A5013036859- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Liver Diseases and Immunity
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Hepatocellular Carcinoma Treatment and Prognosis
- Adipokines, Inflammation, and Metabolic Diseases
- Sirtuins and Resveratrol in Medicine
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Autoimmune and Inflammatory Disorders
- Electrolyte and hormonal disorders
- Streptococcal Infections and Treatments
- Venous Thromboembolism Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Electroconvulsive Therapy Studies
- Infections and bacterial resistance
- Hemophilia Treatment and Research
- Bacterial Identification and Susceptibility Testing
- Lipid metabolism and biosynthesis
- Central Venous Catheters and Hemodialysis
- Clinical Reasoning and Diagnostic Skills
- Potassium and Related Disorders
University of Massachusetts Chan Medical School
2018-2024
UMass Memorial Medical Center
2024
The University of Sydney
2023
University Hospital of Heraklion
2017-2021
University of Crete
2018-2020
Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic genome editing but severely constrained by cargo limits. Simultaneous of multiple can limit dose and efficacy increase safety risks. Here, we describe single-vector, ~4.8-kb AAV that express Nme2Cas9 either two sgRNAs segmental deletions, or a single sgRNA with homology-directed repair (HDR) template. We also use anti-CRISPR proteins to enable production self-inactivate via cleavage. further introduce...
Abstract Obesity and type 2 diabetes are associated with disturbances in insulin-regulated glucose lipid fluxes severe comorbidities including cardiovascular disease steatohepatitis. Whole body metabolism is regulated by lipid-storing white adipocytes as well “brown” “brite/beige” that express thermogenic uncoupling protein 1 (UCP1) secrete factors favorable to metabolic health. Implantation of brown fat into obese mice improves tolerance, but translation humans has been stymied low...
Disruption of adipocyte de novo lipogenesis (DNL) by deletion fatty acid synthase (FASN) in mice induces browning inguinal white adipose tissue (iWAT). However, FASN knockout (KO) increases acetyl-coenzyme A (CoA) and malonyl-CoA addition to depletion palmitate. We explore which these metabolite changes triggers generating eight adipose-selective KO mouse models with loss ATP-citrate lyase (ACLY), acetyl-CoA carboxylase 1 (ACC1), ACC2, decarboxylase (MCD) or FASN, dual KOs ACLY/FASN,...
Hyponatremia is the most common electrolyte disorder, commonly affecting older hospitalized individuals; however, literature not clear regarding its effect on mortality. The aim of this 2-year observational prospective cohort study was to evaluate mortality and re-admission rates, clinical laboratory characteristics causes hyponatremia in patients than 65 years admitted with a corrected serum sodium 130 mEq/L or less an internal medicine ward tertiary Greek university hospital. During...
Circulating lactate is a fuel source for liver metabolism but may exacerbate metabolic diseases such as nonalcoholic steatohepatitis (NASH). Indeed, haploinsufficiency of transporter monocarboxylate 1 (MCT1) in mice reportedly promotes resistance to hepatic steatosis and inflammation. Here, we used adeno-associated virus (AAV) vectors deliver thyroxin binding globulin (TBG)-Cre or lecithin-retinol acyltransferase (Lrat)-Cre MCT1 fl/fl on choline-deficient, high-fat NASH diet deplete...
Circulating lactate is a fuel source for liver metabolism but may exacerbate metabolic diseases such as nonalcoholic steatohepatitis (NASH). Indeed, haploinsufficiency of transporter monocarboxylate 1 (MCT1) in mice reportedly promotes resistance to hepatic steatosis and inflammation. Here, we used adeno-associated virus (AAV) vectors deliver thyroxin binding globulin (TBG)-Cre or lecithin-retinol acyltransferase (Lrat)-Cre MCT1 fl/fl on choline-deficient, high-fat NASH diet deplete...
Abstract Circulating lactate is a fuel source for liver metabolism but may exacerbate metabolic diseases such as nonalcoholic steatohepatitis (NASH). Indeed, haploinsufficiency of transporter monocarboxylate 1 (MCT1) in mice reportedly promotes resistance to hepatic steatosis and inflammation. Here, we used adeno-associated virus (AAV) vectors deliver thyroxin binding globulin (TBG)-Cre or lecithin-retinol acyltransferase (Lrat)-Cre MCT1 fl/fl on choline deficient, high fat NASH diet deplete...
Nuclear receptor interacting protein 1 (NRIP1) suppresses energy expenditure via repression of nuclear receptors, and its depletion markedly elevates uncoupled respiration in mouse human adipocytes. We tested whether NRIP1 deficient adipocytes implanted into obese mice would enhance whole body metabolism. Since β-adrenergic signaling through cAMP strongly promotes adipocyte thermogenesis, we the effects knock-out (NRIP1KO) require pathway.
Abstract Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic genome editing but severely constrained by cargo limits, especially large effectors like Cas9s. Simultaneous of multiple can limit dose and efficacy increase safety risks. The use compact has enabled single-AAV Cas9s with 1-3 guides edits that end-joining repair pathways, many precise correct disease-causing mutations in vivo require homology-directed (HDR) templates. Here, we describe...
Abstract Obesity and type 2 diabetes (T2D) are associated with poor tissue responses to insulin 1,2 , disturbances in glucose lipid fluxes 3–5 comorbidities including steatohepatitis 6 cardiovascular disease 7,8 . Despite extensive efforts at prevention treatment 9,10 afflicts over 400 million people worldwide 11 Whole body metabolism is regulated by adipose depots 12–14 which include both lipid-storing white adipocytes less abundant “brown” “brite/beige” that express thermogenic uncoupling...
Circulating lactate is a fuel source for liver metabolism but may exacerbate metabolic diseases such as nonalcoholic steatohepatitis (NASH). Indeed, haploinsufficiency of transporter monocarboxylate 1 (MCT1) in mice reportedly promotes resistance to hepatic steatosis and inflammation. Here, we used adeno-associated virus (AAV) vectors deliver thyroxin binding globulin (TBG)-Cre or lecithin-retinol acyltransferase (Lrat)-Cre MCT1 fl/fl on choline deficient, high fat NASH diet deplete...
Circulating lactate is a fuel source for liver metabolism but may exacerbate metabolic diseases such as nonalcoholic steatohepatitis (NASH). Indeed, haploinsufficiency of transporter monocarboxylate 1 (MCT1) in mice reportedly promotes resistance to hepatic steatosis and inflammation. Here, we used adeno-associated virus (AAV) vectors deliver thyroxin binding globulin (TBG)-Cre or lecithin-retinol acyltransferase (Lrat)-Cre MCT1 fl/fl on choline deficient, high fat NASH diet deplete...
imaging results total resolution of UTI was achieved in patients who had for several months and even years.CONCLUSIONS: Combined PET/MR with (68) Ga-pentixafor is highly accurate to localize leukocyte accumulation after kidney transplantation recurrent complicated UTI.Identification the localization might alter therapeutic decision patients.
Acquired hemophilia is a rare but potentially fatal clinical condition requiring suspicion to reach diagnosis, especially in elder patients. This diagnosis should be suspected patients that present with unexplained persistent bleeding from skin, soft tissues, and mucosa have prolonged aPTT.
ABSTRACT RNA-guided engineered nucleases derived from a prokaryotic adaptive immune system known as CRISPR-Cas represent promising platform for gene deletion and editing. As therapeutic approach, direct delivery of Cas9 protein guide RNA could circumvent the safety problems associated with plasmid therefore represents an attractive tool genome engineering. Gene or editing in adipose tissue to enhance its energy expenditure, fat oxidation secretion bioactive factors through “browning” process...