A5037745948- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- CRISPR and Genetic Engineering
- Folate and B Vitamins Research
- Selenium in Biological Systems
- Trace Elements in Health
- Infectious Diseases and Tuberculosis
- Virus-based gene therapy research
- RNA regulation and disease
- Pneumocystis jirovecii pneumonia detection and treatment
- Radiology practices and education
- Muscle Physiology and Disorders
- Inflammasome and immune disorders
- Biochemical and Molecular Research
- Otolaryngology and Infectious Diseases
- Iron Metabolism and Disorders
- HIV-related health complications and treatments
- Synthesis and Biological Evaluation
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- PI3K/AKT/mTOR signaling in cancer
- RNA Interference and Gene Delivery
- Healthcare Policy and Management
- RNA Research and Splicing
- Antibiotic Resistance in Bacteria
University of Massachusetts Chan Medical School
2018-2023
Louisiana State University Health Sciences Center New Orleans
2017-2023
University at Buffalo, State University of New York
2022
University of Central Florida
2020-2021
UCLA Health
2016
Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need prepare double-stranded DNA (dsDNA) substrates. A synthetic "targeting oligonucleotide" is incorporated into chromosome via homologous mediated by phage Che9c RecT annealase. This oligonucleotide contains site-specific site directional Bxb1 integrase (Int), which allows simultaneous integration of "payload plasmid" cognate and selectable marker. The targeting...
Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic genome editing but severely constrained by cargo limits. Simultaneous of multiple can limit dose and efficacy increase safety risks. Here, we describe single-vector, ~4.8-kb AAV that express Nme2Cas9 either two sgRNAs segmental deletions, or a single sgRNA with homology-directed repair (HDR) template. We also use anti-CRISPR proteins to enable production self-inactivate via cleavage. further introduce...
In 2017 over 550,000 estimated new cases of multi-drug/rifampicin resistant tuberculosis (MDR/RR-TB) occurred, emphasizing a need for treatment strategies. Linezolid (LZD) is potent antibiotic drug-resistant Gram-positive infections and an effective TB. However, extended LZD use can lead to LZD-associated host toxicities, most commonly bone marrow suppression. toxicities may be mediated by IL-1, inflammatory pathway important early immunity during M. infection. IL-1 contribute pathology...
is exposed to a variety of stresses during chronic infection, as the immune system simultaneously produces bactericidal compounds and starves pathogen essential nutrients. The intramembrane protease, Rip1, plays an important role in adaptation these stresses, at least partially by cleavage membrane-bound transcriptional regulators. Although Rip1 known be critical for surviving copper intoxication nitric oxide exposure, do not fully account regulatory protein's essentiality infection. In this...
ABSTRACT Current methods for genome engineering in mycobacteria rely on relatively inefficient recombination systems that require the laborious construction of a long double-stranded DNA substrate each desired modification. We combined two efficient to produce versatile method high-throughput chromosomal obviates need preparation substrates. A synthetic “targeting oligonucleotide” is incorporated into chromosome via homologous mediated by phage Che9c RecT annelase. This oligo contains...
Abstract In 2017 over 550,000 estimated new cases of multi-drug/rifampicin resistant tuberculosis (MDR/RR-TB) occurred, emphasizing a need for treatment strategies. Linezolid (LZD) is potent antibiotic drug-resistant Gram-positive infections and an effective TB. However, extended LZD use can lead to LZD-associated host toxicities, most commonly bone marrow suppression. toxicities may be mediated by IL-1, inflammatory pathway important early immunity during M. infection. IL-1 contribute...
Abstract Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic genome editing but severely constrained by cargo limits, especially large effectors like Cas9s. Simultaneous of multiple can limit dose and efficacy increase safety risks. The use compact has enabled single-AAV Cas9s with 1-3 guides edits that end-joining repair pathways, many precise correct disease-causing mutations in vivo require homology-directed (HDR) templates. Here, we describe...
ABSTRACT Selenium is a vital micronutrient in many organisms. While traces are required for utilization by the microbe, excess amounts toxic; thus, selenium can be regarded as biological “double-edged sword”. chemically similar to essential element sulfur, but curiously, evolution has selected former over latter subset of oxidoreductases. Enzymes involved sulfur metabolism less discriminate terms preventing incorporation; however, its specific incorporation into selenoproteins reveals highly...
ABSTRACT The eukaryotic translation initiation factor 4A (eIF4A) resolves mRNA structures to support protein synthesis, yet little is known about its regulation. Here we analyzed eIF4A phosphorylation during alternate stages of the cell cycle, and found three residues near DEAD box motif (T73, T146, S177) underwent substantial changes. Phosphomimetic mutations T73D T146D led G2/M phase arrest, abolished interaction with RNA, suggesting activity needed for completion division. In addition...