C) is separated from the downstream premembrane (PrM) by a hydrophobic sequence named anchor (Ca). During polyprotein processing, Ca sequentially cleaved viral NS2B/NS3 protease on cytosolic side and signal peptidase luminal of endoplasmic reticulum (ER). To date, considered important mostly for directing translocation PrM into ER lumen. In this study, role in assembly secretion Zika virus was investigated using pseudovirus-based approach. Our results show...
Interpretation :The inter-relationships between the traits suggested that accessions with short flowering and maturity duration, low plant height, large bolls bold seeds should be given priority in breeding for enhanced yield.Donors various were identified which may used future linseed to target yield enhancement diverse geographical adaptation.
Abstract Dengue and Zika are two of the most important human viral pathogens worldwide. In both cases, envelope glycoprotein E is main target antibody response. Recently, new complex quaternary epitopes were identified which consequence arrangement antiparallel dimers on surface. Such can be exploited to develop more efficient cross-neutralizing vaccines. Here we describe a successful covalent stabilization from viruses in mammalian cells. Folding dimerization secretory was found strongly...
Limitations to successful gene therapy with adeno-associated virus (AAV) can comprise pre-existing neutralizing antibodies the vector capsid that block cellular entry, or inefficient transduction of target cells lead sub-optimal expression therapeutic transgene. Recombinant serotype 3 AAV (AAV3) is an emerging candidate for liver-directed therapy. In this study, we integrated rational design by using a combinatorial library derived from AAV3B capsids directed evolution in vitro selection...
Formation of virus specific replicase complex is among the most important steps that determines fate viral transcription and replication during Chikungunya (CHIKV) infection. In present study, authors have computationally generated a 3D structure CHIKV late on basis interactions identified domains nonstructural proteins (nsPs) which make up complex. The nsPs were using systems such as pull down, protein interaction ELISA, yeast two-hybrid. structures I-TASSER biological assembly was...
Hemophilia A is an inherited coagulation disorder resulting in the loss of functional clotting factor VIII (FVIII). Presently, most effective treatment prophylactic protein replacement therapy. However, this requires frequent life-long intravenous infusions plasma derived or recombinant factors and not a cure. major complication development inhibitory antibodies that nullify factor. Immune tolerance induction (ITI) therapy to reverse inhibitors can last from months years, daily every other...
Chandipura virus (CHPV), alike other pathogens, exploits the cellular infrastructure of their hosts through complex network interactions for successful infection. CHPV being a recently emerged pediatric encephalitic virus, mechanisms involved in establishment viral persistence are still ill defined. Because protein interface between and its host provides one means by which invades seize control human machinery, authors this study have employed computational methods to create putative...
Tyrosine protein-kinase 2 (TYK2), a member of the Janus kinase family, mediates inflammatory signaling through multiple cytokines, including interferon-α (IFNα), interleukin (IL)-12, and IL-23. Missense mutations in TYK2 are associated with protection against type 1 diabetes (T1D), inhibition shows promise management other autoimmune conditions. Here, we evaluated effects specific inhibitors (TYK2is) pre-clinical models T1D. First, human β cells, cadaveric donor islets, iPSC-derived islets...
Capsid engineering of adeno-associated virus (AAV) can surmount current limitations to gene therapy such as broad tissue tropism, low transduction efficiency, or pre-existing neutralizing antibodies (NAb) that restrict patient eligibility. We previously generated an AAV3B combinatorial capsid library by integrating rational design and directed evolution with the aim improving hepatotropism. A potential isolate, AAV3B-DE5, gained a selective proliferative advantage over five rounds iterative...
The nucleocapsid (N) protein of Chandipura virus (CHPV) plays a crucial role in viral life cycle, besides being an important structural component the virion through proper organization its interactions with other proteins. In recent study, authors had mapped associations among CHPV proteins and shown that N interacts four proteins: N, phosphoprotein (P), matrix (M), glycoprotein (G). present study aimed to distinguish regions responsible for this direction, we have generated structure by...